Construct: ORF TRCN0000466811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004761.1_s317c1
- Derived from:
- ccsbBroadEn_03865
- DNA Barcode:
- CACCATCTTCCTCTACTAGCAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSTC (58505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58505 | OSTC | oligosaccharyltransferase c... | NM_021227.4 | 100% | 100% | |
| 2 | human | 58505 | OSTC | oligosaccharyltransferase c... | NM_001267818.2 | 86% | 84.7% | (many diffs) |
| 3 | human | 58505 | OSTC | oligosaccharyltransferase c... | NM_001267817.1 | 54.5% | 53.6% | (many diffs) |
| 4 | human | 202459 | OSTCP1 | oligosaccharyltransferase c... | NR_028496.1 | 34.4% | (many diffs) | |
| 5 | mouse | 66357 | Ostc | oligosaccharyltransferase c... | NM_025509.3 | 89.7% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGA GACTTTGTAC CGTGTCCCGT TCTTAGTGCT CGAATGTCCC AACCTGAAGC 121 TGAAGAAGCC GCCCTGGTTG CACATGCCGT CGGCCATGAC TGTGTATGCT CTGGTGGTGG 181 TGTCTTACTT CCTCATCACC GGAGGAATAA TTTATGATGT TATTGTTGAA CCTCCAAGTG 241 TCGGTTCTAT GACTGATGAA CATGGGCATC AGAGGCCAGT AGCTTTCTTG GCCTACAGAG 301 TAAATGGACA ATATATTATG GAAGGACTTG CATCCAGCTT CCTATTTACA ATGGGAGGTT 361 TAGGTTTCAT AATCCTGGAC CGATCGAATG CACCAAATAT CCCAAAACTC AATAGATTCC 421 TTCTTCTGTT CATTGGATTC GTCTGTGTCC TATTGAGTTT TTTCATGGCT AGAGTATTCA 481 TGAGAATGAA ACTGCCGGGC TATCTGATGG GTTACCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 661 CCATCTTCCT CTACTAGCAA CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt