Construct: ORF TRCN0000466523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012481.1_s317c1
- Derived from:
- ccsbBroadEn_11291
- DNA Barcode:
- ATTTCTTGGATTTTTATCGGCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUMB (8650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005745.1 | 22.8% | 22.8% | 1_1371del |
| 2 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005744.1 | 22.3% | 22.3% | 1_1404del |
| 3 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001320114.1 | 22.3% | 22.3% | 1_1404del |
| 4 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_003744.5 | 21% | 21% | 1_1515del |
| 5 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005743.1 | 20.7% | 20.7% | 1_1548del |
| 6 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272056.1 | 20.1% | 20.4% | (many diffs) |
| 7 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_010949.2 | 19.8% | 20% | (many diffs) |
| 8 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272055.1 | 18.6% | 18.8% | (many diffs) |
| 9 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515578.1 | 18.6% | 18.8% | (many diffs) |
| 10 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001136075.2 | 18.3% | 18.5% | (many diffs) |
| 11 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515576.1 | 18.3% | 18.5% | (many diffs) |
| 12 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515577.2 | 18.3% | 18.5% | (many diffs) |
| 13 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_011244012.2 | 18.3% | 18.5% | (many diffs) |
| 14 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515575.2 | 17.5% | 17.7% | (many diffs) |
| 15 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515574.2 | 17.3% | 17.5% | (many diffs) |
| 16 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515573.2 | 16.4% | 16.5% | (many diffs) |
| 17 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515572.2 | 16.1% | 16.3% | (many diffs) |
| 18 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073563.1 | 10.1% | (many diffs) | |
| 19 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073564.1 | 9.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctatccagcc ccTAATGTGC CTGTGGTGGG CATCACTCCC TCCCAGATGG 121 TGGCCAACGT ATTTGGCACT GCAGGCCACC CTCAGGCTGC CCATCCCCAT CAGTCACCCA 181 GCCTGGTCAG GCAGCAGACA TTCCCTCACT ACGAGGCAAG CAGTGCTACC ACCAGTCCCT 241 TCTTTAAGCC TCCTGCTCAG CACCTCAACG GTTCTGCAGC TTTCAATGGT GTAGATGATG 301 GCAGGTTGGC CTCAGCAGAC AGGCATACAG AGGTTCCTAC AGGCACCTGC CCAGTGGATC 361 CTTTTGAAGC CCAGTGGGCT GCATTAGAAA ATAAGTCCAA GCAGCGTACT AATCCCTCCC 421 CTACCAACCC TTTCTCCAGT GACTTACAGA AGACGTTTGA AATTGAACTT TACCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAATTT CTTGGATTTT TATCGGCCAC ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt