Construct: ORF TRCN0000466800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009562.1_s317c1
- Derived from:
- ccsbBroadEn_04223
- DNA Barcode:
- CTACGCCTAACAATAGCTAGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMD2 (80774)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80774 | LIMD2 | LIM domain containing 2 | NM_030576.4 | 100% | 100% | |
| 2 | human | 80774 | LIMD2 | LIM domain containing 2 | XM_005257703.1 | 100% | 100% | |
| 3 | human | 80774 | LIMD2 | LIM domain containing 2 | XM_005257705.4 | 100% | 100% | |
| 4 | human | 80774 | LIMD2 | LIM domain containing 2 | XM_006722124.2 | 100% | 100% | |
| 5 | mouse | 67803 | Limd2 | LIM domain containing 2 | NM_172397.3 | 89% | 95.3% | (many diffs) |
| 6 | mouse | 67803 | Limd2 | LIM domain containing 2 | XM_006534019.3 | 89% | 95.3% | (many diffs) |
| 7 | mouse | 67803 | Limd2 | LIM domain containing 2 | XM_006534020.3 | 89% | 95.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccaggctgca ggagccgccc aggccacccc ctctcatgac gccaaaggcg 121 gcggcagcag cacggtgcag cgctccaagt ccttcagcct gcgggcccag gtgaaggaga 181 ccTGCGCCGC CTGCCAGAAG ACCGTGTACC CCATGGAGCG GCTGGTGGCC GACAAGCTCA 241 TTTTCCACAA CTCTTGCTTC TGCTGCAAGC ACTGTCACAC CAAGCTCAGC CTGGGCAGCT 301 ACGCCGCGCT GCACGGGGAG TTCTACTGCA AACCCCACTT CCAGCAGCTG TTTAAGAGCA 361 AAGGCAACTA CGACGAGGGG TTTGGCCGCA AGCAGCACAA GGAGCTCTGG GCCCACAAGG 421 AGGTGGACCC CGGCACCAAG ACGGCCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT AATATCTTGT GGAAAGGACG ACTACGCCTA 601 ACAATAGCTA GTCAGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 661 agatt