Construct: ORF TRCN0000466500
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015948.1_s317c1
- Derived from:
- ccsbBroadEn_13642
- DNA Barcode:
- GCTTTGTGTAGGTATACCCTTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUSBP2 (387036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466500
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_133000.1 | 51.6% | (many diffs) | |
2 | human | 100049076 | GUSBP9 | GUSB pseudogene 9 | NR_033968.1 | 43.5% | (many diffs) | |
3 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_132998.1 | 39.3% | (many diffs) | |
4 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_003660.2 | 35.5% | (many diffs) | |
5 | human | 728411 | GUSBP1 | GUSB pseudogene 1 | NR_027028.2 | 35.4% | (many diffs) | |
6 | human | 102725009 | LOC102725009 | putative inactive beta-gluc... | XM_017030249.2 | 33.2% | 11.4% | 1_268del;450_451ins97 |
7 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_024054.2 | 27.3% | 1_189del;434G>A;469_1016del | |
8 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_132997.1 | 26.6% | (many diffs) | |
9 | human | 387036 | GUSBP2 | GUSB pseudogene 2 | NR_003504.3 | 23.1% | 1_585del;757C>G;865_1200del | |
10 | human | 2990 | GUSB | glucuronidase beta | XM_017012093.2 | 22.5% | 16.4% | (many diffs) |
11 | human | 2990 | GUSB | glucuronidase beta | XM_017012092.1 | 20.6% | 15% | (many diffs) |
12 | human | 2990 | GUSB | glucuronidase beta | XM_011516114.2 | 19.8% | 14.4% | (many diffs) |
13 | human | 2990 | GUSB | glucuronidase beta | NM_001293105.2 | 19.5% | 14.2% | (many diffs) |
14 | human | 2990 | GUSB | glucuronidase beta | XM_017012091.1 | 19.5% | 14.2% | (many diffs) |
15 | human | 2990 | GUSB | glucuronidase beta | NM_001293104.2 | 18.3% | 13.3% | (many diffs) |
16 | human | 653188 | GUSBP3 | GUSB pseudogene 3 | NR_027386.2 | 17.6% | (many diffs) | |
17 | human | 2990 | GUSB | glucuronidase beta | XM_011516113.1 | 17.4% | 12.7% | (many diffs) |
18 | human | 2990 | GUSB | glucuronidase beta | NM_001284290.2 | 16.7% | 12.2% | (many diffs) |
19 | human | 11042 | GUSBP15 | GUSB pseudogene 15 | NR_034021.1 | 15.4% | (many diffs) | |
20 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_029426.1 | 14.3% | 1_481del;726G>A;761_1943del | |
21 | human | 2990 | GUSB | glucuronidase beta | XM_005250297.4 | 14.1% | 10.2% | (many diffs) |
22 | human | 2990 | GUSB | glucuronidase beta | NM_000181.4 | 13% | 9.4% | (many diffs) |
23 | human | 2990 | GUSB | glucuronidase beta | XR_001744660.2 | 12.6% | (many diffs) | |
24 | human | 2990 | GUSB | glucuronidase beta | XR_927461.3 | 11.7% | (many diffs) | |
25 | human | 653080 | GUSBP16 | GUSB pseudogene 16 | NR_146391.1 | 11.5% | (many diffs) | |
26 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | XR_002958912.1 | 5.5% | 1_846del;1091G>A;1126_4984del | |
27 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_132999.1 | 5.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 369
- ORF length:
- 279
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTGATTGC TCACACCAAA GCCTTGGACC 121 CCTCCCAGCC TGTGACCTTT GTGACCAACT CCACCTACGC AGCAGACAAG GGGGCTCTGT 181 ATGTGGATGT GATCCGTGTG AACAGCTACT ACTCTTGGTA TCGCAACTAC GGGCACCTGG 241 AGTTGATTCA GCTGCAGCTG GCCGCCCAGT TTGAGAATTG GTGTAAGACA TCACAATCCC 301 ATTATTCAGA GCGCGTATGG AGTGGAAACG CTTATAGGGT TTCACCAGGA TCCACCTCTG 361 ATGTTCAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTTTG TGTAGGTATA CCCTTCGCAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt