Construct: ORF TRCN0000466797
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004011.1_s317c1
- Derived from:
- BRDN0000393350
- DNA Barcode:
- GGACAAAGAGTTCTATCACCGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SECISBP2 (79048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466797
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_024077.5 | 99.8% | 99.7% | 20G>C;1482A>G;1657A>G |
| 2 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001282688.2 | 99.7% | 99.6% | (many diffs) |
| 3 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_017015122.2 | 96.3% | 96.2% | (many diffs) |
| 4 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354697.2 | 95.4% | 95.3% | (many diffs) |
| 5 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354696.2 | 95.3% | 95.1% | (many diffs) |
| 6 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001282690.1 | 91.9% | 91.9% | 0_1ins204;1278A>G;1453A>G |
| 7 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_011519000.2 | 91.9% | 91.9% | 0_1ins204;1278A>G;1453A>G |
| 8 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_024447666.1 | 91.9% | 91.8% | (many diffs) |
| 9 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354698.2 | 91.8% | 91.6% | (many diffs) |
| 10 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001282689.2 | 91.3% | 88.2% | (many diffs) |
| 11 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_006717282.2 | 91.3% | 88.2% | (many diffs) |
| 12 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_011519001.1 | 88.4% | 88.4% | (many diffs) |
| 13 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_011519002.1 | 87.5% | 87.4% | (many diffs) |
| 14 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_011519003.1 | 87.3% | 87.3% | (many diffs) |
| 15 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_024447667.1 | 86.8% | 83.6% | (many diffs) |
| 16 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_929843.1 | 67.2% | (many diffs) | |
| 17 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354702.2 | 65.3% | 64.1% | (many diffs) |
| 18 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_024447668.1 | 64.9% | 64.8% | 0_1ins897;585A>G;760A>G |
| 19 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_024447669.1 | 64.9% | 64.8% | 0_1ins897;585A>G;760A>G |
| 20 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_001746377.1 | 64.2% | (many diffs) | |
| 21 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_002956807.1 | 61.2% | (many diffs) | |
| 22 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_929840.1 | 57.3% | (many diffs) | |
| 23 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_001746378.1 | 42.6% | (many diffs) | |
| 24 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_001746376.1 | 41.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2628
- ORF length:
- 2562
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcggagggg ccgccggagc ccgaaagcga gggcatcaag ttatcagcag 121 atgtcaaacc atttgtcccc agatttgccg ggctcaatgt ggcatggtta gagtcctcag 181 aagcatgtgt cttccccagc tctgcagcca catactatcc gtttgttcag gaaccaccag 241 tgacagagca gaaaatatat actgaagaca tggcctttgg agcttcaact tttccacctc 301 agtatttatc ttctgagata actcttcatc catatgccta ttctccttat acccttgact 361 ccacacagaa tgtttactca gtgcctggct cccagtatct ttataaccaa cccagttgtt 421 accgaggttt tcaaacagtg aagcatcgaa atgagaacac atgccctctc ccacaagaaa 481 tgaaagctct gtttaagaag aaaacctatg atgagaaaaa aacgtatgat cagcaaaagt 541 ttgacagtga aagggctgat ggaactatat catctgagat aaaatcagct agaggttcac 601 atcatttgtc catttacgct gagaatagtt tgaaatcaga tggttaccat aagcgaacag 661 acaggaaatc cagaatcatt gcaaaaaatg tatctacctc caaacctgag tttgaattta 721 ccacactgga ctttcctgaa ctgcaaggtg cagagaacaa tatgtcagag atacagaagc 781 aacccaagtg gggacctgtc cactctgtct ctaccgacat ttctcttcta agagaagtag 841 taaaaccagc tgcagtgtta tcaaagggtg aaatagtggt gaaaaataac ccaaatgaat 901 ctgtaactgc taatgccgct accaattctc cttcatgtac aagagagtta tcttggacac 961 caatgggtta tgttgttcga cagacattat ctacagaact gtcagcagcc cctaaaaatg 1021 ttacttctat gataaactta aagaccattg cttcatcagc agatcctaaa aatgttagta 1081 taccatcttc tgaagcttta tcttcggatc cttcctacaa caaagaaaaa cacattattc 1141 atcctaccca aaagtctaaa gcatcacaag gtagtgacct tgaacaaaat gaagcctcaa 1201 gaaagaataa gaaaaagaaa gaaaaatcta catcaaaata tgaagtcctg acagttcaag 1261 agcctccaag gattgaagat gccgaggaat ttcccaacct ggcagttgca tctgaaagaa 1321 gagacagaat agagacaccg aaatttcaat ctaagcagca gccacaggat aattttaaaa 1381 ataatgtaaa gaagagccag cttccagtgc agttggactt ggggggcatg ctgacagccc 1441 tggagaagaa gcagcactct cagcatgcaa agcagtcctc caaaccagtg gtagtctcag 1501 ttggagcagt gccagtcctt tccaaagaat gtgcatcagg ggagaggggc cgccgcatga 1561 gtcaaatgaa gaccccgcac aatcccttgg actccagcgc cccactgatg aagaaaggga 1621 agcagaggga gatccccaag gccaagaagc caacctcact gaagaagatt attttgaaag 1681 aacggcaaga gagaaagcag cgtctccaag aaaatgctgt gggtccagct tttaccagtg 1741 atgacacaca agatggagag agtggtggtg atgaccagtt tcccgagcag gcagagctgt 1801 cagggccaga ggggatggac gaactgatct ccactccttc ggttgaggac aagtctgaag 1861 agccaccagg cacagagctc cagagggaca cagaggcctc ccaccttgct cccaatcaca 1921 ccaccttccc taagatccac agccgcagat tcagggatta ctgcagccag atgcttagta 1981 aagaagtgga tgcttgtgtt accgacctac tcaaagaact ggtccgtttc caagaccgta 2041 tgtaccagaa agatccagtc aaggccaaga ctaaacgtcg acttgtgttg gggttgaggg 2101 aggttctcaa acacctgaag ctcaaaaaac tgaaatgtgt cattatttct cccaactgtg 2161 agaagataca gtcaaaaggt gggctggatg acactttgca cacaattatt gattatgcct 2221 gtgagcagaa cattcccttt gtgttTGCTC TCAACCGCAA AGCTCTGGGG CGCAGTTTGA 2281 ATAAGGCAGT TCCTGTCAGT GTGGTGGGGA TCTTCAGCTA TGATGGGGCC CAGGATCAGT 2341 TCCACAAGAT GGTTGAGCTG ACAGTGGCGG CCCGACAGGC GTACAAGACC ATGCTGGAGA 2401 ATGTGCAGCA GGAGCTGGTG GGAGAGCCCA GGCCTCAGGC ACCTCCCAGC CTACCCACAC 2461 AGGGCCCCAG CTGCCCTGCA GAAGATGGCC CCCCAGCCCT GAAAGAAAAA GAAGAGCCAC 2521 ACTACATTGA AATCTGGAAA AAACATCTGG AAGCATACAG TGGATGTACC CTGGAGCTAG 2581 AAGAATCCTT GGAGGCTTCA ACCTCTCAAA TGATGAATTT GAATTTATGC CCAACTTTCT 2641 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2701 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2761 GTGGAAAGGA CGAGGACAAA GAGTTCTATC ACCGTCAACG CGTTAAGTCg acaatcaacc 2821 tctggattac aaaatttgtg aaagatt