Construct: ORF TRCN0000466397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013296.1_s317c1
- Derived from:
- ccsbBroadEn_13733
- DNA Barcode:
- GCGGGGGCGTGTGCCAGAGAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUSBP14 (11039)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466397
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_133000.1 | 51.8% | (many diffs) | |
| 2 | human | 100049076 | GUSBP9 | GUSB pseudogene 9 | NR_033968.1 | 43.7% | (many diffs) | |
| 3 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_132998.1 | 39.4% | (many diffs) | |
| 4 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_003660.2 | 35.7% | (many diffs) | |
| 5 | human | 728411 | GUSBP1 | GUSB pseudogene 1 | NR_027028.2 | 35.5% | (many diffs) | |
| 6 | human | 102725009 | LOC102725009 | putative inactive beta-gluc... | XM_017030249.2 | 33.2% | 13.1% | 1_268del;450_451ins97 |
| 7 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_024054.2 | 27.4% | 1_189del;469_1016del | |
| 8 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_132997.1 | 26.7% | (many diffs) | |
| 9 | human | 387036 | GUSBP2 | GUSB pseudogene 2 | NR_003504.3 | 23% | (many diffs) | |
| 10 | human | 2990 | GUSB | glucuronidase beta | XM_017012093.2 | 22.6% | 16.2% | (many diffs) |
| 11 | human | 2990 | GUSB | glucuronidase beta | XM_017012092.1 | 20.7% | 14.8% | (many diffs) |
| 12 | human | 2990 | GUSB | glucuronidase beta | XM_011516114.2 | 19.9% | 14.2% | (many diffs) |
| 13 | human | 2990 | GUSB | glucuronidase beta | NM_001293105.2 | 19.6% | 14.1% | (many diffs) |
| 14 | human | 2990 | GUSB | glucuronidase beta | XM_017012091.1 | 19.6% | 14% | (many diffs) |
| 15 | human | 2990 | GUSB | glucuronidase beta | NM_001293104.2 | 18.4% | 13.2% | (many diffs) |
| 16 | human | 653188 | GUSBP3 | GUSB pseudogene 3 | NR_027386.2 | 17.7% | (many diffs) | |
| 17 | human | 2990 | GUSB | glucuronidase beta | XM_011516113.1 | 17.5% | 12.6% | (many diffs) |
| 18 | human | 2990 | GUSB | glucuronidase beta | NM_001284290.2 | 16.8% | 12% | (many diffs) |
| 19 | human | 11042 | GUSBP15 | GUSB pseudogene 15 | NR_034021.1 | 15.4% | (many diffs) | |
| 20 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_029426.1 | 14.3% | 1_481del;761_1943del | |
| 21 | human | 2990 | GUSB | glucuronidase beta | XM_005250297.4 | 14.1% | 10.1% | (many diffs) |
| 22 | human | 2990 | GUSB | glucuronidase beta | NM_000181.4 | 13% | 9.3% | (many diffs) |
| 23 | human | 2990 | GUSB | glucuronidase beta | XR_001744660.2 | 12.6% | (many diffs) | |
| 24 | human | 2990 | GUSB | glucuronidase beta | XR_927461.3 | 11.7% | (many diffs) | |
| 25 | human | 653080 | GUSBP16 | GUSB pseudogene 16 | NR_146391.1 | 11.6% | (many diffs) | |
| 26 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | XR_002958912.1 | 5.5% | 1_846del;1126_4984del | |
| 27 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_132999.1 | 5.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 345
- ORF length:
- 279
- Sequence:
-
1 tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGT GATTGCTCAC ACCAAAGCCT TGGACCCCTC CCAGCCTGTG ACCTTTGTGA 121 CCAACTCCAC CTACGCAGCA GACAAGGGGG CTCTGTATGT GGATGTGATC CGTGTGAACA 181 GCTACTACTC TTGGTATCGC AACTACGGGC ACCTGGAGTT GATTCAGCTG CAGCTGGCCG 241 CCCAGTTTGA GAATTGGTGT AAGACATCAC AATCCCATTA TTCAGAGCGC GTATGGAGTG 301 GAAACGCTTG TAGGGTTTCA CCAGGATCCA CCTCTGATGT TCAGTGCCCA ACTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA GCGGGGGCGT GTGCCAGAGA ATTCACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt