Construct: ORF TRCN0000466177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011787.1_s317c1
- Derived from:
- ccsbBroadEn_12898
- DNA Barcode:
- ATCTAAACCGTCTGAATTAATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP19 (84984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466177
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84984 | CEP19 | centrosomal protein 19 | NM_032898.5 | 100% | 100% | |
| 2 | human | 84984 | CEP19 | centrosomal protein 19 | XM_005269370.4 | 97.6% | 97.6% | 1_12del |
| 3 | human | 84984 | CEP19 | centrosomal protein 19 | XM_011513246.3 | 97.6% | 97.6% | 1_12del |
| 4 | mouse | 66994 | Cep19 | centrosomal protein 19 | NM_025892.2 | 83.4% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gtgcactgcc aagaaatgtg ggattaggtt tcagcctcca gctattatct 121 taatctatga gagtgaaatc aaggggaaaa ttcgccagcg cattatgcca gttcgaaact 181 tttcaaagtt ttcagattgc accagagctg ctgaacaatt aaagaataat ccgcgacaca 241 agagttacct agaacaagta tCCCTGAGGC AGCTAGAGAA GCTATTCAGT TTTTTACGAG 301 GTTACTTGTC GGGGCAGAGT CTGGCAGAAA CAATGGAACA AATTCAACGG GAAACAACCA 361 TTGATCCTGA GGAAGACCTG AACAAACTAG ATGACAAGGA GCTTGCCAAA AGAAAGAGCA 421 TCATGGATGA ACTTTTTGAG AAAAATCAGA AGAAGAAGGA TGATCCAAAT TTTGTTTATG 481 ACATTGAGGT TGAATTTCCA CAGGACGATC AACTGCAGTC CTGTGGCTGG GACACAGAGT 541 CAGCTGATGA GTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCTAAACCG TCTGAATTAA 721 TACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt