Construct: ORF TRCN0000466363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010977.2_s317c1
- Derived from:
- ccsbBroadEn_15358
- DNA Barcode:
- CCTGGGTTTACAGATGTGGCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARAF (369)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001654.5 | 100% | 100% | |
| 2 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001256196.1 | 99.5% | 99.5% | 459_467delGCCCTCAAG |
| 3 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001256197.1 | 30.6% | 30.5% | 558_558delGins1261 |
| 4 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | NM_009703.2 | 89.9% | 95.2% | (many diffs) |
| 5 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_006527556.3 | 89.9% | 95.2% | (many diffs) |
| 6 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_006527557.3 | 89.9% | 95.2% | (many diffs) |
| 7 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_011247429.2 | 84.8% | 89.7% | (many diffs) |
| 8 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_011247430.2 | 67.1% | 69.1% | (many diffs) |
| 9 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | NM_001159645.1 | 27.7% | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1884
- ORF length:
- 1818
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccaccacgg ggcccccctg ccaatggggc cgagccatcc cgggcagtgg 121 gcaccgtcaa agtatacctg cccaacaagc aacgcacggt ggtgactgtc cgggatggca 181 tgagtgtcta cgactctcta gacaaggccc tgaaggtgcg gggtctaaat caggactgct 241 gtgtggtcta ccgactcatc aagggacgaa agacggtcac tgcctgggac acagccattg 301 ctcccctgga tggcgaggag ctcattgtcg aggtccttga agatgtcccg ctgaccatgc 361 acaattttgt acggaagacc ttcttcagcc tggcgttctg tgacttctgc cttaagtttc 421 tgttccatgg cttccgttgc caaacctgtg gctacaagtt ccaccagcat tgttcctcca 481 aggtccccac agtctgtgtt gacatgagta ccaaccgcca acagttctac cacagtgtcc 541 aggatttgtc cggaggctcc agacagcatg aggctccctc gaaccgcccc ctgaatgagt 601 tgctaacccc ccagggtccc agcccccgca cccagcactg tgacccggag cacttcccct 661 tccctgcccc agccaatgcc cccctacagc gcatccgctc cacgtccact cccaacgtcc 721 atatggtcag caccacggcc cccatggact ccaacctcat ccagctcact ggccagagtt 781 tcagcactga tgctgccggt agtagaggag gtagtgatgg aaccccccgg gggagcccca 841 gcccagccag cgtgtcctcg gggaggaagt ccccacattc caagtcacca gcagagcagc 901 gcgagcggaa gtccttggcc gatgacaaga agaaagtgaa gaacctgggg taccgggact 961 caggctatta ctgggaggta ccacccagtg aggtgcagct gctgaagagg atcgggacgg 1021 gctcgtttgg caccgtgttt cgagggcggt ggcatggcga tgtggccgtg aaggtgctca 1081 aggtgtccca gcccacagct gagcaggccc aggctttcaa gaatgagatg caggtgctca 1141 ggaagacgcg acatgtcaac atcttgctgt ttatgggctt catgacccgg ccgggatttg 1201 ccatcatcac acagtggtgt gagggctcca gcctctacca tcacctgcat gtggccgaca 1261 cacgcttcga catggtccag ctcatcgacg tggcccggca gactgcccag ggcatggact 1321 acctccatgc caagaacatc atccaccgag atctcaagtc taacaacatc ttcctacatg 1381 aggggctcac ggtgaagatc ggtgactttg gcttggccac agtgaagact cgatggagcg 1441 gggcccagcc cttggagcag ccctcaggat ctgtgctgtg gatggcagct gaggtgatcc 1501 gtatgcagga cccgaacccc tacagcttcc agtcagacgt ctatgcctac ggggttgtgc 1561 tctacgagct tatgactggc tcactgcctt acagccacat tggctgccgt gaccagatta 1621 tctttatggt gggccgtggc tatctgtccc cggaccTCAG CAAAATCTCC AGCAACTGCC 1681 CCAAGGCCAT GCGGCGCCTG CTGTCTGACT GCCTCAAGTT CCAGCGGGAG GAGCGGCCCC 1741 TCTTCCCCCA GATCCTGGCC ACAATTGAGC TGCTGCAACG GTCACTCCCC AAGATTGAGC 1801 GGAGTGCCTC GGAACCCTCC TTGCACCGCA CCCAGGCCGA TGAGTTGCCT GCCTGCCTAC 1861 TCAGCGCAGC CCGCCTTGTG CCTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1921 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1981 GTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTGGGTTTAC 2041 AGATGTGGCT TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2101 att