Construct: ORF TRCN0000466958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014478.1_s317c1
- Derived from:
- ccsbBroadEn_07414
- DNA Barcode:
- TGCTAACGTTGTCCTTGTTTGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF264 (9422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466958
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9422 | ZNF264 | zinc finger protein 264 | NM_003417.5 | 99.8% | 99.6% | 542G>C;548G>A |
| 2 | human | 9422 | ZNF264 | zinc finger protein 264 | XM_011527522.2 | 94.7% | 94.5% | 159_160ins96;446G>C;452G>A |
| 3 | human | 390980 | ZNF805 | zinc finger protein 805 | NM_001023563.4 | 89.4% | 85.3% | (many diffs) |
| 4 | human | 390980 | ZNF805 | zinc finger protein 805 | NM_001145078.2 | 70.3% | 67.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1950
- ORF length:
- 1881
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcagcg gtgctgacgg accgggccca ggtgtctgtg acctttgatg 121 atgtggctgt gactttcacc aaggaggagt gggggcagct ggacctagct cagcggaccc 181 tgtaccagga ggtgatgctg gaaaactgtg ggctcctggt gtctctgggg tgtcctgttc 241 ccaaagctga gctgatctgc cacctagagc atgggcagga gccatggacc aggaaggaag 301 acctctccca agacacctgt ccaggcgaca aaggaaaacc taagaccaca gaacctacca 361 cttgtgagcc agccttgtca gagggaatct cacttcaggg acaagtgaca caaggaaact 421 cagtggactc acagttgggg caagccgagg atcaggatgg gctatcagaa atgcaggaag 481 gacacttcag accaggaata gatccccagg agaagtctcc tgggaagatg agccctgaat 541 gtgatggttt agggacagct gatggtgtgt gttcaaggat tggacaggag caagtctctc 601 caggagatac agtccatagc cataactcat gtgagtcagg taaagatccc atgattcagg 661 aagaggaaaa taactttaaa tgcagtgaat gtggaaaagt atttaacaag aaacacctcc 721 ttgctggaca tgagaaaatt cactctggag ttaagcccta tgaatgcaca gaatgtggga 781 aaacctttat taagagcaca catctcctgc aacatcacat gatccacact ggggagaggc 841 cctatgagtg catggagtgt ggaaaggcct tcaaccgcaa gtcatacctt acccagcacc 901 agcggattca cagtggagag aagccttaca agtgcaatga atgcggaaag gccttcaccc 961 accgctccaa ttttgtcttg cataacagga gacacactgg agaaaaatcc tttgtgtgca 1021 cagaatgtgg ccaagtcttt cgacataggc caggctttct ccggcactat gttgtccaca 1081 gtggtgagaa tccctatgag tgcttggagt gtggcaaggt cttcaaacac aggtcatatc 1141 tcatgtggca ccagcagact cataccgggg agaagcccta tgagtgcagt gaatgtggga 1201 aggtcttctt ggagagtgca gccctgattc accactatgt catccacact ggagagaagc 1261 cctttgagtg cctcgagtgt gggaaggctt tcaaccaccg atcctacctc aagaggcacc 1321 agcggattca cactggggag aagcccttcg tgtgcagtga atgtggaaag gccttcaccc 1381 actgctctac ttttatcttg cataaaaggg cccacactgg agaaaagcct ttcgagtgca 1441 aagagtgtgg gaaagccttt agcaatcgga aggacctcat tcgccacttc agcatccaca 1501 ctggagagaa gccctatgag tgcgtggagt gtggaaaggc cttcacccgc atgtcgggcc 1561 tcacgaggca caagcggatt catagtggag agaagcccta tgaatgtgtt GAGTGTGGGA 1621 AATCGTTTTG CTGGAGCACA AACCTCATTC GACATGCCAT TATCCACACT GGAGAGAAGC 1681 CCTATAAATG TAGTGAATGT GGAAAGGCCT TCAGTCGCAG CTCGTCCCTC ACTCAGCATC 1741 AAAGGATGCA TACTGGGAAA AATCCCATCA GTGTAACAGA TGTGGGAAGA CCTTTTACAA 1801 GTGGACAAAC CTCAGTTACC CTTCGAGAAC TTCTTTTAGG GAAGGACTTT TTGAATGTAA 1861 CCACTGAGGC AAATATTTTG CCAGAGGAAA CATCTTCCTC TGCATCTGAT CAACCATACC 1921 AAAGAGAAAC CCCACAAGTG TCTTCACTGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1981 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2041 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCTA 2101 ACGTTGTCCT TGTTTGCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2161 tgaaagatt