Construct: ORF TRCN0000466249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014329.1_s317c1
- Derived from:
- ccsbBroadEn_10899
- DNA Barcode:
- TATAGTCATACGTCTCGCGCGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDS (3423)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3423 | IDS | iduronate 2-sulfatase | NM_006123.5 | 89.2% | 82.4% | (many diffs) |
| 2 | human | 3423 | IDS | iduronate 2-sulfatase | NR_104128.2 | 62.9% | 1_169del;1106_1488del | |
| 3 | human | 3423 | IDS | iduronate 2-sulfatase | NM_000202.8 | 55.6% | 52.2% | (many diffs) |
| 4 | human | 3423 | IDS | iduronate 2-sulfatase | NM_001166550.4 | 39.3% | 35.9% | (many diffs) |
| 5 | mouse | 15931 | Ids | iduronate 2-sulfatase | NM_010498.3 | 47.9% | 46.1% | (many diffs) |
| 6 | mouse | 15931 | Ids | iduronate 2-sulfatase | XM_006527846.3 | 28.5% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1002
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gccaccccgg accggccgag gccttctctg gctgggtctg gttctgagct 121 ccgtctgcgt cgccctcgga tccgaaacgc aggccaactc gaccacagat gctctgaacg 181 ttcttctcat catcgtggat gacctgcgcc cctccctggg ctgttatggg gataagctgg 241 tgaggtcccc aaatattgac caactggcat cccacagcct cctcttccag aatgcctttg 301 cgcagcaagc agtgtgcgcc ccgagccgcg tttctttcct cactggcagg agacctgaca 361 ccacccgcct gtacgacttc aactcctact ggagggtgca cgctggaaac ttctccacca 421 tcccccagta cttcaaggag aatggctatg tgaccatgtc ggtgggaaaa gtctttcacc 481 ctgggatatc ttctaaccat accgatgatt ctccgtatag ctggtctttt ccaccttatc 541 atccttcctc tgagaagtat gaaaacacta agacatgtcg agggccagat ggagaactcc 601 atgccaacct gctttgccct gtggatgtgc tggatgttcc cgagggcacc ttgcctgaca 661 aacagagcac tgagcaagcc atacagttgt tggaaaagat gaaaacgtca gccagtcctt 721 tcttcctggc cgttgGGTAT CATAAGCCAC ACATCCCCTT CAGATACCCC AAGGAATTTC 781 AGAAGTTGTA TCCCTTGGAG AACATCACCC TGGCCCCCGA TCCCGAGGTC CCTGATGGCC 841 TACCCCCTGT GGCCTACAAC CCCTGGATGG ACATCAGGCA ACGGGAAGAC GTCCAAGCCT 901 TAAACATCAG TGTGCCGTAT GGTCCAATTC CTGTGGACTT TCAGGAGGAC CAAAGTTCCA 961 CAGGTTTCAG ACTGAAGACT TCATCTACCA GAAAGTATAA GTACCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATAT AGTCATACGT CTCGCGCGTC AACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t