Construct: ORF TRCN0000466169
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003259.1_s317c1
- Derived from:
- ccsbBroadEn_01953
- DNA Barcode:
- ATCAAATCTGACTGTCACGGCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIAS1 (8554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466169
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8554 | PIAS1 | protein inhibitor of activa... | NM_016166.2 | 100% | 100% | |
| 2 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_011522127.2 | 99% | 98.7% | 4_5insCGGACAGTGCGGAACTAA |
| 3 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_017022689.1 | 98.8% | 98.7% | 0_1ins21;2T>A |
| 4 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_017022690.1 | 98.6% | 98.6% | 0_1ins27 |
| 5 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_017022691.2 | 98.6% | 98.6% | 0_1ins27 |
| 6 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_011522126.2 | 98.1% | 96.6% | (many diffs) |
| 7 | human | 8554 | PIAS1 | protein inhibitor of activa... | NM_001320687.1 | 98% | 98.7% | 1_22del;25_26insGCGGACAGTGCGGAAC;27T>A |
| 8 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_017022688.1 | 98% | 98.7% | 1_22del;25_26insGCGGACAGTGCGGAAC;27T>A |
| 9 | human | 8554 | PIAS1 | protein inhibitor of activa... | XM_024450094.1 | 71.7% | 71.7% | 0_1ins552 |
| 10 | mouse | 56469 | Pias1 | protein inhibitor of activa... | NM_019663.3 | 90.6% | 98% | (many diffs) |
| 11 | mouse | 56469 | Pias1 | protein inhibitor of activa... | XM_006511302.3 | 89.2% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2022
- ORF length:
- 1953
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggacagt gcggaactaa agcaaatggt tatgagcctt agagtttctg 121 aactccaagt actgttgggc tacgccggga gaaacaagca cggacgcaaa cacgaacttc 181 tcacaaaagc cctgcatttg ctaaaggctg gctgtagtcc tgctgtgcaa atgaaaatta 241 aggaactcta taggcggcgg ttcccacaga aaatcatgac gcctgcagac ttgtccatcc 301 ccaacgtaca ttcaagtcct atgccagcaa ctttgtctcc atctaccatt ccacaactca 361 cttacgatgg tcaccctgca tcatcgccat tactccctgt ttctcttctg ggacctaaac 421 atgaactgga actcccacat cttacatcag ctcttcaccc agtccatccg gatataaaac 481 ttcaaaaatt accattttat gatttactgg atgaactgat aaaacccacc agtctagcat 541 cagacaacag tcagcgcttt cgagaaacct gttttgcatt tgccttgaca ccacaacaag 601 tgcagcaaat cagtagttcc atggatattt ctgggaccaa atgtgacttc acagtacagg 661 tccagttaag gttttgttta tcagaaacca gttgtccaca agaagatcac ttcccaccca 721 atctttgtgt gaaagtgaat acaaaacctt gcagccttcc aggttacctt ccacctacaa 781 aaaatggcgt ggaaccaaag cgacccagcc gaccaattaa tatcacctca cttgtccgac 841 tgtccacaac agtaccaaac acgattgttg tttcttggac tgcagaaatt ggaagaaact 901 attccatggc agtatatctt gtaaaacagt tgtcctcaac agttcttctt cagaggttac 961 gagcaaaggg aataaggaat ccggatcatt ctagagcttt aattaaagag aagttgactg 1021 cggatccaga cagtgaaata gctacaacca gcctaagggt ttctctacta tgtccacttg 1081 gtaaaatgcg gctgacaatt ccgtgtcggg cccttacatg ttctcatcta caatgttttg 1141 acgcaactct ttacattcag atgaatgaga aaaaaccaac ctgggtttgt cctgtctgtg 1201 ataagaaggc tccatatgaa caccttatta ttgatggctt gtttatggaa atcctaaagt 1261 actgtacaga ctgtgatgaa atacaattta aggaggatgg cacttgggca ccgatgagat 1321 caaaaaagga agtacaggaa gtttctgcct cttacaatgg agtcgatgga tgcttgagct 1381 ccacattgga gcatcaggta gcgtctcacc accagtcctc aaataaaaac aagaaagtag 1441 aagtgattga cctaaccata gacagttcat ctgatgaaga ggaagaagag ccatctgcca 1501 agaggacctg tccttcccta tctcccacat caccactaaa taataaaggc attttaagtc 1561 ttccacatca agcatctcca gtatcccgca ccccaagcct tcctgctgta gacacaagct 1621 acattaatac ctccctcatc caagactata ggcaTCCTTT CCACATGACA CCCATGCCTT 1681 ACGACTTACA AGGATTAGAT TTCTTTCCTT TCTTATCAGG AGACAATCAG CATTACAACA 1741 CCTCCTTGCT TGCCGCTGCA GCAGCAGCAG TTTCAGATGA TCAAGACCTC CTACACTCGT 1801 CTCGGTTTTT CCCGTATACC TCCTCACAGA TGTTTCTTGA TCAGTTAAGT GCAGGAGGCA 1861 GTACTTCTCT GCCAACCACC AATGGAAGCA GTAGTGGCAG TAACAGCAGC CTGGTTTCTT 1921 CCAACAGCCT AAGGGAAAGC CATAGCCACA CCGTCACAAA CAGGAGCAGC ACGGACACGG 1981 CATCCATCTT TGGCATCATA CCAGACATTA TTTCATTGGA CTTGCCAACT TTCTTGTACA 2041 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2101 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2161 AGGACGAATC AAATCTGACT GTCACGGCAG CACGCGTTAA GTCgacaatc aacctctgga 2221 ttacaaaatt tgtgaaagat t