Construct: ORF TRCN0000466505
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004783.1_s317c1
- Derived from:
- ccsbBroadEn_07574
- DNA Barcode:
- TTATACGCAAACTTCTCCTGACGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AP3S2 (10239)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466505
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10239 | AP3S2 | adaptor related protein com... | NM_005829.5 | 99.8% | 5T>G | |
| 2 | human | 100526783 | ARPIN-AP3S2 | ARPIN-AP3S2 readthrough | NM_001199058.2 | 46.8% | (many diffs) | |
| 3 | human | 10239 | AP3S2 | adaptor related protein com... | NR_037582.2 | 10.3% | (many diffs) | |
| 4 | human | 10239 | AP3S2 | adaptor related protein com... | NR_023361.2 | 10.2% | (many diffs) | |
| 5 | mouse | 11778 | Ap3s2 | adaptor-related protein com... | NM_009682.3 | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 61
- ORF end:
- 67
- ORF length:
- 6
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 atggcatgag tcaggcgatt ctggttttca acaaccatgg gaagccacgg ctagtccgct 121 tctaccagcg tttcccagaa gaaattcaac agcagattgt tcgagagact ttccatctag 181 tcctcaagcg ggatgacaac atctgtaact tcttGGAGGG TGGAAGTTTG ATTGGTGGCT 241 CTGACTACAA ACTGATCTAC CGGCACTATG CTACCCTCTA CTTTGTATTT TGTGTGGATT 301 CCTCAGAGAG TGAACTTGGA ATCTTGGACC TCATCCAGGT TTTTGTGGAA ACTCTGGATA 361 AGTGTTTCGA AAATGTGTGT GAATTGGATT TGATCTTCCA TATGGATAAG GTGCACTACA 421 TCCTCCAGGA GGTGGTGATG GGTGGGATGG TGTTGGAAAC AAACATGAAT GAAATCGTGG 481 CTCAGATTGA GGCTCAAAAC AGGCTGGAGA AATCCGAGGG TGGCCTTTCA GCAGCCCCTG 541 CGCGGGCTGT GTCTGCTGTG AAAAACATCA ACCTGCCAGA GATTCCTCGG AACATCAACA 601 TTGGCGATCT CAACATCAAA GTTCCCAACC TGTCCCAGTT TGTCTGCCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA TTATACGCAA ACTTCTCCTG ACGAACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt