Construct: ORF TRCN0000466474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006244.1_s317c1
- Derived from:
- ccsbBroadEn_05062
- DNA Barcode:
- GGTTATCGTATTAGTCCACTGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC66A1L (152078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152078 | SLC66A1L | solute carrier family 66 me... | NM_001099777.3 | 100% | 100% | |
| 2 | human | 152078 | SLC66A1L | solute carrier family 66 me... | XM_017005754.1 | 88.7% | 82.6% | (many diffs) |
| 3 | human | 152078 | SLC66A1L | solute carrier family 66 me... | NM_001130002.2 | 83.2% | 78.5% | (many diffs) |
| 4 | human | 152078 | SLC66A1L | solute carrier family 66 me... | NM_001130001.2 | 69.8% | 56.4% | (many diffs) |
| 5 | human | 152078 | SLC66A1L | solute carrier family 66 me... | NM_001243729.1 | 61% | 60.1% | 213_214insATTTTTACAGCCATC;216_217ins123 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 447
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAAAGT CGTGGGAAAC TATAGAGTCA 121 ACACTGCAAA CTCAAGCACT GATACGTCCG GAGAGCACTT GACCTGCCTT AGAAGTCAGC 181 TCTTTGTAGC CTACAGAAAT GGAAGAGTGG ATGAAGCAGT CTCTCTGGGT TTTCTGGATT 241 GCTGGATAGG TGGAGACCTG ACAAATTTCA AAGGCTGCTA CCTGACTAAC CAACTGCCTA 301 TTCAGATTTT TACAGCCATC TTCGACATGA ACACGGATGT AATCATACTC TCACAATTCA 361 TGTACTACAG GTTAAAGAAT CAGAAGAAAA AAATTTCTGA AGAAGATCTA CGGAAGGAAC 421 TTCATTTCTG CTGTTTGCAT TGGCCATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGTTATCG 601 TATTAGTCCA CTGGGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt