Construct: ORF TRCN0000466414
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007978.1_s317c1
- Derived from:
- ccsbBroadEn_03492
- DNA Barcode:
- ACACGCGACTGCCCACTGGCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPCAT2 (54947)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466414
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54947 | LPCAT2 | lysophosphatidylcholine acy... | NM_017839.5 | 100% | 100% | |
| 2 | human | 54947 | LPCAT2 | lysophosphatidylcholine acy... | XM_017023376.1 | 89.5% | 89.5% | 0_1ins169;2delT |
| 3 | human | 54947 | LPCAT2 | lysophosphatidylcholine acy... | XM_005256006.3 | 56.7% | 53.5% | (many diffs) |
| 4 | human | 54947 | LPCAT2 | lysophosphatidylcholine acy... | XM_011523169.3 | 50.3% | 50.3% | 0_1ins810 |
| 5 | mouse | 270084 | Lpcat2 | lysophosphatidylcholine acy... | NM_173014.1 | 84.6% | 88.4% | (many diffs) |
| 6 | mouse | 270084 | Lpcat2 | lysophosphatidylcholine acy... | XM_006531051.2 | 74.8% | 78.3% | (many diffs) |
| 7 | mouse | 270084 | Lpcat2 | lysophosphatidylcholine acy... | XM_006531052.2 | 48.9% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1698
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccggtgcgcc caggcggcgg aagtggcggc cacagtgcca ggtgccggcg 121 tcgggaacgt ggggctgcgg ccgcccatgg tgccccgtca ggcgtccttc ttcccgccgc 181 cggtgccgaa ccccttcgtg cagcagacgc agatcggctc cgcgaggcgg gtccagattg 241 tccttcttgg gattatcttg cttccaattc gtgtcttatt ggttgcgtta attttattac 301 ttgcatggcc atttgctgca atttcaacag tatgctgtcc tgaaaagctg acccacccaa 361 taactggttg gaggaggaaa attactcaaa cagctttgaa atttctgggt cgtgctatgt 421 tcttttcaat gggatttata gttgctgtaa aaggaaagat tgcaagtcct ttggaagcac 481 cagtttttgt tgctgcccct cattcaacat tctttgatgg aattgcctgt gttgtagctg 541 ggttaccttc tatggtatct cgaaatgaga atgcacaagt ccctctgatt ggcagactgt 601 tacgggctgt gcaaccagtt ttggtgtccc gtgtagatcc ggattcccga aaaaacacaa 661 taaatgaaat aataaagcga acaacatcag gaggagaatg gccccagata ctagttttcc 721 cagaaggtac ttgtactaat cgttcctgtt tgattacttt taaaccagga gccttcattc 781 caggagttcc agtgcagcca gtcctcctca gatacccaaa caagctggat actgtgacct 841 ggacatggca aggatataca ttcattcagc tttgtatgct tactttctgc cagctcttca 901 caaaggtaga agttgagttt atgccagttc aagtaccaaa tgatgaagaa aaaaatgatc 961 ctgtcctttt tgccaataaa gtccggaatt taatggcaga agctctggga ataccagtaa 1021 cagatcatac ctatgaagac tgcagattga tgatttcagc aggacagcta acattgccta 1081 tggaagctgg gctggtggaa tttactaaaa ttagccgaaa attgaaatta gattgggatg 1141 gtgttcgtaa gcatttggat gaatatgcat ctattgcgag ttcctcaaaa ggaggaagaa 1201 ttggaattga agaattcgcc aagtatttaa agttgcctgt ttcagatgtc ttgagacaac 1261 tttttgcact ctttgacagg aaccatgatg gcagcattga cttccgagag tatgtgattg 1321 gcctggctgt cttgtgcaac ccttccaaca cagaggagat catccaggtg gcatttaagc 1381 tgtttgacgt tgatgaggat ggctacataa cggaggaaga gttctccacc attctacagg 1441 cttcccttgg agtgcctgac cttgatgttt ctggtctctt caaggaaata gcccaagggg 1501 actcaatttc ctatgaggaa tttaaaagtt ttgccTTAAA GCATCCAGAA TATGCTAAGA 1561 TATTTACAAC ATACCTAGAC CTCCAGACGT GCCATGTGTT TTCATTACCA AAAGAAGTCC 1621 AGACAACCCC CTCCACCGCC AGTAATAAAG TCAGCCCTGA AAAGCATGAA GAGAGTACCT 1681 CAGACAAAAA AGATGACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1741 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1801 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACACGCG ACTGCCCACT 1861 GGCATTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt