Construct: ORF TRCN0000466169

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003259.1_s317c1
Derived from:
ccsbBroadEn_01953
DNA Barcode:
ATCAAATCTGACTGTCACGGCAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIAS1 (8554)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466169
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8554 PIAS1 protein inhibitor of activa... NM_016166.2 100% 100%
2 human 8554 PIAS1 protein inhibitor of activa... XM_011522127.2 99% 98.7% 4_5insCGGACAGTGCGGAACTAA
3 human 8554 PIAS1 protein inhibitor of activa... XM_017022689.1 98.8% 98.7% 0_1ins21;2T>A
4 human 8554 PIAS1 protein inhibitor of activa... XM_017022690.1 98.6% 98.6% 0_1ins27
5 human 8554 PIAS1 protein inhibitor of activa... XM_017022691.2 98.6% 98.6% 0_1ins27
6 human 8554 PIAS1 protein inhibitor of activa... XM_011522126.2 98.1% 96.6% (many diffs)
7 human 8554 PIAS1 protein inhibitor of activa... NM_001320687.1 98% 98.7% 1_22del;25_26insGCGGACAGTGCGGAAC;27T>A
8 human 8554 PIAS1 protein inhibitor of activa... XM_017022688.1 98% 98.7% 1_22del;25_26insGCGGACAGTGCGGAAC;27T>A
9 human 8554 PIAS1 protein inhibitor of activa... XM_024450094.1 71.7% 71.7% 0_1ins552
10 mouse 56469 Pias1 protein inhibitor of activa... NM_019663.3 90.6% 98% (many diffs)
11 mouse 56469 Pias1 protein inhibitor of activa... XM_006511302.3 89.2% 96.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2022
ORF length:
1953
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggacagt gcggaactaa agcaaatggt tatgagcctt agagtttctg
121aactccaagt actgttgggc tacgccggga gaaacaagca cggacgcaaa cacgaacttc
181tcacaaaagc cctgcatttg ctaaaggctg gctgtagtcc tgctgtgcaa atgaaaatta
241aggaactcta taggcggcgg ttcccacaga aaatcatgac gcctgcagac ttgtccatcc
301ccaacgtaca ttcaagtcct atgccagcaa ctttgtctcc atctaccatt ccacaactca
361cttacgatgg tcaccctgca tcatcgccat tactccctgt ttctcttctg ggacctaaac
421atgaactgga actcccacat cttacatcag ctcttcaccc agtccatccg gatataaaac
481ttcaaaaatt accattttat gatttactgg atgaactgat aaaacccacc agtctagcat
541cagacaacag tcagcgcttt cgagaaacct gttttgcatt tgccttgaca ccacaacaag
601tgcagcaaat cagtagttcc atggatattt ctgggaccaa atgtgacttc acagtacagg
661tccagttaag gttttgttta tcagaaacca gttgtccaca agaagatcac ttcccaccca
721atctttgtgt gaaagtgaat acaaaacctt gcagccttcc aggttacctt ccacctacaa
781aaaatggcgt ggaaccaaag cgacccagcc gaccaattaa tatcacctca cttgtccgac
841tgtccacaac agtaccaaac acgattgttg tttcttggac tgcagaaatt ggaagaaact
901attccatggc agtatatctt gtaaaacagt tgtcctcaac agttcttctt cagaggttac
961gagcaaaggg aataaggaat ccggatcatt ctagagcttt aattaaagag aagttgactg
1021cggatccaga cagtgaaata gctacaacca gcctaagggt ttctctacta tgtccacttg
1081gtaaaatgcg gctgacaatt ccgtgtcggg cccttacatg ttctcatcta caatgttttg
1141acgcaactct ttacattcag atgaatgaga aaaaaccaac ctgggtttgt cctgtctgtg
1201ataagaaggc tccatatgaa caccttatta ttgatggctt gtttatggaa atcctaaagt
1261actgtacaga ctgtgatgaa atacaattta aggaggatgg cacttgggca ccgatgagat
1321caaaaaagga agtacaggaa gtttctgcct cttacaatgg agtcgatgga tgcttgagct
1381ccacattgga gcatcaggta gcgtctcacc accagtcctc aaataaaaac aagaaagtag
1441aagtgattga cctaaccata gacagttcat ctgatgaaga ggaagaagag ccatctgcca
1501agaggacctg tccttcccta tctcccacat caccactaaa taataaaggc attttaagtc
1561ttccacatca agcatctcca gtatcccgca ccccaagcct tcctgctgta gacacaagct
1621acattaatac ctccctcatc caagactata ggcaTCCTTT CCACATGACA CCCATGCCTT
1681ACGACTTACA AGGATTAGAT TTCTTTCCTT TCTTATCAGG AGACAATCAG CATTACAACA
1741CCTCCTTGCT TGCCGCTGCA GCAGCAGCAG TTTCAGATGA TCAAGACCTC CTACACTCGT
1801CTCGGTTTTT CCCGTATACC TCCTCACAGA TGTTTCTTGA TCAGTTAAGT GCAGGAGGCA
1861GTACTTCTCT GCCAACCACC AATGGAAGCA GTAGTGGCAG TAACAGCAGC CTGGTTTCTT
1921CCAACAGCCT AAGGGAAAGC CATAGCCACA CCGTCACAAA CAGGAGCAGC ACGGACACGG
1981CATCCATCTT TGGCATCATA CCAGACATTA TTTCATTGGA CTTGCCAACT TTCTTGTACA
2041AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2101AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2161AGGACGAATC AAATCTGACT GTCACGGCAG CACGCGTTAA GTCgacaatc aacctctgga
2221ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)