Construct: ORF TRCN0000466593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000657.1_s317c1
- Derived from:
- ccsbBroadEn_00661
- DNA Barcode:
- CAATGATTTCAGCATGCTAGCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNGT2 (2793)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2793 | GNGT2 | G protein subunit gamma tra... | NM_001198754.2 | 100% | 100% | |
| 2 | human | 2793 | GNGT2 | G protein subunit gamma tra... | NM_001198755.1 | 100% | 100% | |
| 3 | human | 2793 | GNGT2 | G protein subunit gamma tra... | NM_001198756.1 | 100% | 100% | |
| 4 | human | 2793 | GNGT2 | G protein subunit gamma tra... | NM_031498.2 | 100% | 100% | |
| 5 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | NM_001038664.2 | 85% | 82.6% | (many diffs) |
| 6 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | NM_001284393.1 | 85% | 82.6% | (many diffs) |
| 7 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | NM_001284397.1 | 85% | 82.6% | (many diffs) |
| 8 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | NM_023121.2 | 85% | 82.6% | (many diffs) |
| 9 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | XM_006532231.3 | 85% | 82.6% | (many diffs) |
| 10 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | XM_011248737.1 | 85% | 82.6% | (many diffs) |
| 11 | mouse | 14710 | Gngt2 | guanine nucleotide binding ... | XM_017314274.1 | 85% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 297
- ORF length:
- 207
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCCAGGA TCTCAGCGAG AAGGACCTGT 121 TGAAGATGGA GGTGGAGCAG CTGAAGAAAG AAGTGAAAAA CACAAGAATT CCGATTTCCA 181 AAGCGGGAAA GGAAATCAAG GAGTACGTGG AGGCCCAAGC AGGAAACGAT CCTTTTCTCA 241 AAGGCATCCC TGAGGACAAG AATCCCTTCA AGGAGAAAGG TGGCTGTCTG ATAAGCTGCC 301 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 361 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 421 ATATATCTTG TGGAAAGGAC GACAATGATT TCAGCATGCT AGCCTAACGC GTTAAGTCga 481 caatcaacct ctggattaca aaatttgtga aagatt