Construct: ORF TRCN0000466629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006022.1_s317c1
- Derived from:
- ccsbBroadEn_02919
- DNA Barcode:
- CTCTCGTGCCAGATATTTGGACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIFBP (26128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26128 | KIFBP | kinesin family binding protein | NM_015634.4 | 100% | 100% | |
| 2 | human | 26128 | KIFBP | kinesin family binding protein | XM_017016067.1 | 57.1% | 57.1% | 0_1ins798 |
| 3 | mouse | 72320 | Kif1bp | KIF1 binding protein | NM_028197.2 | 84.9% | 91.9% | (many diffs) |
| 4 | mouse | 72320 | Kif1bp | KIF1 binding protein | XM_006514192.3 | 44.3% | 47% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1929
- ORF length:
- 1863
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaacgttccg tgggcagagg tctgcgagaa attccaggcg gcgctcgctc 121 tgtcgcgggt ggaactgcat aaaaatccgg agaaggaacc atacaagtcc aaatacagcg 181 cccgggcgct actggaagag gtcaaggcgc tgctcggccc tgcgcctgag gacgaggatg 241 agcggcctga ggccgaggac ggcccgggtg ccggtgacca cgccctgggg ctgccggctg 301 aggtggtgga gcccgagggg cccgtcgccc agcgagcggt gaggctggca gtcatcgagt 361 tccacctcgg ggtgaaccac atcgacacgg aggagctgtc ggcgggggag gagcacctgg 421 tgaaatgcct gcggctgctg cgcaggtacc ggctctcgca cgactgcatc tctctctgca 481 tccaggcgca gaataacctg ggtatcttgt ggtctgaaag agaagaaatt gaaactgcac 541 aggcttacct agagtcatca gaagcactat ataatcagta tatgaaagag gttgggagtc 601 ctcctcttga tcctactgag cgttttcttc ctgaagaaga gaaacttact gaacaagaga 661 gatcaaaaag atttgaaaag gtttatactc ataacctata ttacctagct caagtctacc 721 agcatctgga aatgtttgag aaggctgctc actattgcca tagtacacta aaacgccagc 781 ttgagcacaa tgcctaccat cctatagagt gggctatcaa tgctgctacc ttgtcacagt 841 tttacatcaa taagctatgc tttatggagg ccaggcactg tttatcagct gctaatgtca 901 tttttggtca aactggaaag atctcagcca cagaagacac tcctgaagct gaaggagaag 961 tgccagagct ttatcatcaa agaaaggggg aaatagcaag gtgctggatc aaatactgtt 1021 tgactctcat gcagaatgcc caactctcca tgcaggacaa cataggagag cttgatcttg 1081 ataaacagtc tgaacttaga gctttaagga aaaaagaact agatgaggag gaaagcattc 1141 ggaaaaaagc tgtgcagttt ggaaccggtg aactgtgtga tgccatctct gcagtagaag 1201 agaaagtgag ctacttgaga cctttagatt ttgaagaagc cagagaactt ttcttattgg 1261 gtcagcacta tgtctttgag gcaaaagagt tctttcagat tgatggttat gtcactgacc 1321 atattgaagt tgtccaagac cacagtgctc tgtttaaggt gcttgcattc tttgaaactg 1381 acatggagag acggtgcaag atgcataaac gcagaatagc catgctagag cccctaactg 1441 tagacctgaa tccacagtat tatctgttgg tcaacagaca gatccagttt gaaattgcac 1501 atgcttacta tgatatgatg gatttgaagg ttgccattgc tgacaggcta agggatcctg 1561 attcacacat tGTAAAAAAA ATAAATAATC TTAATAAGTC AGCACTGAAG TACTACCAGC 1621 TCTTCTTAGA CTCCCTGAGA GACCCAAATA AAGTATTCCC TGAGCATATA GGGGAAGATG 1681 TTCTTCGCCC TGCCATGTTA GCTAAGTTTC GAGTTGCCCG TCTCTATGGC AAAATCATTA 1741 CTGCAGATCC CAAGAAAGAG CTGGAAAATT TGGCAACATC ATTGGAACAT TACAAATTTA 1801 TTGTTGATTA CTGTGAAAAG CATCCTGAGG CCGCCCAGGA AATAGAAGTT GAGCTAGAAC 1861 TTAGTAAAGA GATGGTTAGT CTTCTCCCAA CAAAAATGGA GAGATTCAGA ACCAAGATGG 1921 CCCTGACTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1981 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2041 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCTCG TGCCAGATAT TTGGACCAAC 2101 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt