Construct: ORF TRCN0000466930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003258.1_s317c1
- Derived from:
- ccsbBroadEn_02224
- DNA Barcode:
- TCTTGAACCTCGAGATTATGACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A44 (9673)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9673 | SLC25A44 | solute carrier family 25 me... | NM_014655.4 | 100% | 100% | |
| 2 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_017002904.2 | 100% | 100% | |
| 3 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_017002905.2 | 100% | 100% | |
| 4 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_017002906.2 | 100% | 100% | |
| 5 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_024451071.1 | 100% | 100% | |
| 6 | human | 9673 | SLC25A44 | solute carrier family 25 me... | NM_001286184.1 | 97.5% | 97.5% | 623_646del |
| 7 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_006711657.4 | 97.5% | 97.5% | 623_646del |
| 8 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_011510180.1 | 97.5% | 97.5% | 623_646del |
| 9 | human | 9673 | SLC25A44 | solute carrier family 25 me... | XM_011510181.2 | 97.5% | 97.5% | 623_646del |
| 10 | human | 9673 | SLC25A44 | solute carrier family 25 me... | NR_104411.2 | 12.4% | (many diffs) | |
| 11 | mouse | 229517 | Slc25a44 | solute carrier family 25, m... | NM_001145876.2 | 90% | 96.1% | (many diffs) |
| 12 | mouse | 229517 | Slc25a44 | solute carrier family 25, m... | NM_001281795.1 | 90% | 96.1% | (many diffs) |
| 13 | mouse | 229517 | Slc25a44 | solute carrier family 25, m... | NM_178696.5 | 84.8% | 90.6% | (many diffs) |
| 14 | mouse | 229517 | Slc25a44 | solute carrier family 25, m... | XM_006501347.2 | 67.1% | 59.4% | (many diffs) |
| 15 | mouse | 229517 | Slc25a44 | solute carrier family 25, m... | NM_001145877.2 | 55.9% | 60.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1008
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggacaaacgc aacatccaga tcatcgagtg ggaacacctg gacaagaaga 121 agttctacgt gtttggtgtg gcaatgacaa tgatgatccg tgtcagtgtc tacccattca 181 ccctcatccg cacccggttg caagttcaga aggggaagag cctctaccat gggaccttcg 241 atgccttcat caagatcctg cgagcagatg gtatcactgg cctctaccga gggttcctgg 301 tcaatacctt caccctcatc tctggccagt gttatgtcac cacttatgag ctcacccgga 361 agtttgtagc tgactacagc cagagtaaca cagtcaaatc actggtggct ggtggctcag 421 cctcccttgt ggcccagagc atcacagtgc ccattgatgt agtctcccag cacctgatga 481 tgcaacgcaa gggtgagaaa atgggccgct ttcaggtgcg ggggaaccca gagggacaag 541 gggtagttgc ctttggccaa accaaggaca tcatcaggca gatccTGCAG GCTGATGGAC 601 TTCGCGGCTT CTATCGAGGC TATGTGGCTT CACTGCTTAC CTATATCCCA AACAGTGCTG 661 TCTGGTGGCC CTTCTATCAC TTCTATGCAG AGCAGCTCTC CTACCTGTGT CCTAAGGAGT 721 GCCCTCACAT TGTCTTTCAA GCTGTCTCGG GGCCCCTGGC TGCAGCCACT GCCTCCATCC 781 TCACCAATCC CATGGATGTC ATACGAACCC GTGTGCAGGT TGAGGGCAAG AACTCCATCA 841 TCCTGACCTT CAGACAGCTG ATGGCAGAAG AAGGGCCTTG GGGCCTCATG AAGGGCCTCT 901 CGGCCAGAAT CATCTCAGCC ACACCTTCCA CCATTGTCAT TGTGGTGGGC TATGAGAGCC 961 TCAAGAAACT CAGCCTCCGA CCTGAGCTGG TGGACTCGAG ACACTGGTAC CCAACTTTCT 1021 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1081 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1141 GTGGAAAGGA CGATCTTGAA CCTCGAGATT ATGACTCACG CGTTAAGTCg acaatcaacc 1201 tctggattac aaaatttgtg aaagatt