Construct: ORF TRCN0000466597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017525.1_s317c1
- Derived from:
- ccsbBroadEn_08770
- DNA Barcode:
- GGCAAACCACCGGTAATATGTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAP2C (57826)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466597
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | NM_001271186.2 | 99.8% | 99.4% | 404C>T |
| 2 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | NM_021183.5 | 99.8% | 99.4% | 404C>T |
| 3 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | XM_011531376.1 | 99.8% | 99.4% | 404C>T |
| 4 | human | 5911 | RAP2A | RAP2A, member of RAS oncoge... | NM_021033.7 | 75.1% | 90.7% | (many diffs) |
| 5 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | NM_001271187.1 | 63.7% | 63.3% | 0_1ins198;206C>T |
| 6 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | XM_006724775.2 | 63.7% | 63.3% | 0_1ins198;206C>T |
| 7 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | XR_430548.1 | 16.3% | 1_154del;558C>T;704_3356del | |
| 8 | human | 57826 | RAP2C | RAP2C, member of RAS oncoge... | NR_073150.2 | 15% | (many diffs) | |
| 9 | mouse | 72065 | Rap2c | RAP2C, member of RAS oncoge... | NM_172413.2 | 94.7% | 99.4% | (many diffs) |
| 10 | mouse | 72065 | Rap2c | RAP2C, member of RAS oncoge... | XM_006541545.2 | 94.7% | 99.4% | (many diffs) |
| 11 | mouse | 72065 | Rap2c | RAP2C, member of RAS oncoge... | XM_006541546.2 | 94.7% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggaatacaag gtagtggtgt tagggagtgg aggggttggc aaatctgccc 121 ttactgtgca gtttgtcact gggactttca ttgagaaata tgaccccacc attgaagatt 181 tctaccgcaa agagatcgaa gtggactctt ccccctccgt gctggaaatt ctggacaccg 241 caggaactga gcagtttgcc tccatgagag atctctacat caaaaacggc caaggtttca 301 tcctggttta tagcctggtt aatcaacagt cttTTCAGGA TATCAAGCCA ATGAGAGATC 361 AAATTGTCAG AGTGAAGAGA TATGAAAAAG TCCCACTAAT CCTAGTAGGA AATAAAGTGG 421 ATCTGGAACC AGAAAGAGAG GTTATGTCTT CAGAAGGCAG AGCTCTGGTT CAAGAATGGG 481 GCTGTCCTTT CATGGAGACA TCGGCAAAAA GTAAATCAAT GGTGGATGAA CTTTTTGCTG 541 AGATCGTCAG GCAAATGAAC TATTCATCCC TGCCGGAGAA GCAAGATCAG TGTTGTACAA 601 CTTGTGTCGT CCAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGCAAACCAC CGGTAATATG 781 TCGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt