Construct: ORF TRCN0000466510
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001587.1_s317c1
- Derived from:
- ccsbBroadEn_14128
- DNA Barcode:
- AACAGGGACGATGTAACGGTATTA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC40A1 (30061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466510
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30061 | SLC40A1 | solute carrier family 40 me... | NM_014585.5 | 99.8% | 99.6% | 563C>T;1709delT |
| 2 | human | 30061 | SLC40A1 | solute carrier family 40 me... | XM_005246505.2 | 92.8% | 92.6% | 0_1ins120;443C>T;1589delT |
| 3 | human | 30061 | SLC40A1 | solute carrier family 40 me... | XM_017003938.2 | 92.8% | 92.6% | 0_1ins120;443C>T;1589delT |
| 4 | mouse | 53945 | Slc40a1 | solute carrier family 40 (i... | NM_016917.2 | 86.4% | 89.8% | (many diffs) |
| 5 | mouse | 53945 | Slc40a1 | solute carrier family 40 (i... | XM_006496137.2 | 86.4% | 89.8% | (many diffs) |
| 6 | mouse | 53945 | Slc40a1 | solute carrier family 40 (i... | XM_017321709.1 | 86.4% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1779
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cagggcggga gatcacaacc gccagagagg atgctgtgga tccttggccg 121 actacctgac ctctgcaaaa ttccttctct accttggtca ttctctctct acttggggag 181 atcggatgtg gcactttgcg gtgtctgtgt ttctggtaga gctctatgga aacagcctcc 241 ttttgacagc agtctacggg ctggtggtgg cagggtctgt tctggtcctg ggagccatca 301 tcggtgactg ggtggacaag aatgctagac ttaaagtggc ccagacctcg ctggtggtac 361 agaatgtttc agtcatcctg tgtggaatca tcctgatgat ggttttctta cataaacatg 421 agcttctgac catgtaccat ggatgggttc tcacttcctg ctatatcctg atcatcacta 481 ttgcaaatat tgcaaatttg gccagtactg ctactgcaat cacaatccaa agggattgga 541 ttgttgttgt tgcaggagaa gacagaagca aactagcaaa tatgaatgcc acaatacgaa 601 ggattgacca gttaaccaac atcttagtcc ccatggctgt tggccagatt atgacatttg 661 gctccccagt catcggctgt ggctttattt cgggatggaa cttggtatcc atgtgcgtgg 721 agtacgttct gctctggaag gtttaccaga aaaccccagc tctagctgtg aaagctggtc 781 ttaaagaaga ggaaactgaa ttgaaacagc tgaatttaca caaagatact gagccaaaac 841 ccctggaggg aactcatcta atgggtgtga aagactctaa catccatgag cttgaacatg 901 agcaagagcc tacttgtgcc tcccagatgg ctgagccctt ccgtaccttc cgagatggat 961 gggtctccta ctacaaccag cctgtgtttc tggctggcat gggtcttgct ttcctttata 1021 tgactgtcct gggctttgac tgcatcacca cagggtacgc ctacactcag ggactgagtg 1081 gttccatcct cagtattttg atgggagcat cagctataac tggaataatg ggaactgtag 1141 cttttacttg gctacgtcga aaatgtggtt tggttcggac aggtctgatc tcaggattgg 1201 cacagctttc ctgtttgatc ttgtgtgtga tctctgtatt catgcctgga agccccctgg 1261 acttgtccgt ttctcctttt gaagatatcc gatcaaggtt cattcaagga gagtcaatta 1321 cacctaccaa gatacctgaa attacaactg aaatatacat gtctaatggg tctaattctg 1381 ctaatattgt cccggagaca agtcctgaat ctgtgcccat aatctctgtc agtctgctgt 1441 ttgcaggcgt cattgctgct agaatcggTC TTTGGTCCTT TGATTTAACT GTGACACAGT 1501 TGCTGCAAGA AAATGTAATT GAATCTGAAA GAGGCATTAT AAATGGTGTA CAGAACTCCA 1561 TGAACTATCT TCTTGATCTT CTGCATTTCA TCATGGTCAT CCTGGCTCCA AATCCTGAAG 1621 CTTTTGGCTT GCTCGTATTG ATTTCAGTCT CCTTTGTGGC AATGGGCCAC ATTATGTATT 1681 TCCGATTTGC CCAAAATACT CTGGGAAACA AGCTCTTTGC TTGCGGTCCT GATGCAAAAG 1741 AAGTTAGGAA GGAAAATCAA GCAAATACAT CTGTGTTTGC CCAACTTTCT TGTACAAAGT 1801 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1861 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1921 CGAAACAGGG ACGATGTAAC GGTATTAACG CGTTAAGTCg acaatcaacc tctggattac 1981 aaaatttgtg aaagatt