Construct: ORF TRCN0000466320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012109.1_s317c1
- Derived from:
- ccsbBroadEn_12192
- DNA Barcode:
- CGGCAACGTTTCCTCTTTTCCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELP2 (55250)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001242879.3 | 99.8% | 99.7% | 382A>C;703G>A;1887C>T |
| 2 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001242877.3 | 94.3% | 94.2% | (many diffs) |
| 3 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_018255.4 | 91.4% | 91.2% | (many diffs) |
| 4 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001242878.3 | 88.8% | 88.7% | (many diffs) |
| 5 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001324466.2 | 87.2% | 87.1% | (many diffs) |
| 6 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001324468.2 | 86.5% | 81.2% | (many diffs) |
| 7 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001324465.2 | 86% | 85.9% | (many diffs) |
| 8 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001324467.2 | 85.3% | 85.2% | (many diffs) |
| 9 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001242875.3 | 84.7% | 84.6% | (many diffs) |
| 10 | human | 55250 | ELP2 | elongator acetyltransferase... | NM_001242876.3 | 82.1% | 82% | (many diffs) |
| 11 | human | 55250 | ELP2 | elongator acetyltransferase... | XR_430081.2 | 35.5% | (many diffs) | |
| 12 | human | 55250 | ELP2 | elongator acetyltransferase... | NR_136897.2 | 26.4% | (many diffs) | |
| 13 | human | 55250 | ELP2 | elongator acetyltransferase... | NR_040110.3 | 26.2% | (many diffs) | |
| 14 | human | 55250 | ELP2 | elongator acetyltransferase... | NR_136898.2 | 26.2% | (many diffs) | |
| 15 | human | 55250 | ELP2 | elongator acetyltransferase... | NR_137173.2 | 25.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2334
- ORF length:
- 2268
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcacccgtg ctggagactt ctcacgtgtt ttgctgccca aaccgggtgc 121 ggggagtcct gaactggagc tctgggccca gaggacttct ggcctttggc acgtcctgct 181 ccgtggtgct ctatgacccc ctgaaaaggg ttgttgttac caacttgaat ggtcacaccg 241 cccgagtcaa ttgcatacag tggatttgta aacaggatgg ctccccttct actgaattag 301 tttctggagg atctgataat caagtgattc actgggaaat agaggataat cagcttttaa 361 aagcagtgca tcttcaaggc catgaaggac ctgtttatgc ggtgcatgct gtttaccaga 421 ggaggacatc agatcctgca ttatgtccac tgatcgtttc tgcagctgca gattctgctg 481 ttcgactctg gtctaaaaag ggtccagaag gtagagatct tttcctagca agctgttcac 541 aagattgcct gataagaata tggaagctgt atataaagtc aacatcttta gaaactcagg 601 atgacgataa cataagactg aaagaaaata cttttaccat agaaaatgaa agtgttaaaa 661 tagcatttgc tgttactctg gagacagtgc tagccggtca tgaaaactgg gtaaatgcag 721 ttcactggca acctgtgttt tacaaagatg gtgtcctaca gcagccaatg agattattat 781 ctgcttccat ggataaaacc atgattctct gggctccaga tgaagagtca ggagtttggc 841 tagaacaggt tcgagtaggt gaagtaggtg ggaatacttt gggattttat gattgccagt 901 tcaatgaaga tggctccatg atcattgctc atgctttcca cggagcgttg cacctttgga 961 aacagaatac agttaaccca agagagtgga ctccagagat tgtcatttca ggacactttg 1021 atggtgtcca agacctagtc tgggatccag aaggagaatt tattatcact gttggtactg 1081 atcagacaac tagacttttt gctccatgga agagaaaaga ccaatcacag gtgacttggc 1141 atgaaattgc aaggcctcag atacatgggt atgacctgaa atgtttggca atgattaatc 1201 ggtttcagtt tgtatctgga gcagatgaaa aagttcttcg ggttttttct gcacctcgga 1261 attttgtgga aaatttttgt gccattacag gacaatcact gaatcatgtg ctctgtaatc 1321 aagatagtga tcttccagaa ggagccactg tccctgcatt gggattatca aataaagctg 1381 tctttcaggg agatatagct tctcagcctt ctgatgaaga ggagctgtta actagtactg 1441 gttttgagta tcagcaggtg gcctttcagc cctccatact tactgagcct cccactgagg 1501 atcatcttct gcagaatact ttgtggcctg aagttcaaaa actatatggg cacggttatg 1561 aaatattttg tgttacttgt aacagttcaa agactctgct tgcctcagct tgtaaggcag 1621 ctaagaaaga gcatgcagct atcattcttt ggaacactac atcttggaaa caggtgcaga 1681 atttagtttt ccacagtttg acagtcacgc agatggcctt ctcacctaat gagaagttct 1741 tactagctgt ttccagagat cgaacctggt cattgtggaa aaagcaggat acaatctcac 1801 ctgagttcga gccagttttt agtctttttg ccttcaccaa caaaattact tctgtgcaca 1861 gtagaattat ttggtcttgt gattggagtc ctgacagcaa gtatttcttc actgggagtc 1921 gagacaaaaa ggtggttgtc tggggtgagt gtgactccac tgatgactgt attgagcaca 1981 acattggccc ctgctccTCA GTCCTGGACG TGGGTGGGGC TGTGACAGCT GTCAGCGTCT 2041 GCCCAGTGCT CCACCCTTCT CAACGATACG TGGTTGCAGT AGGATTGGAG TGTGGAAAGA 2101 TTTGCTTATA TACCTGGAAA AAGACTGATC AAGTTCCAGA AATAAATGAC TGGACCCACT 2161 GTGTAGAAAC AAGTCAAAGC CAAAGTCATA CACTGGCTAT CAGAAAATTA TGCTGGAAGA 2221 ATTGCAGTGG AAAAACTGAA CAGAAGGAAG CAGAAGGTGC TGAGTGGTTA CACTTTGCAA 2281 GCTGTGGTGA AGATCACACT GTGAAGATAC ACAGAGTCAA TAAATGTGCA CTGTACCCAA 2341 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2401 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2461 TATCTTGTGG AAAGGACGAC GGCAACGTTT CCTCTTTTCC CATACGCGTT AAGTCgacaa 2521 tcaacctctg gattacaaaa tttgtgaaag att