Construct: ORF TRCN0000466386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003689.1_s317c1
- Derived from:
- ccsbBroadEn_09973
- DNA Barcode:
- TGGGCATATTGCTTGCTGACCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM126B (285172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285172 | FAM126B | family with sequence simila... | NM_001321618.1 | 99.9% | 100% | 99G>A |
| 2 | human | 285172 | FAM126B | family with sequence simila... | NM_001321619.2 | 99.9% | 100% | 99G>A |
| 3 | human | 285172 | FAM126B | family with sequence simila... | NM_001321621.2 | 99.9% | 100% | 99G>A |
| 4 | human | 285172 | FAM126B | family with sequence simila... | NM_001321622.2 | 99.9% | 100% | 99G>A |
| 5 | human | 285172 | FAM126B | family with sequence simila... | NM_173822.5 | 99.9% | 100% | 99G>A |
| 6 | human | 285172 | FAM126B | family with sequence simila... | XM_017003881.1 | 99.9% | 100% | 99G>A |
| 7 | human | 285172 | FAM126B | family with sequence simila... | NM_001321623.1 | 90.3% | 90.4% | 99G>A;991_1158del |
| 8 | human | 285172 | FAM126B | family with sequence simila... | NM_001321624.1 | 90.3% | 90.4% | 99G>A;991_1158del |
| 9 | human | 285172 | FAM126B | family with sequence simila... | NM_001321625.2 | 90.3% | 90.4% | 99G>A;991_1158del |
| 10 | human | 285172 | FAM126B | family with sequence simila... | NM_001321626.2 | 90.3% | 90.4% | 99G>A;991_1158del |
| 11 | human | 285172 | FAM126B | family with sequence simila... | XM_006712443.3 | 90.3% | 90.4% | 99G>A;991_1158del |
| 12 | human | 285172 | FAM126B | family with sequence simila... | XM_011511002.2 | 90.3% | 90.4% | 99G>A;991_1158del |
| 13 | human | 285172 | FAM126B | family with sequence simila... | XM_017003879.1 | 90.3% | 90.4% | 99G>A;991_1158del |
| 14 | human | 285172 | FAM126B | family with sequence simila... | XM_017003880.1 | 90.3% | 90.4% | 99G>A;991_1158del |
| 15 | human | 285172 | FAM126B | family with sequence simila... | NM_001321629.1 | 84.5% | 84.5% | 0_1ins246 |
| 16 | human | 285172 | FAM126B | family with sequence simila... | XM_017003883.1 | 84.5% | 84.5% | 0_1ins246 |
| 17 | human | 285172 | FAM126B | family with sequence simila... | NM_001321627.1 | 76.4% | 76.4% | 0_1ins246;745_912del |
| 18 | human | 285172 | FAM126B | family with sequence simila... | NM_001321628.2 | 76.4% | 76.4% | 0_1ins246;745_912del |
| 19 | human | 285172 | FAM126B | family with sequence simila... | XM_017003882.1 | 76.4% | 76.4% | 0_1ins246;745_912del |
| 20 | mouse | 213056 | Fam126b | family with sequence simila... | NM_001310599.1 | 91.7% | 95.2% | (many diffs) |
| 21 | mouse | 213056 | Fam126b | family with sequence simila... | NM_172513.3 | 91.7% | 95.2% | (many diffs) |
| 22 | mouse | 213056 | Fam126b | family with sequence simila... | XM_017319657.1 | 91.7% | 95.2% | (many diffs) |
| 23 | mouse | 213056 | Fam126b | family with sequence simila... | NM_001310598.1 | 82.9% | 86.1% | (many diffs) |
| 24 | mouse | 213056 | Fam126b | family with sequence simila... | XM_006495832.2 | 82.9% | 86.1% | (many diffs) |
| 25 | mouse | 213056 | Fam126b | family with sequence simila... | XM_006495833.2 | 82.9% | 86.1% | (many diffs) |
| 26 | mouse | 213056 | Fam126b | family with sequence simila... | XM_017319658.1 | 67% | 69% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggaactgac cgttgtgttg tggaagaatg gttatcagaa ttcaaggcat 121 tacctgacac tcagatcacc agttatgcag caactttaca ccgaaaaaaa acacttgtac 181 cagccctcta taaagttatt caagattcaa ataatgagct cctggagcct gtctgccatc 241 agctgtttga gctctatcgt agctcagagg ttcgacttaa gaggttcaca ctgcagttct 301 tgccagaatt gatgtgggtt tatttacggc ttacagttag ccgagacaga cagagtaatg 361 gttgcattga agcacttctg ttaggaattt acaatttgga aatcgctgat aaagatggga 421 acaataaagt tctgtctttc actatcccct ccttatccaa gccttcaata taccatgaac 481 cttcaacaat tggatccatg gctttgacag aaggggcatt gtgtcagcat gatctcatca 541 gagttgttta tagtgatctt catcctcaga gggaaacatt cactgcacag aaccggtttg 601 aagtcctgag ttttctcatg ctgtgttata attctgctat tgtatatatg cctgcctcat 661 cttaccaatc tctttgtcgg atgggttcca gggtttgtgt gagtggcttt ccacggcaac 721 atgaaaaaca ctggaaagaa ctctgtggtc gaatagtatt ggatcctgaa tttatggtgc 781 aacttctcac aggggtttat tatgccatgt ataatggaca gtgggacctt ggccaggaag 841 ttcttgatga tatcatttat agagcccagc tagagctttt ttctcaacca ctattggttg 901 ccaatgccat gaaaaactca ttaccatttg atgctcctga ttctacacaa gaaggccaga 961 aagtccttaa agttgaagtc actccaacag tgccgaggat ttctcggact gcaattacaa 1021 cagcttcaat ccgtcgtcat agatggagaa gagaaggtgc tgagggtgta aatggaggag 1081 aggagtctgt aaacctgaat gatgcagatg aaggattttc atcaggggct tccctcagca 1141 gtcagccaat tgggaccaaa ccatcctcct cttctcagag gggaagctta aggaaagtag 1201 caactgggcg ttcagccaag gataaagaaa cagccTCTGC CATCAAATCC AGTGAGAGCC 1261 CTCGAGATTC AGTAGTTCGC AAGCAGTATG TACAGCAACC AACTGATCTT AGTGTAGATT 1321 CAGTTGAGCT GACACCAATG AAGAAACACC TGAGCCTGCC TGCTGGCCAG GTGGTGCCAA 1381 AAATCAATAG CTTAAGTCTA ATCCGGACAG CCAGTGCTTC CTCAAGTAAA TCATTTGACT 1441 ATGTAAATGG CAGTCAAGCA AGTACCAGCA TTGGGGTTGG CACTGAGGGA GGTACTAATT 1501 TAGCAGCCAA CAATGCTAAT CGATACTCAA CTGTCAGTCT GCAGGAAGAC CGGCTAGGTC 1561 AAGCTGGCGA AGGTAAAGAG CTCCTCAGCC CAGGAGCCCC CTTGACCAAG CAGTCTCGAT 1621 CCCCAAGTTT CAATATGCAG CTAATATCCC AGGTGTACCC AACTTTCTTG TACAAAGTGG 1681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1801 ATGGGCATAT TGCTTGCTGA CCTTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1861 aatttgtgaa agatt