Transcript: Human XM_017003938.2

PREDICTED: Homo sapiens solute carrier family 40 member 1 (SLC40A1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SLC40A1 (30061)
Length:
3348
CDS:
721..2316

Additional Resources:

NCBI RefSeq record:
XM_017003938.2
NBCI Gene record:
SLC40A1 (30061)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003938.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000427851 TTGTTCAAGACTAGCTAATTT pLKO_005 2701 3UTR 100% 15.000 21.000 N SLC40A1 n/a
2 TRCN0000415007 ACGAAGGATTGACCAGTTAAC pLKO_005 1131 CDS 100% 10.800 15.120 N SLC40A1 n/a
3 TRCN0000043313 CGCTGGTGGTACAGAATGTTT pLKO.1 884 CDS 100% 5.625 4.500 N SLC40A1 n/a
4 TRCN0000043314 GCCACATTATGTATTTCCGAT pLKO.1 2201 CDS 100% 2.640 2.112 N SLC40A1 n/a
5 TRCN0000043315 GCATCAGCTATAACTGGAATA pLKO.1 1642 CDS 100% 10.800 7.560 N SLC40A1 n/a
6 TRCN0000079802 CCTGATCATCACTATTGCAAA pLKO.1 1002 CDS 100% 4.950 3.465 N Slc40a1 n/a
7 TRCN0000043316 GCAATCACAATCCAAAGGGAT pLKO.1 1051 CDS 100% 2.640 1.848 N SLC40A1 n/a
8 TRCN0000043317 CCTGTGTGGAATCATCCTGAT pLKO.1 912 CDS 100% 4.050 2.430 N SLC40A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003938.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14128 pDONR223 100% 92.8% 92.6% None 0_1ins120;443C>T;1589delT n/a
2 TRCN0000466510 AACAGGGACGATGTAACGGTATTA pLX_317 18.4% 92.8% 92.6% V5 (not translated due to frame shift) 0_1ins120;443C>T;1589delT n/a
3 ccsbBroadEn_08161 pDONR223 100% 92.8% 92.8% None 0_1ins120;543T>C;851T>C n/a
4 ccsbBroad304_08161 pLX_304 0% 92.8% 92.8% V5 0_1ins120;543T>C;851T>C n/a
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