Construct: ORF TRCN0000466718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018943.3_s317c1
- Derived from:
- ccsbBroadEn_15094
- DNA Barcode:
- TGTCTCCGGACCTAACTAATTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PI4K2B (55300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NM_018323.4 | 98.3% | 48.4% | (many diffs) |
| 2 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248174.2 | 97.5% | 48.9% | (many diffs) |
| 3 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248175.4 | 78.6% | 28.9% | (many diffs) |
| 4 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NR_144633.2 | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 810
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggatccc tccgagcccg accggttggc gtccgcggac ggcgggagcc 121 cggaggagga ggaggatggg gagcgggagc cgctgctacc gcggatcgcc tgggcccacc 181 cgcggagagg cgccccaggc agcgccgtga ggctgctgga cgctgccggg gaggagggcg 241 aggccggcga cgaggagctg cccctcccgc ccggggacgt gggggtctcc cggagttcgc 301 ccgccgagct ggaccggagc cgccccgcgg tttcagtaac tattggtact tcagagatga 361 atgcattctt ggatgaccca gaatttgctg atattatgct gagagcagag caagcaatag 421 aagttggaat ttttccagaa agaatctctc aaggttcaag tggaagttac tttgtgaagg 481 atcctaagag gaaaattatt ggtgtgttta aacccaaatc agaagagcct tatggtcaac 541 tcaatccaaa atggaccaaa tatgtccata aggtctgctg cccttgctgc tttggccgag 601 gctgcctgat tcctaatcag gggtaccttt ccgaagcggg tgcctatctt gtggacaaca 661 agcttcatct gagcattgta cctaaaacaa aggtggtttg gcttgtcagt gagacattta 721 actataatgc gattgaccgt gcaaaatcaa gaggcaaaaa gtatgcttta gaaaaaannn 781 ncnaaagttg ggtagaaagt ttcataggat aggactcctc taagtctaaa atatagatgg 841 tcctttcagt tatttgtgaa ggttacaagg aggctgaata ttggcttagg aaattgaagc 901 tgaccctttg cctgagaata ttagaaaaca atttcagtca caatttgaaa gattagttat 961 tttggattac atcatcagaa atacagacag gggcaatgat aattggttag tcagatacga 1021 aaagcagaaa tgtgaaaagg aaattgacca taaggaatca aaatggattg atgatgaaga 1081 attccttatt aaaatagctg caattgataa tggtctagca tttcctttta aacatcctga 1141 tgaatggaga gcatatccat ttcactgggc ttggcttcct caagcaaaag ttccctttTC 1201 TGAAGAAATA AGAAATTTGA TTCTACCATA TATTTCTGAC ATGAACTTTG TGCAAGATTT 1261 ATGTGAAGAT CTCTATGAAC TTTTTAAGAC TGACAAAGGA TTTGACAAAG CCACTTTTGA 1321 AAGTCAGATG TCTGTGATGA GGGGTCAGAT CTTAAACCTT ACTCAGGCAT TGAGAGACGG 1381 GAAGAGTCCT TTCCAGCTAG TACAGATACC TTGTGTGATT GTGGAACGCA GTCAAGGTGG 1441 AAGTCAGGGT CGGATTGTCC ACCTGAGCAA TTCCTTTACC CAGACTGTCA ATTGCAGGAA 1501 GCCATTTTTT TCCTCCTGGT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1561 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1621 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGTCT CCGGACCTAA 1681 CTAATTTTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt