Construct: ORF TRCN0000466183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009773.1_s317c1
- Derived from:
- ccsbBroadEn_01474
- DNA Barcode:
- TGACACACAATCGTTTTGTGCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S100A4 (6275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6275 | S100A4 | S100 calcium binding protei... | NM_002961.3 | 100% | 100% | |
| 2 | human | 6275 | S100A4 | S100 calcium binding protei... | NM_019554.3 | 100% | 100% | |
| 3 | mouse | 20198 | S100a4 | S100 calcium binding protei... | NM_011311.2 | 89.8% | 93% | (many diffs) |
| 4 | mouse | 20198 | S100a4 | S100 calcium binding protei... | XM_006501177.3 | 89.8% | 93% | (many diffs) |
| 5 | mouse | 20198 | S100a4 | S100 calcium binding protei... | XM_006501178.2 | 89.8% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 393
- ORF length:
- 303
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGTGCCC TCTGGAGAAG GCCCTGGATG 121 TGATGGTGTC CACCTTCCAC AAGTACTCGG GCAAAGAGGG TGACAAGTTC AAGCTCAACA 181 AGTCAGAACT AAAGGAGCTG CTGACCCGGG AGCTGCCCAG CTTCTTGGGG AAAAGGACAG 241 ATGAAGCTGC TTTCCAGAAG CTGATGAGCA ACTTGGACAG CAACAGGGAC AACGAGGTGG 301 ACTTCCAAGA GTACTGTGTC TTCCTGTCCT GCATCGCCAT GATGTGTAAC GAATTCTTTG 361 AAGGCTTCCC AGATAAGCAG CCCAGGAAGA AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 541 ACACACAATC GTTTTGTGCC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt