Construct: ORF TRCN0000466580

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004080.1_s317c1
Derived from:
ccsbBroadEn_09163
DNA Barcode:
GGTACAGTAATTATGTAACTCTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSD2 (84249)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466580
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84249 PSD2 pleckstrin and Sec7 domain ... NM_032289.4 99.9% 100% 633A>G
2 human 84249 PSD2 pleckstrin and Sec7 domain ... XM_017009976.1 99.9% 100% 633A>G
3 human 84249 PSD2 pleckstrin and Sec7 domain ... XM_017009977.1 99.9% 100% 633A>G
4 human 84249 PSD2 pleckstrin and Sec7 domain ... XM_011537696.2 97.4% 97.4% 1_60del;693A>G
5 mouse 74002 Psd2 pleckstrin and Sec7 domain ... NM_028707.4 85.2% 87.8% (many diffs)
6 mouse 74002 Psd2 pleckstrin and Sec7 domain ... XM_017317995.1 85.1% 87.7% (many diffs)
7 mouse 74002 Psd2 pleckstrin and Sec7 domain ... NM_001289602.1 84.8% 87.3% (many diffs)
8 mouse 74002 Psd2 pleckstrin and Sec7 domain ... NR_110349.1 41.2% (many diffs)
9 mouse 74002 Psd2 pleckstrin and Sec7 domain ... XR_001782394.1 33.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2379
ORF length:
2313
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggaggacaag ctcttatctg cagtgcctga ggaaggcgat gccacccgtg
121accccggtcc agagcctgaa gaggagccag gggtccggaa tgggatggcc agtgagggcc
181tgaacagcag cctctgcagc ccagggcacg agcgaagggg caccccagcg gacactgagg
241aacccacgaa ggacccagat gtggccttcc atggcctcag ccttggcctc tctctcacca
301atggcctagc cctggggcca gacttgaaca ttctggaaga ttcagcggag tccaggccct
361ggagggctgg cgtgctggca gagggggaca atgcttccag gagcctctac ccagatgctg
421aggaccctca gctggggttg gatggtcccg gggagccaga tgtgcgggat ggcttcagcg
481ccacgtttga gaagattctg gagtcagagc tgctgcgggg cacccagtac agcagcctcg
541actccctaga cgggctgagc ctcacggatg agagcgacag ctgcgtcagc ttcgaggccc
601ccctcacacc cctcatccag cagcgggccc gtgacagccc tgagccaggg gctgggttgg
661gcattgggga catggcgttt gagggggaca tgggggcggc tggtggtgat ggggagctgg
721gcagccccct gcggcgctcc atctccagca gccgctctga gaatgtcctg agccgcctgt
781ctctcatggc catgcccaat ggattccatg aagatggccc tcagggccca gggggggatg
841aggatgatga tgaggaggac acggacaagt tgctgaactc agccagtgac cccagcctga
901aggatggcct gtcagactca gactctgagc tcagcagctc ggaggggttg gagcctggta
961gtgcagaccc tctggccaac gggtgccagg gggtcagtga agctgctcat cggctggcac
1021gccgtctcta ccacctcgag ggcttccagc gctgtgatgt ggcccggcag ctgggcaaga
1081acaacgagtt tagcaggctg gtggccgggg agtacctcag tttcttcgac ttctcgggct
1141tgactctgga cggagcactc agaacattct tgaaggcctt cccgctgatg ggggagacac
1201aagagcgtga gcgggtcctc acacacttct cccgccggta ctgccagtgc aaccctgatg
1261acagcacttc ggaagatggg atccacacgc tcacctgtgc cctgatgctg ctcaacacgg
1321acctgcacgg ccacaacatt ggcaaaaaga tgtcctgtca gcaattcatt gccaacttgg
1381accagctgaa tgatggccaa gactttgcca aagacctgct gaagaccctt tacaactcca
1441tcaagaatga aaagctggaa tgggccattg atgaggatga gctgaggaaa tccctgtctg
1501agctggtgga tgacaagttc gggacaggca cgaagaaggt gacgcgaatc ctggatggtg
1561gcaacccctt cctggatgtc ccacaggcgc tcagtgccac cacctacaag cacggcgtcc
1621tgacccggaa gactcacgct gacatggatg gcaagaggac gccccgtggg aggcgtggct
1681ggaagaaatt ctacgcagtg ctcaaaggga ccatcctgta cctgcagaag gatgagtaca
1741ggcctgacaa agctctatcg gagggtgacc tgaagaacgc cattcgcgtg catcacgctc
1801tggccaccag ggcctctgac tacagcaaga agtccaacgt gctgaagctt aagacagccg
1861actggagggt attcctcttc caggcaccga gcaaggaaga aatgctgtcc tggatcctca
1921ggatcaacct ggtggcagcc atcttctctg ccccggcctt cccagccgct gtcagctcca
1981tgaagaagtt ctgtcggccc ctgctgccct cctgcaccac ccgcctctgc caggaggagc
2041aactgcggtc TCATGAGAAT AAGTTGAGGC AGCTGACTGC GGAGCTGGCC GAACACAGGT
2101GTCACCCAGT CGAGAGGGGC ATCAAGTCCA AGGAGGCCGA GGAGTACCGG TTGAAGGAGC
2161ACTATCTCAC CTTCGAGAAA AGCCGTTATG AGACCTATAT CCACCTCCTG GCTATGAAAA
2221TCAAAGTGGG CTCAGATGAT CTGGAGCGGA TTGAGGCCCG GCTGGCCACT CTGGAAGGGG
2281ATGACCCTTC TCTCCGGAAG ACACATTCAA GCCCTGCCCT CAGCCAGGGC CATGTGACTG
2341GCAGCAAAAC CACAAAGGAT GCCACTGGGC CTGATACTTA CCCAACTTTC TTGTACAAAG
2401TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2461GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2521ACGAGGTACA GTAATTATGT AACTCTCCAC GCGTTAAGTC gacaatcaac ctctggatta
2581caaaatttgt gaaagatt

Download FASTA (ORF) (Full)