Transcript: Mouse XM_006511302.3

PREDICTED: Mus musculus protein inhibitor of activated STAT 1 (Pias1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pias1 (56469)
Length:
6022
CDS:
492..2420

Additional Resources:

NCBI RefSeq record:
XM_006511302.3
NBCI Gene record:
Pias1 (56469)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006511302.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426511 GCGACCTAGCCGACCAATTAA pLKO_005 1196 CDS 100% 15.000 21.000 N Pias1 n/a
2 TRCN0000426103 CCTACGTCACCACTAAGTAAT pLKO_005 1920 CDS 100% 13.200 18.480 N Pias1 n/a
3 TRCN0000430769 CAATTGCATGTATGAGGTTTA pLKO_005 2801 3UTR 100% 10.800 15.120 N Pias1 n/a
4 TRCN0000231898 CCGGATCATTCTAGAGCTTTA pLKO_005 1377 CDS 100% 10.800 15.120 N PIAS1 n/a
5 TRCN0000086240 CCTTATGACTTACAAGGATTA pLKO.1 2073 CDS 100% 10.800 15.120 N Pias1 n/a
6 TRCN0000086238 CCCTCGAATGACAATACACTT pLKO.1 3235 3UTR 100% 4.950 6.930 N Pias1 n/a
7 TRCN0000004147 CATGGCAGTATATCTTGTAAA pLKO.1 1301 CDS 100% 13.200 10.560 N PIAS1 n/a
8 TRCN0000086242 GCGTCCATCTTTGGCATCATA pLKO.1 2376 CDS 100% 5.625 3.938 N Pias1 n/a
9 TRCN0000086241 GCAGAAATTGGAAGAACCTAT pLKO.1 1278 CDS 100% 4.950 3.465 N Pias1 n/a
10 TRCN0000086239 GCTACCATTCTATGACCTGTT pLKO.1 884 CDS 100% 4.050 2.835 N Pias1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006511302.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01953 pDONR223 100% 89.2% 96.6% None (many diffs) n/a
2 ccsbBroad304_01953 pLX_304 0% 89.2% 96.6% V5 (many diffs) n/a
3 TRCN0000466169 ATCAAATCTGACTGTCACGGCAGC pLX_317 19.1% 89.2% 96.6% V5 (many diffs) n/a
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