Construct: ORF TRCN0000466430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015030.1_s317c1
- Derived from:
- ccsbBroadEn_01293
- DNA Barcode:
- AGCAGCAGATACCCGAAGAAGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC3 (5611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | NM_006260.5 | 100% | 100% | |
| 2 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | XM_011521104.3 | 94.5% | 94.5% | 393_479del |
| 3 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | XM_017020674.2 | 83.5% | 83.5% | 0_1ins249 |
| 4 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | XM_011521105.2 | 78.9% | 78.9% | 0_1ins249;144_230del |
| 5 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | XM_017020675.2 | 75.5% | 73.9% | (many diffs) |
| 6 | mouse | 100037258 | Dnajc3 | DnaJ heat shock protein fam... | NM_008929.3 | 87.5% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcccccggc tccgtgacca gccggctggg ctcggtattc cccttcctgc 121 tagtcctggt ggatctgcag tacgaaggtg ctgaatgtgg agtaaatgca gatgttgaga 181 aacatcttga attgggcaag aaattacttg cagctggaca gctagctgat gctttatctc 241 agtttcatgc tgccgtagat ggtgaccctg ataactatat tgcttattat cggagggcta 301 ctgtcttttt agctatgggc aaatcaaaag ctgcacttcc tgatttaact aaagtgattc 361 aattgaagat ggacttcact gcagcaagat tacagagagg tcacttatta ctcaaacaag 421 gaaaacttga tgaagcagaa gatgatttta aaaaagtgct caaatctaat ccaagtgaaa 481 atgaagaaaa ggaagcacag tctcaactta taaaatctga tgaaatgcag cgtttgcgtt 541 cacaagcact taacgctttt ggaagtggag attatactgc tgctatagcc ttccttgata 601 agattttaga ggtttgtgtt tgggatgcag aactacggga acttcgagct gaatgtttta 661 taaaagaagg agaacctagg aaagctataa gtgacttaaa agctgcgtca aagttgaaga 721 atgataatac tgaagcgttt tataaaataa gcacactgta ctaccaacta ggagaccacg 781 aactgtccct cagtgaagtt cgggaatgtc ttaaacttga ccaggatcat aaaaggtgtt 841 ttgcacacta taaacaagta aagaaactta ataagctgat tgagtcagct gaagagctca 901 tcagagatgg cagatacaca gatgctacca gcaaatatga atctgtcatg aaaacagagc 961 caagcattgc tgaatataca gttcgttcaa aggagaggat ttgccactgc ttttctaagg 1021 acgagaagcc tgttgaagct attagggttt gttctgaagt tttacagatg gaacctgaca 1081 atgtgaatgc cctgaaagat cgagcagagg cctatttgat agaggaaatg tatgatgaag 1141 ctattCAGGA TTATGAAACT GCTCAGGAAC ACAATGAAAA TGATCAGCAG ATTCGAGAAG 1201 GTCTAGAGAA AGCACAAAGA TTATTGAAAC AGTCGCAGAA ACGAGATTAT TATAAAATCT 1261 TGGGAGTAAA AAGAAATGCC AAAAAGCAAG AAATTATTAA AGCATACCGA AAATTAGCAC 1321 TGCAGTGGCA CCCAGATAAC TTCCAGAATG AAGAAGAAAA GAAAAAAGCT GAGAAAAAGT 1381 TCATTGATAT AGCAGCTGCT AAAGAAGTCC TCTCTGATCC AGAAATGAGA AAGAAGTTTG 1441 ACGACGGAGA AGATCCTTTG GATGCAGAGA GCCAGCAAGG AGGCGGCGGC AACCCTTTCC 1501 ACAGAAGCTG GAACTCATGG CAAGGGTTCA ATCCCTTCAG CTCAGGCGGA CCATTTAGAT 1561 TTAAATTCCA CTTCAATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGCAGCA GATACCCGAA 1741 GAAGGCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt