Transcript: Human NM_016166.2

Homo sapiens protein inhibitor of activated STAT 1 (PIAS1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
PIAS1 (8554)
Length:
5743
CDS:
149..2104

Additional Resources:

NCBI RefSeq record:
NM_016166.2
NBCI Gene record:
PIAS1 (8554)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016166.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000257327 ACCCAGTCCATCCGGATATAA pLKO_005 537 CDS 100% 15.000 12.000 N PIAS1 n/a
2 TRCN0000004146 CATCGCCATTACTCCCTGTTT pLKO.1 462 CDS 100% 4.950 3.960 N PIAS1 n/a
3 TRCN0000004144 CCACATCACCACTAAATAATA pLKO.1 1605 CDS 100% 15.000 10.500 N PIAS1 n/a
4 TRCN0000231897 ACTATTCCATGGCAGTATATC pLKO_005 978 CDS 100% 13.200 9.240 N PIAS1 n/a
5 TRCN0000004147 CATGGCAGTATATCTTGTAAA pLKO.1 985 CDS 100% 13.200 9.240 N PIAS1 n/a
6 TRCN0000231899 CGACTTACAAGGATTAGATTT pLKO_005 1762 CDS 100% 13.200 9.240 N PIAS1 n/a
7 TRCN0000231898 CCGGATCATTCTAGAGCTTTA pLKO_005 1061 CDS 100% 10.800 7.560 N PIAS1 n/a
8 TRCN0000010853 CCCATGCCTTACGACTTACAA pLKO.1 1751 CDS 100% 5.625 3.938 N PIAS1 n/a
9 TRCN0000004145 GATCGAATGAACTTGGCAGAA pLKO.1 2138 3UTR 100% 4.050 2.835 N PIAS1 n/a
10 TRCN0000231900 GGCAGAAAGAAGAGAACTTTG pLKO_005 2152 3UTR 100% 10.800 6.480 N PIAS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016166.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01953 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01953 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466169 ATCAAATCTGACTGTCACGGCAGC pLX_317 19.1% 100% 100% V5 n/a
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