Construct: ORF TRCN0000466580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004080.1_s317c1
- Derived from:
- ccsbBroadEn_09163
- DNA Barcode:
- GGTACAGTAATTATGTAACTCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSD2 (84249)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84249 | PSD2 | pleckstrin and Sec7 domain ... | NM_032289.4 | 99.9% | 100% | 633A>G |
| 2 | human | 84249 | PSD2 | pleckstrin and Sec7 domain ... | XM_017009976.1 | 99.9% | 100% | 633A>G |
| 3 | human | 84249 | PSD2 | pleckstrin and Sec7 domain ... | XM_017009977.1 | 99.9% | 100% | 633A>G |
| 4 | human | 84249 | PSD2 | pleckstrin and Sec7 domain ... | XM_011537696.2 | 97.4% | 97.4% | 1_60del;693A>G |
| 5 | mouse | 74002 | Psd2 | pleckstrin and Sec7 domain ... | NM_028707.4 | 85.2% | 87.8% | (many diffs) |
| 6 | mouse | 74002 | Psd2 | pleckstrin and Sec7 domain ... | XM_017317995.1 | 85.1% | 87.7% | (many diffs) |
| 7 | mouse | 74002 | Psd2 | pleckstrin and Sec7 domain ... | NM_001289602.1 | 84.8% | 87.3% | (many diffs) |
| 8 | mouse | 74002 | Psd2 | pleckstrin and Sec7 domain ... | NR_110349.1 | 41.2% | (many diffs) | |
| 9 | mouse | 74002 | Psd2 | pleckstrin and Sec7 domain ... | XR_001782394.1 | 33.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2379
- ORF length:
- 2313
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggacaag ctcttatctg cagtgcctga ggaaggcgat gccacccgtg 121 accccggtcc agagcctgaa gaggagccag gggtccggaa tgggatggcc agtgagggcc 181 tgaacagcag cctctgcagc ccagggcacg agcgaagggg caccccagcg gacactgagg 241 aacccacgaa ggacccagat gtggccttcc atggcctcag ccttggcctc tctctcacca 301 atggcctagc cctggggcca gacttgaaca ttctggaaga ttcagcggag tccaggccct 361 ggagggctgg cgtgctggca gagggggaca atgcttccag gagcctctac ccagatgctg 421 aggaccctca gctggggttg gatggtcccg gggagccaga tgtgcgggat ggcttcagcg 481 ccacgtttga gaagattctg gagtcagagc tgctgcgggg cacccagtac agcagcctcg 541 actccctaga cgggctgagc ctcacggatg agagcgacag ctgcgtcagc ttcgaggccc 601 ccctcacacc cctcatccag cagcgggccc gtgacagccc tgagccaggg gctgggttgg 661 gcattgggga catggcgttt gagggggaca tgggggcggc tggtggtgat ggggagctgg 721 gcagccccct gcggcgctcc atctccagca gccgctctga gaatgtcctg agccgcctgt 781 ctctcatggc catgcccaat ggattccatg aagatggccc tcagggccca gggggggatg 841 aggatgatga tgaggaggac acggacaagt tgctgaactc agccagtgac cccagcctga 901 aggatggcct gtcagactca gactctgagc tcagcagctc ggaggggttg gagcctggta 961 gtgcagaccc tctggccaac gggtgccagg gggtcagtga agctgctcat cggctggcac 1021 gccgtctcta ccacctcgag ggcttccagc gctgtgatgt ggcccggcag ctgggcaaga 1081 acaacgagtt tagcaggctg gtggccgggg agtacctcag tttcttcgac ttctcgggct 1141 tgactctgga cggagcactc agaacattct tgaaggcctt cccgctgatg ggggagacac 1201 aagagcgtga gcgggtcctc acacacttct cccgccggta ctgccagtgc aaccctgatg 1261 acagcacttc ggaagatggg atccacacgc tcacctgtgc cctgatgctg ctcaacacgg 1321 acctgcacgg ccacaacatt ggcaaaaaga tgtcctgtca gcaattcatt gccaacttgg 1381 accagctgaa tgatggccaa gactttgcca aagacctgct gaagaccctt tacaactcca 1441 tcaagaatga aaagctggaa tgggccattg atgaggatga gctgaggaaa tccctgtctg 1501 agctggtgga tgacaagttc gggacaggca cgaagaaggt gacgcgaatc ctggatggtg 1561 gcaacccctt cctggatgtc ccacaggcgc tcagtgccac cacctacaag cacggcgtcc 1621 tgacccggaa gactcacgct gacatggatg gcaagaggac gccccgtggg aggcgtggct 1681 ggaagaaatt ctacgcagtg ctcaaaggga ccatcctgta cctgcagaag gatgagtaca 1741 ggcctgacaa agctctatcg gagggtgacc tgaagaacgc cattcgcgtg catcacgctc 1801 tggccaccag ggcctctgac tacagcaaga agtccaacgt gctgaagctt aagacagccg 1861 actggagggt attcctcttc caggcaccga gcaaggaaga aatgctgtcc tggatcctca 1921 ggatcaacct ggtggcagcc atcttctctg ccccggcctt cccagccgct gtcagctcca 1981 tgaagaagtt ctgtcggccc ctgctgccct cctgcaccac ccgcctctgc caggaggagc 2041 aactgcggtc TCATGAGAAT AAGTTGAGGC AGCTGACTGC GGAGCTGGCC GAACACAGGT 2101 GTCACCCAGT CGAGAGGGGC ATCAAGTCCA AGGAGGCCGA GGAGTACCGG TTGAAGGAGC 2161 ACTATCTCAC CTTCGAGAAA AGCCGTTATG AGACCTATAT CCACCTCCTG GCTATGAAAA 2221 TCAAAGTGGG CTCAGATGAT CTGGAGCGGA TTGAGGCCCG GCTGGCCACT CTGGAAGGGG 2281 ATGACCCTTC TCTCCGGAAG ACACATTCAA GCCCTGCCCT CAGCCAGGGC CATGTGACTG 2341 GCAGCAAAAC CACAAAGGAT GCCACTGGGC CTGATACTTA CCCAACTTTC TTGTACAAAG 2401 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2461 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2521 ACGAGGTACA GTAATTATGT AACTCTCCAC GCGTTAAGTC gacaatcaac ctctggatta 2581 caaaatttgt gaaagatt