Transcript: Mouse NM_001111279.1

Mus musculus WD repeat and FYVE domain containing 1 (Wdfy1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Wdfy1 (69368)
Length:
4566
CDS:
70..1302

Additional Resources:

NCBI RefSeq record:
NM_001111279.1
NBCI Gene record:
Wdfy1 (69368)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001111279.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000103689 CGGATATTTGTGGGCCAGGAT pLKO.1 313 CDS 100% 2.640 2.112 N Wdfy1 n/a
2 TRCN0000324426 CGGATATTTGTGGGCCAGGAT pLKO_005 313 CDS 100% 2.640 2.112 N Wdfy1 n/a
3 TRCN0000103685 GCTGTGATGGAATTTCACGTT pLKO.1 340 CDS 100% 2.640 2.112 N Wdfy1 n/a
4 TRCN0000324358 GCTGTGATGGAATTTCACGTT pLKO_005 340 CDS 100% 2.640 2.112 N Wdfy1 n/a
5 TRCN0000136568 CAGTGTGGAACATGGATGTTA pLKO.1 869 CDS 100% 5.625 3.938 N WDFY1 n/a
6 TRCN0000103686 ACCATCCGAGTATGGCTGAAA pLKO.1 208 CDS 100% 4.950 3.465 N Wdfy1 n/a
7 TRCN0000324428 ACCATCCGAGTATGGCTGAAA pLKO_005 208 CDS 100% 4.950 3.465 N Wdfy1 n/a
8 TRCN0000103687 CCGCGTGTCTGCTATCATCTT pLKO.1 414 CDS 100% 4.950 3.465 N Wdfy1 n/a
9 TRCN0000324356 CCGCGTGTCTGCTATCATCTT pLKO_005 414 CDS 100% 4.950 3.465 N Wdfy1 n/a
10 TRCN0000103688 CCACGACAGCAGGCGGATATT pLKO.1 300 CDS 100% 4.400 3.080 N Wdfy1 n/a
11 TRCN0000353942 CCACGACAGCAGGCGGATATT pLKO_005 300 CDS 100% 4.400 3.080 N Wdfy1 n/a
12 TRCN0000054408 ACGCCTTTAATCCCAGCACTT pLKO.1 2661 3UTR 100% 4.050 2.025 Y Mtif2 n/a
13 TRCN0000088533 CGGGAGGCAGAGGCAGGCAAT pLKO.1 2681 3UTR 100% 0.000 0.000 Y Trrap n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001111279.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03833 pDONR223 100% 88.8% 97.8% None (many diffs) n/a
2 ccsbBroad304_03833 pLX_304 0% 88.8% 97.8% V5 (many diffs) n/a
3 TRCN0000466652 AGTTAGTTTTTCAGTAACCATGAG pLX_317 32.4% 88.8% 97.8% V5 (many diffs) n/a
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