Construct: ORF TRCN0000466355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015920.1_s317c1
- Derived from:
- ccsbBroadEn_10354
- DNA Barcode:
- TTCTAACCCCTTTGTAGACCAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BP1 (337875)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 51.8% | 51.5% | 1_177del;279C>T;378_378delGinsCATTTTC |
| 2 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 51.3% | 51.5% | (many diffs) |
| 3 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 50.9% | 49.2% | (many diffs) |
| 4 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 48.4% | 51.5% | (many diffs) |
| 5 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 48.4% | 51.5% | (many diffs) |
| 6 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 48.4% | 50.7% | (many diffs) |
| 7 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 48.4% | 50.7% | (many diffs) |
| 8 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 48.4% | 50% | (many diffs) |
| 9 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 47.9% | 51.5% | (many diffs) |
| 10 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 47.9% | 50% | (many diffs) |
| 11 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 47.9% | 50% | (many diffs) |
| 12 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 47.6% | 51.5% | (many diffs) |
| 13 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 47.6% | 51.5% | (many diffs) |
| 14 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 47.6% | 51.5% | (many diffs) |
| 15 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 47.6% | 50.7% | (many diffs) |
| 16 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 47.3% | 51.5% | (many diffs) |
| 17 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 46.8% | 51.5% | (many diffs) |
| 18 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 46.6% | 51.5% | (many diffs) |
| 19 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 46.6% | 51.5% | (many diffs) |
| 20 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 46.6% | 50.7% | (many diffs) |
| 21 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 46% | 51.5% | (many diffs) |
| 22 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 45.8% | 51.5% | (many diffs) |
| 23 | human | 255626 | H2BC1 | H2B clustered histone 1 | NM_170610.2 | 42.1% | 47.2% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 41.6% | (many diffs) | |
| 25 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 40.1% | 43.4% | (many diffs) |
| 26 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 33.7% | (many diffs) | |
| 27 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 12.5% | (many diffs) | |
| 28 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 11.3% | 1_227del;366A>G;435_1822del | |
| 29 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 10.2% | (many diffs) | |
| 30 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 9.5% | (many diffs) | |
| 31 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 9.3% | 1_597del;736A>G;805_2192del | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 8.3% | (many diffs) | |
| 33 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 6.7% | (many diffs) | |
| 34 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 49.2% | 51.5% | (many diffs) |
| 35 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 49.2% | 51.5% | (many diffs) |
| 36 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 49.2% | 51.5% | (many diffs) |
| 37 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 49.2% | 51.5% | (many diffs) |
| 38 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 49.2% | 50.7% | (many diffs) |
| 39 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 48.9% | 51.5% | (many diffs) |
| 40 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 48.9% | 51.5% | (many diffs) |
| 41 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 48.9% | 51.5% | (many diffs) |
| 42 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 48.6% | 51.5% | (many diffs) |
| 43 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 48.6% | 50.7% | (many diffs) |
| 44 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 48.6% | 50.7% | (many diffs) |
| 45 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 48.6% | 50.7% | (many diffs) |
| 46 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 48.6% | 50.7% | (many diffs) |
| 47 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 48.6% | 50.7% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 48.6% | 50.7% | (many diffs) |
| 49 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 48.4% | 50.7% | (many diffs) |
| 50 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 48.4% | 50.7% | (many diffs) |
| 51 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 48.1% | 50% | (many diffs) |
| 52 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 47.6% | 50% | (many diffs) |
| 53 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 47.6% | 49.2% | (many diffs) |
| 54 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 47.6% | 49.2% | (many diffs) |
| 55 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 47.5% | 48.8% | (many diffs) |
| 56 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 46.6% | 47.8% | (many diffs) |
| 57 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 45.8% | 51.5% | (many diffs) |
| 58 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 45% | 49.2% | (many diffs) |
| 59 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 182
- ORF end:
- 389
- ORF length:
- 207
- Sequence:
-
1 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT 61 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 121 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAC 181 CATGGGCATC ATGAACTCCT TCGTCAACGA CATCTTCGAG CGCATCGCGG GAGAGGCGTC 241 CCGCCTGGCG CACTACAACA AGCGCTCCAC CATCACATCC CGTGAGATCC AGACGGCCGT 301 GCGCCTGCTG CTGCCCGGCG AGCTGGCCAA GCACGCCGTG TCCGAGGGCA CCAAGGCGGT 361 CACCAAGTAC ACCAGCTCGA ACATTTTCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT 421 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 481 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCTAA 541 CCCCTTTGTA GACCAATGAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 601 gaaagatt