Construct: ORF TRCN0000466971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010589.1_s317c1
- Derived from:
- ccsbBroadEn_05881
- DNA Barcode:
- TTAAGTTAGCCAGTTCCTGTGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCL2L2 (599)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 599 | BCL2L2 | BCL2 like 2 | NM_001199839.1 | 99.8% | 100% | 123G>A |
| 2 | human | 599 | BCL2L2 | BCL2 like 2 | NM_004050.4 | 99.8% | 100% | 123G>A |
| 3 | human | 100529063 | BCL2L2-PABPN1 | BCL2L2-PABPN1 readthrough | NM_001199864.2 | 52.5% | 46.6% | (many diffs) |
| 4 | mouse | 12050 | Bcl2l2 | BCL2-like 2 | NM_007537.1 | 93.6% | 98.9% | (many diffs) |
| 5 | mouse | 12050 | Bcl2l2 | BCL2-like 2 | XM_006518457.3 | 93.6% | 98.9% | (many diffs) |
| 6 | mouse | 12050 | Bcl2l2 | BCL2-like 2 | XM_006518458.2 | 93.6% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 648
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgacccca gcctcggccc cagacacacg ggctctggtg gcagactttg 121 taggttataa gctgaggcag aagggttatg tctgtggagc tggccccggg gagggcccag 181 cagctgaccc actgcaccaa gccatgcggg cagctggaga tgagttcgag acccgcttcc 241 ggcgcacctt ctctgatctg gcggctcagc tgcatgtgac cccaggctca gcccaacaac 301 gcttcaccca ggtctccgat gaactttttc aagggggccc caactggggc cgccttgtag 361 CCTTCTTTGT CTTTGGGGCT GCACTGTGTG CTGAGAGTGT CAACAAGGAG ATGGAACCAC 421 TGGTGGGACA AGTGCAGGAG TGGATGGTGG CCTACCTGGA GACGCGGCTG GCTGACTGGA 481 TCCACAGCAG TGGGGGCTGG GCGGAGTTCA CAGCTCTATA CGGGGACGGG GCCCTGGAGG 541 AGGCGCGGCG TCTGCGGGAG GGGAACTGGG CATCAGTGAG GACAGTGCTG ACGGGGGCCG 601 TGGCACTGGG GGCCCTGGTA ACTGTAGGGG CCTTTTTTGC TAGCAAGTTG CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGATTAAGTT AGCCAGTTCC TGTGTATACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt