Construct: ORF TRCN0000466397

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013296.1_s317c1
Derived from:
ccsbBroadEn_13733
DNA Barcode:
GCGGGGGCGTGTGCCAGAGAATTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GUSBP14 (11039)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466397
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 375513 GUSBP4 GUSB pseudogene 4 NR_133000.1 51.8% (many diffs)
2 human 100049076 GUSBP9 GUSB pseudogene 9 NR_033968.1 43.7% (many diffs)
3 human 106660613 LINC00680-GUSBP4 LINC00680-GUSBP4 readthrough NR_132998.1 39.4% (many diffs)
4 human 106660613 LINC00680-GUSBP4 LINC00680-GUSBP4 readthrough NR_003660.2 35.7% (many diffs)
5 human 728411 GUSBP1 GUSB pseudogene 1 NR_027028.2 35.5% (many diffs)
6 human 102725009 LOC102725009 putative inactive beta-gluc... XM_017030249.2 33.2% 13.1% 1_268del;450_451ins97
7 human 11039 GUSBP14 GUSB pseudogene 14 NR_024054.2 27.4% 1_189del;469_1016del
8 human 106660613 LINC00680-GUSBP4 LINC00680-GUSBP4 readthrough NR_132997.1 26.7% (many diffs)
9 human 387036 GUSBP2 GUSB pseudogene 2 NR_003504.3 23% (many diffs)
10 human 2990 GUSB glucuronidase beta XM_017012093.2 22.6% 16.2% (many diffs)
11 human 2990 GUSB glucuronidase beta XM_017012092.1 20.7% 14.8% (many diffs)
12 human 2990 GUSB glucuronidase beta XM_011516114.2 19.9% 14.2% (many diffs)
13 human 2990 GUSB glucuronidase beta NM_001293105.2 19.6% 14.1% (many diffs)
14 human 2990 GUSB glucuronidase beta XM_017012091.1 19.6% 14% (many diffs)
15 human 2990 GUSB glucuronidase beta NM_001293104.2 18.4% 13.2% (many diffs)
16 human 653188 GUSBP3 GUSB pseudogene 3 NR_027386.2 17.7% (many diffs)
17 human 2990 GUSB glucuronidase beta XM_011516113.1 17.5% 12.6% (many diffs)
18 human 2990 GUSB glucuronidase beta NM_001284290.2 16.8% 12% (many diffs)
19 human 11042 GUSBP15 GUSB pseudogene 15 NR_034021.1 15.4% (many diffs)
20 human 11039 GUSBP14 GUSB pseudogene 14 NR_029426.1 14.3% 1_481del;761_1943del
21 human 2990 GUSB glucuronidase beta XM_005250297.4 14.1% 10.1% (many diffs)
22 human 2990 GUSB glucuronidase beta NM_000181.4 13% 9.3% (many diffs)
23 human 2990 GUSB glucuronidase beta XR_001744660.2 12.6% (many diffs)
24 human 2990 GUSB glucuronidase beta XR_927461.3 11.7% (many diffs)
25 human 653080 GUSBP16 GUSB pseudogene 16 NR_146391.1 11.6% (many diffs)
26 human 11039 GUSBP14 GUSB pseudogene 14 XR_002958912.1 5.5% 1_846del;1126_4984del
27 human 375513 GUSBP4 GUSB pseudogene 4 NR_132999.1 5.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
345
ORF length:
279
Sequence:
1tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG
61TTGGCATGGT GATTGCTCAC ACCAAAGCCT TGGACCCCTC CCAGCCTGTG ACCTTTGTGA
121CCAACTCCAC CTACGCAGCA GACAAGGGGG CTCTGTATGT GGATGTGATC CGTGTGAACA
181GCTACTACTC TTGGTATCGC AACTACGGGC ACCTGGAGTT GATTCAGCTG CAGCTGGCCG
241CCCAGTTTGA GAATTGGTGT AAGACATCAC AATCCCATTA TTCAGAGCGC GTATGGAGTG
301GAAACGCTTG TAGGGTTTCA CCAGGATCCA CCTCTGATGT TCAGTGCCCA ACTTTCTTGT
361ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
421AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
481GAAAGGACGA GCGGGGGCGT GTGCCAGAGA ATTCACGCGT TAAGTCgaca atcaacctct
541ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)