Construct: ORF TRCN0000466692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012529.1_s317c1
- Derived from:
- ccsbBroadEn_11682
- DNA Barcode:
- TATTCTTCGGCCATCACAATACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAD54L2 (23132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23132 | RAD54L2 | RAD54 like 2 | NM_001322253.2 | 92.6% | 92.5% | 1_324del;745C>T |
| 2 | human | 23132 | RAD54L2 | RAD54 like 2 | NM_001322256.2 | 92.6% | 92.5% | 1_324del;745C>T |
| 3 | human | 23132 | RAD54L2 | RAD54 like 2 | NM_015106.4 | 92.6% | 92.5% | 1_324del;745C>T |
| 4 | human | 23132 | RAD54L2 | RAD54 like 2 | NM_001322257.1 | 74.3% | 75.2% | 0_1ins982;32_110del;1123_1124insGGCTAG |
| 5 | mouse | 81000 | Rad54l2 | RAD54 like 2 (S. cerevisiae) | NM_030730.2 | 84.4% | 88.9% | (many diffs) |
| 6 | mouse | 81000 | Rad54l2 | RAD54 like 2 (S. cerevisiae) | XM_017313702.1 | 53.7% | 55.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4143
- ORF length:
- 4077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aagaaacata cgaaagctac tccgggagga tcaattggag cctgttacca 121 aagcagcaca gcaagaagag ttggaaagaa ggaagcgcct ggagcagcag aggaaagatt 181 atgcagcccc tattcctact gttccgctgg agttcctccc tgaggaaatt gctttaaggg 241 caagtgacgg tccccaactg cctcctcggg tcttggccca ggaagtcatt tgtttggaca 301 gtagcagtgg cagtgaggat gaaaaaagca gtcgagatga ggtgattgaa ctgagctctg 361 gagaggagga cactctgcac attgtggaca gcagtgaatc tgtcagtgaa gatgatgagg 421 aagaagagaa gggtggcacc catgtcaatg atgtcttaaa ccagcgtgac gcccttgggc 481 gggtctttgt caacctaaac caccctccag aggaggaaaa tgtcttcctt gccccacagt 541 tggcacgggc tgtgaaacct catcagattg gcgggatccg gttcctttac gataacctag 601 tggagtctct ggagaggttt aagaccagca gtggctttgg ctgtattctg gcccacagca 661 tgggtctggg gaaaactttg caagtgatct ctttcatcga cgtcctcttc cgccacacgc 721 cagccaaaac agtccttgcc attgtgccgg ttaatactct tcagaattgg ctggcagagt 781 tcaacatgtg gcttccacct cctgaagccc tcccggctga caacaagcct gaagaagtcc 841 agcctcggtt ctttaaagtt cacatcttga atgatgagca caagacgatg gcatctcgtg 901 ctaaagtgat ggctgattgg gtgtcagagg gtggcgtgct gctgatgggg tacgagatgt 961 acagactcct cactctgaag aaatcatttg ccacaggtag accgaagaaa accaagaagc 1021 gttctcaccc agtcatcatt gatctagatg aggaagatcg gcagcaggag tttcggagag 1081 agtttgagaa ggctttatgc cgccctggcc ctgatgtagt aatctgtgat gagggacacc 1141 gcatcaaaaa ctgccaggcc agcacctcac aggctctgaa gaatatccgc tctcgccgcc 1201 gggtggtgct gactgggtac cctctgcaaa acaacctcat tgagtactgg tgcatggtgg 1261 actttgtgcg cccagacttc cttggcaccc ggcaggagtt cagcaacatg tttgaacgcc 1321 ctatcctgaa tgggcaatgt attgacagca cacctcagga cgtccgcctc atgcggtacc 1381 ggagccatgt cctgcacagt cttctggagg gctttgtgca gaggagaggc cacactgtgc 1441 tgaagattca tctccctgcc aaggaagaaa atgtgatcct tgtgcggctc tccaagatcc 1501 agcgagattt gtatacacag ttcatggatc gcttccggga ctgtggtagc agcggttggc 1561 tggggctgaa cccccttaag gcattctgtg tgtgttgcaa gatctggaat caccctgatg 1621 tgctgtatga agcccttcag aaggagagct tggccaatga gcaggaccta gacgtggaag 1681 aacttggctc tgcagggacc agtgcccgct gtccaccaca gggaacaaaa ggcaagggag 1741 aggatagcac cttggcttcc tcgatgggag aggcaaccaa tagcaagttc ctacagggcg 1801 ttggcttcaa ccctttccag gagcgaggca acaacattgt cacatatgaa tgggccaagg 1861 accttctgac taattaccag actggagtcc tagaaaactc tcccaagatg gtactgcttt 1921 tccacctgat tgaggaaagt gtgaagcttg gggacaagat ccttgtgttt agccagagtc 1981 tttccacctt ggctctcatc gaggaattcc ttggaaaacg agaagtaccc tgtccacctg 2041 gtaccgaggg gcaaggagca cagaagtggg ttcgaaacat cagctacttc cggctagatg 2101 gtagcacccc tgcctttgag agggagcggc ttattaatca gttcaatgat cccagcaacc 2161 tcaccacctg gctgttcctt ctctctacaa gggccggatg cttgggtgtg aatctgattg 2221 gtgccaaccg agtggtggtg tttgatgctt cctggaaccc ttgccatgat gcccaggcag 2281 tatgtcgggt ataccgttat ggccagaaaa agccctgtta catctatcgc cttgtggctg 2341 attacactct agaaaagaag atctatgacc gtcagatttc caagcagggc atgtcagatc 2401 gggtggtgga tgatctaaat ccaatgctga acttcacacg gaaagaggtg gaaaacctac 2461 tgcactttgt tgagaaggag ccagctcccc aagtttcctt gaacgtaaag gggatcaagg 2521 agtcagtcct gcaactggcc tgtctgaagt accctcacct catcaccaag gagcctttcg 2581 agcatgagtc attgctcttg aaccgaaagg atcacaagct aaccaaggct gagaaaaaag 2641 cagcaaagaa aagctatgag gaagacaaac gcacatcagt cccctatacc cgcccatcgt 2701 atgcgcagta ttaccctgcc agcgatcaga gcctgaccag catccccgcc ttcagccaga 2761 gaaactggca gccaactttg aagggtgatg aaaagcctgt ggccagtgtt cgtcctgtgc 2821 agtccacccc catccccatg atgccccggc atgtcccatt gggaggaagt gtaagctctg 2881 cctccagcac aaatccatcc atgaactttc ccatcaacta cttgcagcgt gcaggagtcc 2941 ttgtgcagaa ggtggtcacc acgacagata ttgttattcc tggactcaac agctccacag 3001 atgtacaggc aagaattaat gctggtgaga gcatccacat catccgtggg acaaaaggga 3061 cgtacatccg taccagtgat ggacggatct ttgctgtccg ggcaactggc aaaccaaagg 3121 ttcctgaaga tggtcggatg gctgcctcag gttcccaggg accttcttgc gagtccacaa 3181 gcaacggcag acacagtgcc tcatcaccca aagcccccga ccctgagggg ctggccaggc 3241 ccgtctctcc tgacagccca gagatcatca gtgagcttca gcagtatgca gatgtggctg 3301 ctgcccggga atcccgtcag agctccccaa gcaccaatgc cgccctgcct ggccccccgg 3361 cccaacttat ggacagcagt gctgttcccg ggacagctct cggaactgag cctcgactag 3421 ggggtcattg cctcaatagt tccctcttgg tgactggcca gccctgtggt gacaggcacc 3481 cagtgctgga cttaaggggc cacaagcgaa agttggccac accacctgct gcccaggagt 3541 catcccgccg gcggtccagg aagggtcatc tgccagcccc cgtgcagccg tatgaacacg 3601 ggtatccagt ctctggcggg tttgccatgc cacccgtctc cttaaaccat aacctcacca 3661 cccccttcac ctcccaggct ggggagaact ccctgtttat gggcagtacc ccctcctact 3721 accagctgtc caatttgctg gcagatgccc gcctggtgtt tccagtgact actgaccctc 3781 tggtgccagc aggccccgtc agttcctctt ccacggctac ctcagtcact gccagcaacc 3841 ccTCCTTCAT GCTCAACCCT TCTGTGCCAG GGATACTACC CAGCTATTCA CTCCCATTCT 3901 CACAGCCACT CCTGTCCGAG CCGAGGATGT TTGCGCCTTT TCCTTCCCCT GTCTTGCCCA 3961 GCAACCTTTC GCGGGGCATG TCTATCTATC CAGGCTACAT GTCCCCACAT GCAGGCTACC 4021 CAGCTGGTGG CCTCCTACGG TCCCAGGTGC CTCCATTTGA CTCTCATGAG GTTGCCGAGG 4081 TTGGGTTCAG CTCCAATGAT GATGAGGATA AAGACGATGA TGTGATAGAG GTCACTGGGA 4141 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 4201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 4261 GGCTTTATAT ATCTTGTGGA AAGGACGATA TTCTTCGGCC ATCACAATAC ACACGCGTTA 4321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt