Construct: ORF TRCN0000466651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002520.1_s317c1
- Derived from:
- ccsbBroadEn_11139
- DNA Barcode:
- GTAGTATTTCTTAAATAGCTGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A1 (6557)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6557 | SLC12A1 | solute carrier family 12 me... | NM_000338.3 | 38.3% | 37% | (many diffs) |
| 2 | human | 6557 | SLC12A1 | solute carrier family 12 me... | XM_005254606.2 | 37.3% | 36.3% | (many diffs) |
| 3 | human | 6557 | SLC12A1 | solute carrier family 12 me... | NM_001184832.2 | 37.1% | 36.1% | (many diffs) |
| 4 | mouse | 20495 | Slc12a1 | solute carrier family 12, m... | NM_183354.2 | 33.8% | 34.1% | (many diffs) |
| 5 | mouse | 20495 | Slc12a1 | solute carrier family 12, m... | NM_001079690.1 | 32.7% | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc actgaacaac tcttccaatg tatttctgga ttcagtgccc agtaatacca 121 atcgctttca agttagtgtc ataaatgaga accatgagag cagtgcagct gcagatgaca 181 atactgaccc accacattat gaagaaacct cttttgggga tgaagctcag aaaagactca 241 gaatcagctt taggcctggg aatcaggagt gctatgacaa tttcctccaa agtggagaaa 301 ctgctaaaac agatgccagt tttcacgctt atgattctca cacaaacaca tactatctac 361 aaacttttgg ccacaacacc atggatgccg ttcccaagat agagtactat cgtaacaccg 421 gcagcatcag tgggcccaag gtcaaccgac ccagcctgct tgagattcac gagcaactcg 481 caaagaatgt ggcagtcacc ccaagttcag ctgacagagt tgctaacggt gatgggatac 541 ctggagatga acaagctgaa aataaggaag atgatcaagc tggtgttgtg aagtttggat 601 gggtgaaagg tgtgctggta agatgcatgc tgaacatctg gggagtcatg ctcttcattc 661 gcctctcctg gattgttgga gaagctggaa ttggtcttgg agttctcata attcttcttt 721 ccaccatggt aacttctatt actgggttgt caacttctgc gatagcaact aacgggtttg 781 ttcgtggagg tggggcctac tatcttattt ccagaagttt agggcccgag ttcggtgggt 841 caataggcct gatctttgct tttgctaatg cagtggctgt tgctatgtat gtggtgggat 901 ttgctgagac tgtagtagaT CTTCTTAAGG AGAGTGATTC GATGATGGTG GATCCAACCA 961 ATGACATCCG GATTATAGGC TCCATCACAG TGGTGATTCT TCTAGGAATT TCAGTAGCTG 1021 GAATGGAATG GGAGGCAAAG GCCCAAGTCA TTCTTCTGGT CATTCTTCTA ATTGCTATTG 1081 CAAACTTCTT CATTGGAACT GTCATTCCAT CCAACAATGA GAAAAAGTCC AGAGGTTTCT 1141 TTAATTACCA AGCATCAATA TTTGCAGAAA ACTTTGGGCC ACGCTTCACA AAGGGTGAAG 1201 GCTTCTTCTC TGTCTTTGCC ATTTTTTTCC CAGCAGCTAC TGGGATTCTT GCTGGTGCCA 1261 ATATCTCAGG AGATTTGGAG GCACTGAGGA AACAAGGAGC TTCACCTCTC CCTCAAGGAG 1321 CTCAGAGTCG AAGGAGGAGA CAGACTTCCC TTATATGCCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 AGTAGTATTT CTTAAATAGC TGGAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt