Construct: ORF TRCN0000466858
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012920.1_s317c1
- Derived from:
- ccsbBroadEn_10748
- DNA Barcode:
- TACTGGATCAGTCGGGGGAAGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRAT (1384)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466858
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_000755.5 | 86.8% | 86.7% | 840_1085del;1114C>A |
| 2 | human | 1384 | CRAT | carnitine O-acetyltransferase | XM_024447414.1 | 85.7% | 85.4% | (many diffs) |
| 3 | human | 1384 | CRAT | carnitine O-acetyltransferase | XM_017014275.1 | 85.3% | 85.3% | (many diffs) |
| 4 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_001346546.2 | 85.3% | 85.6% | (many diffs) |
| 5 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_001257363.3 | 83.4% | 83.3% | 0_1ins63;777_1022del;1051C>A |
| 6 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_004003.4 | 83.4% | 83.3% | 0_1ins63;777_1022del;1051C>A |
| 7 | human | 1384 | CRAT | carnitine O-acetyltransferase | XM_005251708.4 | 83.4% | 83.3% | 0_1ins63;777_1022del;1051C>A |
| 8 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_001346547.2 | 83% | 82.9% | 840_1085del;1114C>A;1592_1593ins72 |
| 9 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_001346549.2 | 80.4% | 80.3% | 171_172ins120;720_965del;994C>A |
| 10 | human | 1384 | CRAT | carnitine O-acetyltransferase | NM_001346548.2 | 79.6% | 79.5% | (many diffs) |
| 11 | mouse | 12908 | Crat | carnitine acetyltransferase | NM_007760.3 | 75.7% | 79.8% | (many diffs) |
| 12 | mouse | 12908 | Crat | carnitine acetyltransferase | XM_006497645.2 | 75.1% | 79.2% | (many diffs) |
| 13 | mouse | 12908 | Crat | carnitine acetyltransferase | XM_006497646.3 | 73.7% | 78.1% | (many diffs) |
| 14 | mouse | 12908 | Crat | carnitine acetyltransferase | XM_006497647.1 | 72.2% | 76.2% | (many diffs) |
| 15 | mouse | 12908 | Crat | carnitine acetyltransferase | XM_006497648.2 | 72.2% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1698
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt agccttcgct gccaggaccg tggtgaagcc tctgggcttc ctgaagccct 121 tctccttgat gaaggcttcc agccgcttca aggcacacca ggatgcactg ccacggctgc 181 ccgtgccccc tctccagcag tccctggacc actacctgaa ggcgctgcag cccatcgtga 241 gtgaggagga gtgggcccac accaagcagc tggtggatga gtttcaggcc tcaggaggtg 301 taggggagcg cctgcagaag gggctggagc gtcgggccag gaagacggag aactggctgt 361 ctgagtggtg gctcaagacc gcctacctcc agtaccgcca gcctgtggtc atctactcga 421 gcccaggcgt gatgctaccc aagcaggact tcgtggacct gcagggtcag ctccgatttg 481 ctgccaaact cattgagggt gtgttggatt tcaaggtcat gattgacaac gagaccctgc 541 ccgtggagta cctggggggg aagccactgt gcatgaacca gtactatcag atcttgtcct 601 cctgccgagt gccgggcccc aagcaggaca cagtcagcaa cttcagcaag accaagaagc 661 ctcccacgca catcaccgtg gtacacaact accagttttt tgagctggat gtgtaccaca 721 gtgacgggac acccctcact gcggatcaga tctttgtgca gctggagaag atctggaact 781 catccctaca gaccaacaag gagcctgtgg gcatcctcac ctccaaccac cgcaactcct 841 gggccaaggc atacaacacc ctcatcaaag acaaggtgaa ccgggattcc gtgcgctcca 901 tccagaagaa acccgagctt gtgcggtctc ccatggtgcc cctgcccatg cccaagaagc 961 tgcggttcaa catcaccccc gagatcaaga gcgacatcga gaaggccaag cagaacctca 1021 gcatcatgat ccaggacctg gatatcaccg tgatggtgtt ccaccatttt ggaaaagact 1081 tccccaagtc ggagaagcta agcccagatg ccttcatcca gatggctttg cagctggcct 1141 actacaggat ctacggacag gcatgtgcca cctatgaaag tgcctccctg cgcatgtttc 1201 acctgggccg caccgacacc atccgctcgg cttccatgga ctcactcacc tttgtcaagg 1261 ccatggatga ctccagcgtc acggagcacc agaaggtgga gctgctgcgg aaggccgtgc 1321 aggcccaccg aggctacacc gaccgggcca tccgcgggga ggcctttgat cgacacctgc 1381 tgggcctgaa gctgcaggcc atcgaggacc tggtgagcat gcccgacatc ttcatggaca 1441 ccTCCTACGC CATCGCCATG CACTTCCACC TCTCCACCAG CCAGGTCCCT GCCAAGACAG 1501 ACTGTGTCAT GTTCTTCGGG CCCGTGGTCC CCGACGGCTA CGGTGTCTGC TATAACCCCA 1561 TGGAGGCCCA CATCAACTTC TCCCTGTCGG CCTACAACAG CTGCGCGGAG ACCAACGCCG 1621 CCCGCCTGGC GCATTACCTG GAGAAGGCGC TCCTGGACAT GCGTGCCCTG CTGCAGAGCC 1681 ACCCCCGGGC CAAGCTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1741 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1801 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACTGGA TCAGTCGGGG 1861 GAAGCAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt