Construct: ORF TRCN0000466401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005556.1_s317c1
- Derived from:
- ccsbBroadEn_08234
- DNA Barcode:
- TCATGCTGCGCCGTGGGACTGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRTO4 (51154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51154 | MRTO4 | MRT4 homolog, ribosome matu... | NM_016183.4 | 99.8% | 100% | 330G>A |
| 2 | human | 51154 | MRTO4 | MRT4 homolog, ribosome matu... | XM_006710675.4 | 80.1% | 80.3% | 0_1ins141;189G>A |
| 3 | mouse | 69902 | Mrto4 | mRNA turnover 4, ribosome m... | NM_001290810.1 | 88.8% | 93.3% | (many diffs) |
| 4 | mouse | 69902 | Mrto4 | mRNA turnover 4, ribosome m... | NM_023536.3 | 88.4% | 92.8% | (many diffs) |
| 5 | mouse | 69902 | Mrto4 | mRNA turnover 4, ribosome m... | XM_006539162.2 | 70.2% | 73.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caaatccaag cgcgacaaga aagtctcctt aaccaaaact gccaagaaag 121 gcttggaatt gaaacaaaac ctgatagaag agcttcggaa atgtgtggac acctacaagt 181 accttttcat cttctctgtg gccaacatga ggaacagcaa gctgaaggac atccggaacg 241 cctggaagca cagccggatg ttctttggca aaaacaaggt gatgatggtg gccttgggtc 301 ggagcccatc tgatgaatac aaagacaacc tgcaccaggt cagcaaaagg ttgaggggtg 361 aggtgggtct cctgttcacc aaccgcacaa aggaagaggt gaatgagtgg ttcacgaaat 421 acacagaaat ggactacgcc cgagctggta acaaagcagc tttcactgtg agcctggatc 481 cagggcccct ggagcagttc ccccactcca tggagccaca gctcaggcag ctgggcctgc 541 ccaccgccct caagagaggt gtggtgactc TGCTGTCTGA CTACGAGGTG TGCAAGGAGG 601 GCGATGTGCT GACCCCAGAG CAGGCTCGCG TCCTGAAGCT TTTTGGGTAT GAGATGGCTG 661 AATTCAAGGT GACCATCAAA TACATGTGGG ATTCACAGTC GGGAAGGTTC CAGCAGATGG 721 GAGACGACTT GCCAGAGAGC GCATCTGAGT CCACAGAAGA GTCAGACTCA GAAGATGATG 781 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGATC ATGCTGCGCC GTGGGACTGT GAACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt