Construct: ORF TRCN0000466702
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007413.1_s317c1
- Derived from:
- ccsbBroadEn_05650
- DNA Barcode:
- TCCCTAACAGCGACTTTGAGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM166A (401565)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466702
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401565 | FAM166A | family with sequence simila... | NM_001001710.3 | 100% | 100% | |
| 2 | human | 401565 | FAM166A | family with sequence simila... | XM_024447546.1 | 83.6% | 83.6% | 1_105del;377_457del |
| 3 | human | 401565 | FAM166A | family with sequence simila... | XM_024447547.1 | 83.6% | 83.6% | 1_105del;377_457del |
| 4 | human | 401565 | FAM166A | family with sequence simila... | XM_011518695.2 | 79.2% | 79.2% | 1_105del;376_377ins114 |
| 5 | human | 401565 | FAM166A | family with sequence simila... | XM_024447549.1 | 77.9% | 68.9% | (many diffs) |
| 6 | human | 401565 | FAM166A | family with sequence simila... | XM_006717108.3 | 75.5% | 75.5% | 1_105del;376_377ins153 |
| 7 | human | 401565 | FAM166A | family with sequence simila... | XM_024447551.1 | 72.3% | 71.2% | (many diffs) |
| 8 | human | 401565 | FAM166A | family with sequence simila... | XM_024447548.1 | 65.4% | 58.1% | (many diffs) |
| 9 | human | 401565 | FAM166A | family with sequence simila... | XM_024447550.1 | 57.8% | 57.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1020
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaactact cagaaacacg atctcttcac gccggagcct cactatgtcc 121 ctggctatgc cggcttcttt ccgcagctgc gctaccaggt ggggaacacc tatgggcgta 181 ccacggggca gctgctcaca gaccccagtg tgcagaagag cccctgctct gtgctgtccc 241 ccatgtccaa acccaagttc attgaggact tcagccagtc caagcccccg agggttccct 301 gccaagacct gacggagccc tacatccccc actacaccag tctgaagccc tctaagaact 361 ttgagattct gggccagctc ccacccttgg aggtggacgc ccaggagccg ccaggggtag 421 agaacatacc cagacagatt ctgctgcctg caggcttcac gcccgacacc ccgcaccctc 481 cgtgcccacc aggcaggaag ggagactcca gagacttggg acacccagta tacggggaag 541 aggcctggaa gagcgccact cctgtttgcg aggcccccag gcagcaccag ctgtacCACT 601 GCCAGAGGGA TGAGTACCCA CCCCCCGCCC GCCGCCAGCA GGAGACGCTA GATGTGGGCA 661 GCTTCCAGCG GCTGCCGCAG CTGGACCACC CTAACCTAAT CCAACGCAAG GCCATCTCAG 721 GCTATGCTGG CTTCATTCCC CGTTTCACCT GGGTAATGGG GTTGAATTAC CGAGACGGCG 781 TCATGCAAGC CATGGACGAA TTCGACAAGA GCCAGTTCCT GTTTAGAAAC CCCCACTGTG 841 ACCTGGGCGA GAAGCTGCCT GGAACACACT GGCCCAGCAA CCACATCTAC AGCAGCCAAG 901 GCCTGATCCC CTTCTACATG GGGTTCATCC CCGCCATGCA GGACAACTAC GCCCTGACAT 961 TCGGCAACAG CACCCGGAGG GCCTACTGGA AGGAATGGGC AAAGCGAAAC CACACACTAT 1021 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGATCCCT AACAGCGACT TTGAGTTTGA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt