Construct: ORF TRCN0000466652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005642.1_s317c1
- Derived from:
- ccsbBroadEn_03833
- DNA Barcode:
- AGTTAGTTTTTCAGTAACCATGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDFY1 (57590)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466652
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57590 | WDFY1 | WD repeat and FYVE domain c... | NM_020830.5 | 100% | 100% | |
| 2 | mouse | 69368 | Wdfy1 | WD repeat and FYVE domain c... | NM_001111279.1 | 88.8% | 97.8% | (many diffs) |
| 3 | mouse | 69368 | Wdfy1 | WD repeat and FYVE domain c... | XM_006496543.2 | 65.4% | 72.6% | (many diffs) |
| 4 | mouse | 69368 | Wdfy1 | WD repeat and FYVE domain c... | XM_017322386.1 | 48.3% | 48.5% | (many diffs) |
| 5 | mouse | 69368 | Wdfy1 | WD repeat and FYVE domain c... | XM_017322387.1 | 48.3% | 48.5% | (many diffs) |
| 6 | mouse | 69368 | Wdfy1 | WD repeat and FYVE domain c... | NM_027057.3 | 39% | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1296
- ORF length:
- 1230
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccgaaatc cactccaggc cgcagagcag ccgcccggtg ctgctgagca 121 agatcgaggg gcaccaggac gccgtcacgg ccgcgctgct catccccaag gaggacggcg 181 tgatcacggc cagcgaggac agaaccatcc gggtatggct gaaaagagac agtggtcaat 241 actggcccag catttaccac acaatggcct ctccttgctc tgctatggct taccatcatg 301 acagcagacg gatatttgtg ggccaggata atggagctgt aatggaattt cacgtttctg 361 aagattttaa taaaatgaac tttatcaaga cctacccagc tcatcagaac cgggtgtctg 421 cgattatctt cagcttggcc acagagtggg tgatcagtac cggccacgac aagtgtgtga 481 gctggatgtg cacgcggagc gggaacatgc tcgggaggca cttcttcacg tcctgggctt 541 cgtgtctgca atatgacttt gacactcagt atgctttcgt tggtgattat tctgggcaga 601 tcaccctgct gaagcttgaa cagaacacgt gttcagtcat cacaaccctc aaaggacatg 661 aaggtagtgt cgcctgcctc tggtgggacc ctattcagcg gttactcttc tcaggagcat 721 ctgacaacag catcatcatg tgggacatcg gaggaaggaa aggccggacg ctgttacttc 781 agggccatca tgacaaggtg cagtcgctgt gctaccttca gctcaccagg cagctcgtct 841 cctgttcctc ggacggcgga attgcagtgt ggaacatgga tgttagcaga gaagaggctc 901 cTCAGTGGTT GGAAAGTGAT TCTTGTCAGA AATGTGAGCA GCCATTTTTC TGGAACATAA 961 AGCAGATGTG GGACACCAAG ACGCTGGGGC TAAGACAACA TCACTGCAGG AAATGCGGGC 1021 AGGCTGTCTG CGGGAAGTGC AGCAGCAAGC GCTCAAGTTA CCCAGTCATG GGCTTCGAGT 1081 TCCAAGTCCG GGTTTGTGAT TCTTGTTACG ACTCCATCAA AGATGAAGAT CGGACTTCTC 1141 TAGCGACCTT TCATGAAGGA AAACATAACA TTTCCCACAT GTCCATGGAC ATTGCCAGGG 1201 GACTGATGGT GACCTGTGGG ACCGACCGCA TTGTAAAGAT CTGGGACATG ACACCTGTGG 1261 TGGGCTGCAG TCTGGCGACT GGGTTTTCTC CGCACTGCCC AACTTTCTTG TACAAAGTGG 1321 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1381 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1441 AAGTTAGTTT TTCAGTAACC ATGAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1501 aatttgtgaa agatt