Construct: ORF TRCN0000466931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016236.1_s317c1
- Derived from:
- ccsbBroadEn_12351
- DNA Barcode:
- TAGCCCGGTCTCGACCTCAATAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ODF2L (57489)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466931
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001366779.1 | 14.7% | 14.4% | 111_227del;332_333insA;345_1539del |
| 2 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001366782.1 | 14.7% | 14.4% | 111_227del;332_333insA;345_1539del |
| 3 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001184766.1 | 12.7% | 12.5% | (many diffs) |
| 4 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001366783.1 | 12.4% | 12.1% | (many diffs) |
| 5 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001184765.1 | 12.1% | 11.9% | (many diffs) |
| 6 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_020729.3 | 12.1% | 11.9% | (many diffs) |
| 7 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001007022.3 | 11.8% | 11.6% | (many diffs) |
| 8 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001366781.1 | 11.7% | 11.4% | (many diffs) |
| 9 | human | 57489 | ODF2L | outer dense fiber of sperm ... | NM_001366784.1 | 11.7% | 11.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 224
- ORF end:
- 452
- ORF length:
- 228
- Sequence:
-
1 GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CACACTGAGT GGGTGGAGAC TGAAGTTAGG 61 CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG 121 TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA 181 GGCTAGCATC GATTGATCAA CAAGTTTGTA CAAAAAAGTT GGCATGTTGC TAGAAAATCT 241 AACTGATAAT GAAAGTGAAA ATACTAATCT TAAGAAGAAG GTATTTGAAA AGGAGGCCCA 301 TATCCAAGAA CTTTCTTGTT TGTTTCAGAG TGAAAAGAGC TTAGAAACCA AGATAGCCAA 361 GTGGAACCTG CAATCAAGAA TGAATAAGAA TGAGGCTATA GTGATGAAAG AAGCAAGTAG 421 GCAAAAAACT GTAGCTTTAA AAAAAGGCAT CTACCCAACT TTCTTGTACA AAGTGGTTGA 481 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTTG 541 GCTTTATATA TCTTGTGGAA AGGACGATAG CCCGGTCTCG ACCTCAATAA CACGCGTTAA 601 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t