Construct: ORF TRCN0000466679

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008220.1_s317c1
Derived from:
ccsbBroadEn_04524
DNA Barcode:
CAACCTTGGTTTGCCCGTACCTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
N4BP2L1 (90634)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466679
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001079691.2 100% 100%
2 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001286460.2 95.3% 95.5% 519_543delinsA
3 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001286461.2 87.5% 66.8% (many diffs)
4 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353634.2 86.7% 86.7% 393_394ins69
5 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353630.2 80.9% 76.9% 464_569del;612_613insTGAAAGAACGGTA
6 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353632.2 80.4% 64.4% (many diffs)
7 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353635.2 80.3% 61.3% 306_307ins89;417_418insTGAAAGAACGGTA
8 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 XM_017020841.2 72.3% 66.8% (many diffs)
9 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353636.2 67% 56.9% 306_307ins158;348_349insTGAAAGAACGGTA
10 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001286459.2 65.8% 53.4% (many diffs)
11 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_052818.3 65.8% 53.4% (many diffs)
12 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353628.2 65.2% 65.2% 178_453del
13 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353637.2 51.1% 34% (many diffs)
14 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 XM_011535303.2 48.4% 33.4% (many diffs)
15 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353627.2 48% 39.4% (many diffs)
16 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NM_001353633.2 42.5% 30.7% (many diffs)
17 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 XM_017020840.2 42.5% 30.7% (many diffs)
18 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NR_148478.2 16.9% 1_48del;226_296del;639_3062del
19 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NR_148476.2 14.8% (many diffs)
20 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NR_148480.2 14.7% (many diffs)
21 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NR_148475.2 13.9% (many diffs)
22 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NR_148477.2 13.9% (many diffs)
23 human 90634 N4BP2L1 NEDD4 binding protein 2 like 1 NR_148479.2 13.8% (many diffs)
24 mouse 100637 N4bp2l1 NEDD4 binding protein 2-like 1 NM_133898.4 59.2% 50.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
585
ORF length:
519
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggacagtttc cttcaatctt ttgggaggct gagcctccag ccccagcagc
121agcagcagcg gcagcggccg ccccggccgc ccccgcgggg gacacctcct cgccgccaca
181gctttaggaa acacctctac ctccTGCGAG GCCTCCCGGG CTCCGGGAAA ACTACACTGG
241CCAGACAATT GCAGCATGAC TTTCCCAGGG CCCTGATTTT CAGCACGGAT GATTTTTTCT
301TCAGGGAAGA TGGTGCCTAT GAGTTCAATC CTGACTTCCT GGAGGAAGCT CATGAATGGA
361ACCAAAAAAG AGCAAGAAAA GCAATGAGGA ATGGCATATC CCCCATTATT ATTGATAATA
421CCAACCTCCA CGCCTGGGAA ATGAAGCCCT ATGCAGTCAT GGTATTTCAG ACCGAACAAA
481AGAATCTTTT CAGGCTGGAA ATGGACATGG TAGTTTTCAG GCCAGAAATG AAGAAACATT
541CATGGTGTCT CAAGAGAAAA AATCCACCGA ATGAAAGAAC GGTATGCCCA ACTTTCTTGT
601ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
661AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
721GAAAGGACGA CAACCTTGGT TTGCCCGTAC CTGCACGCGT TAAGTCgaca atcaacctct
781ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)