Construct: ORF TRCN0000466242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000447.1_s317c1
- Derived from:
- ccsbBroadEn_03810
- DNA Barcode:
- TAGCCGCAGAATAACATTGTTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAGP (57228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57228 | SMAGP | small cell adhesion glycopr... | NM_001031628.2 | 100% | 100% | |
| 2 | human | 57228 | SMAGP | small cell adhesion glycopr... | NM_001033873.1 | 100% | 100% | |
| 3 | mouse | 207818 | Smagp | small cell adhesion glycopr... | NM_001033872.2 | 84.6% | 78.5% | (many diffs) |
| 4 | mouse | 207818 | Smagp | small cell adhesion glycopr... | NM_174992.4 | 84.6% | 78.5% | (many diffs) |
| 5 | mouse | 207818 | Smagp | small cell adhesion glycopr... | NM_001285413.1 | 74.4% | 69.3% | (many diffs) |
| 6 | mouse | 207818 | Smagp | small cell adhesion glycopr... | NM_001285414.1 | 74.4% | 69.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 384
- ORF length:
- 291
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGACCAG CCTCCTGACT ACTCCTTCTC 121 CAAGAGAAGA ACTGATGACC ACCCCAATTT TACAGCCCAC TGAGGCCCTG TCCCCAGAAG 181 ATGGAGCCAG CACAGCACTC ATTGCAGTTG TTATCACCGT TGTCTTCCTC ACCCTGCTCT 241 CGGTCGTGAT CTTGATCTTC TTTTACCTGT ACAAGAACAA AGGCAGCTAC GTCACCTATG 301 AACCTACAGA AGGTGAGCCC AGTGCCATCG TCCAGATGGA GAGTGACTTG GCCAAGGGCA 361 GCGAGAAAGA GGAATATTTC ATCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGCCGCAGAA 541 TAACATTGTT GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att