Construct: ORF TRCN0000466277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014225.1_s317c1
- Derived from:
- ccsbBroadEn_11943
- DNA Barcode:
- ACCACGTATTGTGGCGGGGTTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THAP4 (51078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51078 | THAP4 | THAP domain containing 4 | NM_001164356.1 | 31.7% | 30.5% | (many diffs) |
| 2 | human | 51078 | THAP4 | THAP domain containing 4 | NM_015963.6 | 29% | 28.5% | (many diffs) |
| 3 | human | 51078 | THAP4 | THAP domain containing 4 | XM_017004256.1 | 27.8% | 27.4% | (many diffs) |
| 4 | human | 51078 | THAP4 | THAP domain containing 4 | XM_011511291.2 | 26.6% | 26.2% | (many diffs) |
| 5 | human | 51078 | THAP4 | THAP domain containing 4 | XM_005247016.4 | 23.5% | 23.2% | (many diffs) |
| 6 | mouse | 67026 | Thap4 | THAP domain containing 4 | NM_001310761.1 | 28.7% | 28.5% | (many diffs) |
| 7 | mouse | 67026 | Thap4 | THAP domain containing 4 | NM_025920.3 | 26.4% | 26.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catcaacgag gtcatcctgt cggcgtcagg ggcctgcaag ctcatcgact 121 cactgcactc ctactgcttc tcctcccggc agaacaagag ccaggtgtgc tgcctgcggg 181 agcaggtgga gaagaagaac ggcgagctga agagccTGCG GCAGAGGGTC AGCCGCTCCG 241 ACAGCCAGGT GCGGAAGCTA CAGGAGAAGC TGGATGAGCT GAGGAGAGTG AGCGTCCCCT 301 ATCCAAGTAG CCTGCTGTCG CCCAGCCGCG AGCCCCCCAA GATGAACCCA GTGGTGGAGC 361 CACTGTCCTG GATGCTGGGC ACCTGGCTGT CGGACCCACC TGGAGCCGGG ACCTACCCCA 421 CACTGCAGCC CTTCCAGTAC CTGGAGGAGG TTCACATCTC CCACGTGGGC CAGCCCATGC 481 TGAACTTCTC GTTCAACTCC TTCCACCCGG ACACGCGCAA GCCGATGCAC AGAGAGTGTG 541 GCTTCATTCG CCTCAAGCCC GGAAGTTCAG GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 721 CACGTATTGT GGCGGGGTTC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt