Construct: ORF TRCN0000466267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004159.1_s317c1
- Derived from:
- ccsbBroadEn_01426
- DNA Barcode:
- GAATAATCCGTTCCGCGCGCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL23 (6150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | NM_021134.4 | 100% | 100% | |
| 2 | human | 107987373 | LOC107987373 | 39S ribosomal protein L23, ... | XM_006724896.2 | 99.7% | 99.3% | 364A>G |
| 3 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_006718271.2 | 75.3% | 64.1% | (many diffs) |
| 4 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_011520276.3 | 68.2% | 53.9% | (many diffs) |
| 5 | human | 107987373 | LOC107987373 | 39S ribosomal protein L23, ... | XM_017030111.1 | 68.2% | 53.9% | (many diffs) |
| 6 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_011520275.2 | 57.6% | 44.5% | (many diffs) |
| 7 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_011520273.1 | 56% | 43.3% | (many diffs) |
| 8 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | NM_011288.1 | 76.9% | 84.5% | (many diffs) |
| 9 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_006508517.2 | 74.4% | 81.2% | (many diffs) |
| 10 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_006508516.3 | 73.8% | 81.2% | (many diffs) |
| 11 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_017322050.1 | 66.5% | 69.3% | (many diffs) |
| 12 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_006508518.3 | 54.7% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggaatgtg gtgtaccccc tgtaccggct gggtggccca caacttcggg 121 tgttccgaac caacttcttc attcagctgg tgcggcccgg tgtggcccag cccgaggaca 181 ccgtgcagtt ccggatcccc atggaaatga caagggtgga cctcaggaaT TACCTCGAGG 241 GCATCTATAA CGTGCCCGTG GCTGCTGTGC GGACACGGGT GCAGCATGGC TCTAACAAGA 301 GAAGAGATCA CAGAAACGTG AGGATCAAGA AGCCGGACTA CAAGGTCGCC TACGTGCAGC 361 TGGCCCATGG ACAGACCTTC ACGTTCCCAG ATCTGTTTCC CGAGAAAGAC GAGAGCCCTG 421 AAGGCAGCGC TGCCGACGAC CTCTACAGCA TGCTCGAGGA GGAGAGGCAG CAGAGGCAGA 481 GCAGCGACCC GCGGCGGGGC GGCGTCCCCA GCTGGTTCGG GCTGTGCCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA GAATAATCCG TTCCGCGCGC TTATACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt