Construct: ORF TRCN0000466312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002932.1_s317c1
- Derived from:
- ccsbBroadEn_03023
- DNA Barcode:
- GGGAGGACGGGTCATTGCGTAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRCP (27297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27297 | CRCP | CGRP receptor component | NM_001040647.1 | 100% | 100% | |
| 2 | human | 27297 | CRCP | CGRP receptor component | NM_001142414.1 | 81.3% | 80.8% | 1_78delinsA;81delT |
| 3 | human | 27297 | CRCP | CGRP receptor component | NM_014478.5 | 77.7% | 77.7% | 46_144del |
| 4 | human | 27297 | CRCP | CGRP receptor component | NM_001040648.1 | 61.4% | 59.1% | (many diffs) |
| 5 | human | 27297 | CRCP | CGRP receptor component | NR_024548.1 | 11.7% | 1_145del;191_411del;712_2925del | |
| 6 | mouse | 12909 | Crcp | calcitonin gene-related pep... | NM_007761.2 | 65.1% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 411
- ORF length:
- 345
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agtgaaggat gccaattctg cgcttctcag taactacgag acgttaaaat 121 acatatcaaa aacaccatgc aggcaccaga gtcctgaaat tgtcagagaa tttctcacag 181 cattgaaaag ccacaagttg acCAAAGCTG AGAAGCTCCA GCTGCTGAAC CACCGGCCTG 241 TGACTGCTGT GGAGATCCAG CTGATGGTGG AAGAGAGTGA AGAGCGGCTC ACGGAGGAGC 301 AGATTGAAGC TCTTCTCCAC ACCGTCACCA GCATTCTGCC TGCAGAGCCA GAGGCTGAGC 361 AGAAGAAGAA TACAAACAGC AATGTGGCAA TGGACGAAGA GGACCCAGCA TACCCAACTT 421 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 481 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 541 CTTGTGGAAA GGACGAGGGA GGACGGGTCA TTGCGTAGCG ACGCGTTAAG TCgacaatca 601 acctctggat tacaaaattt gtgaaagatt