Construct: ORF TRCN0000466775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013383.1_s317c1
- Derived from:
- ccsbBroadEn_01357
- DNA Barcode:
- CTTCGATGTAGACACAACAGCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB1A (5861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466775
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5861 | RAB1A | RAB1A, member RAS oncogene ... | NM_004161.5 | 100% | 100% | |
| 2 | human | 5861 | RAB1A | RAB1A, member RAS oncogene ... | XM_005264468.2 | 84.3% | 84.3% | 192_193ins96 |
| 3 | human | 81876 | RAB1B | RAB1B, member RAS oncogene ... | NM_030981.3 | 74.9% | 91.7% | (many diffs) |
| 4 | human | 5861 | RAB1A | RAB1A, member RAS oncogene ... | NM_015543.1 | 62.9% | 62.9% | 192_193ins228 |
| 5 | mouse | 19324 | Rab1a | RAB1A, member RAS oncogene ... | NM_008996.3 | 95.6% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagcatgaat cccgaatatg attatttatt caagttactt ctgattggcg 121 actcaggggt tggaaagtct tgccttcttc ttaggtttgc agatgataca tatacagaaa 181 gctacatcag cacaattggt gtggatttca aaataagaac tatagagtta gacgggaaaa 241 caatcaagct tcaaatatgg gacacagcag gccaggaaag atttcgaaca atcaccTCCA 301 GTTATTACAG AGGAGCCCAT GGCATCATAG TTGTGTATGA TGTGACAGAT CAGGAGTCCT 361 TCAATAATGT TAAACAGTGG CTGCAGGAAA TAGATCGTTA TGCCAGTGAA AATGTCAACA 421 AATTGTTGGT AGGGAACAAA TGTGATCTGA CCACAAAGAA AGTAGTAGAC TACACAACAG 481 CGAAGGAATT TGCTGATTCC CTTGGAATTC CGTTTTTGGA AACCAGTGCT AAGAATGCAA 541 CGAATGTAGA ACAGTCTTTC ATGACGATGG CAGCTGAGAT TAAAAAGCGA ATGGGTCCCG 601 GAGCAACAGC TGGTGGTGCT GAGAAGTCCA ATGTTAAAAT TCAGAGCACT CCAGTCAAGC 661 AGTCAGGTGG AGGTTGCTGC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTTC GATGTAGACA 841 CAACAGCAAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt