Construct: ORF TRCN0000466660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013728.1_s317c1
- Derived from:
- ccsbBroadEn_08678
- DNA Barcode:
- AGCCGATCACTGAGGCTACCCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGMA (56963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466660
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56963 | RGMA | repulsive guidance molecule... | NM_001166286.2 | 99.8% | 99.7% | 1269C>G;1301G>C |
| 2 | human | 56963 | RGMA | repulsive guidance molecule... | NM_001166287.1 | 99.8% | 99.7% | 1269C>G;1301G>C |
| 3 | human | 56963 | RGMA | repulsive guidance molecule... | NM_001166288.2 | 99.8% | 99.7% | 1269C>G;1301G>C |
| 4 | human | 56963 | RGMA | repulsive guidance molecule... | NM_001166289.1 | 99.8% | 99.7% | 1269C>G;1301G>C |
| 5 | human | 56963 | RGMA | repulsive guidance molecule... | NM_020211.3 | 96.2% | 96.2% | 1_48del;1317C>G;1349G>C |
| 6 | human | 56963 | RGMA | repulsive guidance molecule... | NM_001166283.1 | 94.6% | 94.5% | 1_72del;1341C>G;1373G>C |
| 7 | mouse | 244058 | Rgma | repulsive guidance molecule... | XM_006540902.1 | 86.5% | 90.4% | (many diffs) |
| 8 | mouse | 244058 | Rgma | repulsive guidance molecule... | NM_177740.5 | 83.5% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tatggggaga ggggcaggac gttcagccct gggattctgg ccgaccctcg 121 ccttccttct ctgcagcttc cccgcagcca cctccccgtg caagatcctc aagtgcaact 181 ctgagttctg gagcgccacg tcgggcagcc acgccccagc ctcagacgac acccccgagt 241 tctgtgcagc cttgcgcagc tacgccctgt gcacgcggcg gacggcccgc acctgccggg 301 gtgacctggc ctaccactcg gccgtccatg gcatagagga cctcatgagc cagcacaact 361 gctccaagga tggccccacc tcgcagccac gcctgcgcac gctcccaccg gccggagaca 421 gccaggagcg ctcggacagc cccgagatct gccattacga gaagagcttt cacaagcact 481 cggccacccc caactacacg cactgtggcc tcttcgggga cccacacctc aggactttca 541 ccgaccgctt ccagacctgc aaggtgcagg gcgcctggcc gctcatcgac aataattacc 601 tgaacgtgca ggtcaccaac acgcctgtgc tgcccggctc agcggccact gccaccagca 661 agctcaccat catcttcaag aacttccagg agtgtgtgga ccagaaggtg taccaggctg 721 agatggacga gctcccggcc gccttcgtgg atggctctaa gaacggtggg gacaagcacg 781 gggccaacag cctgaagatc actgagaagg tgtcaggcca gcacgtggag atccaggcca 841 agtacatcgg caccaccatc gtggtgcgcc aggtgggccg ctacctgacc tttgccgtcc 901 gcatgccaga ggaagtgGTC AATGCTGTGG AGGACTGGGA CAGCCAGGGT CTCTACCTCT 961 GCCTGCGGGG CTGCCCCCTC AACCAGCAGA TCGACTTCCA GGCCTTCCAC ACCAATGCTG 1021 AGGGCACCGG TGCCCGCAGG CTGGCAGCCG CCAGCCCTGC ACCCACAGCC CCCGAGACCT 1081 TCCCATACGA GACAGCCGTG GCCAAGTGCA AGGAGAAGCT GCCGGTGGAG GACCTGTACT 1141 ACCAGGCCTG CGTCTTCGAC CTCCTCACCA CGGGCGACGT GAACTTCACA CTGGCCGCCT 1201 ACTACGCGTT GGAGGATGTC AAGATGCTCC ACTCCAACAA AGACAAACTG CACCTGTATG 1261 AGAGGACTCG GGACCTGCCA GGCAGGGCGG CTGCGGGGCT GCCCCTGGCC CCCCGGCCCC 1321 TCCTGGGCGC CCTGGTCCCG CTCCTGGCCC TGCTCCCTGT GTTCTCCTAC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAAGCCGAT CACTGAGGCT ACCCGAGACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt