Construct: ORF TRCN0000466332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018132.1_s317c1
- Derived from:
- ccsbBroadEn_07889
- DNA Barcode:
- TAGGTTGCAGCCCCCAGTGCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QPRT (23475)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23475 | QPRT | quinolinate phosphoribosylt... | NM_014298.5 | 99.8% | 99.6% | 157G>A |
| 2 | human | 23475 | QPRT | quinolinate phosphoribosylt... | XM_017023101.1 | 78.1% | 77.8% | 1_246del;403G>A;829A>G |
| 3 | human | 23475 | QPRT | quinolinate phosphoribosylt... | XM_005255223.3 | 77.9% | 77.6% | (many diffs) |
| 4 | human | 23475 | QPRT | quinolinate phosphoribosylt... | NM_001318250.1 | 50.1% | 50.1% | 104_105ins444 |
| 5 | human | 23475 | QPRT | quinolinate phosphoribosylt... | NM_001318249.1 | 47.8% | 39.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgctgaaggc ctggcgctgc tgctgccgcc cgtcaccctg gcagccctgg 121 tggacagctg gctccgagag gactgcccag ggctcaacta cgcagccttg gtcagcgggg 181 caggcccctc gcaggcggcg ctgtgggcca aatcccctgg gatactggca gggcagcctt 241 tcttcgatgc catatttacc caactcaact gccaagtctc ctggttcctc cccgagggat 301 cgaagctggt gccggtggcc agagtggccg aggtccgggg ccctgcccac tgcctgctgc 361 tgggggaacg ggtggccctc aacacgctgg cccgctgcag tggcattgcc agtgctgccg 421 ccgctgcagt ggaggccgcc aggggggccg gctggactgg gcacgtggca ggcacgagga 481 agaccacgcc aggcttccgg ctggtggaga agtatgggct cctggtgggc ggggccgcct 541 cgcaccgcta cgacctggga gggctggtga tggtgaagga taaccatgtg gtggccgccg 601 gtggcgtgga gaaggcggtg cgggcggcca gacaggcggc tgacttcgct ctgaaggtgg 661 aagtggaatg cagcagccTG CAGGAGGCCG TGCAGGCAGC TGAGGCTGGT GCCGACCTTG 721 TCCTGCTGGA CAACTTCAAG CCAGAGGAGC TGCACCCCAC GGCCACCGTG CTGAAGGCCC 781 AGTTCCCGAG TGTGGCTGTG GAAGCCAGTG GGGGCATCAC CCTGGACAAC CTCCCCCAGT 841 TCTGCGGGCC GCACATAGAC GTCATCTCCA TGGGGATGCT GACCCAGGCG GCCCCAGCCC 901 TTGATTTCTC CCTCAAGCTG TTTGCCAAAG AGGTGGCTCC AGTGCCCAAA ATCCACTACC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATAGGTTGC AGCCCCCAGT GCCACGACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt