Construct: ORF TRCN0000466986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008120.1_s317c1
- Derived from:
- ccsbBroadEn_04839
- DNA Barcode:
- TCACGGGATGAGCTCCAAAAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BU1 (128312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466986
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 100% | 100% | |
| 2 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 91.2% | 95.2% | (many diffs) |
| 3 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 91% | 96% | (many diffs) |
| 4 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 89.6% | 96.8% | (many diffs) |
| 5 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 89.1% | 96% | (many diffs) |
| 6 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 88.3% | 95.2% | (many diffs) |
| 7 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 88.3% | 95.2% | (many diffs) |
| 8 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 87.8% | 94.4% | (many diffs) |
| 9 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 87.8% | 94.4% | (many diffs) |
| 10 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 87.5% | 95.2% | (many diffs) |
| 11 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 87.5% | 95.2% | (many diffs) |
| 12 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 87.5% | 95.2% | (many diffs) |
| 13 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 87.3% | 92.8% | (many diffs) |
| 14 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 87.3% | 92.8% | (many diffs) |
| 15 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 87% | 95.2% | (many diffs) |
| 16 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 86.5% | 96% | (many diffs) |
| 17 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 86.5% | 95.2% | (many diffs) |
| 18 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 85.9% | 95.2% | (many diffs) |
| 19 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 85.8% | 89.5% | (many diffs) |
| 20 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 85.7% | 96% | (many diffs) |
| 21 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 85.4% | 95.2% | (many diffs) |
| 22 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 84.9% | 92.8% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 73.5% | 80% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 68.5% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 58.2% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 21.4% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 18.5% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.4% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 16% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.5% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 14% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.5% | (many diffs) | |
| 33 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 90.2% | 96% | (many diffs) |
| 34 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 90.2% | 96% | (many diffs) |
| 35 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 89.9% | 94.4% | (many diffs) |
| 36 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 89.9% | 96.8% | (many diffs) |
| 37 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 89.9% | 95.2% | (many diffs) |
| 38 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 89.9% | 95.2% | (many diffs) |
| 39 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 89.9% | 95.2% | (many diffs) |
| 40 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 89.6% | 96% | (many diffs) |
| 41 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 89.6% | 96% | (many diffs) |
| 42 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 89.6% | 96% | (many diffs) |
| 43 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 89.6% | 95.2% | (many diffs) |
| 44 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 89.4% | 95.2% | (many diffs) |
| 45 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 89.1% | 95.2% | (many diffs) |
| 46 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 89.1% | 95.2% | (many diffs) |
| 47 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 89.1% | 96% | (many diffs) |
| 48 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 89.1% | 95.2% | (many diffs) |
| 49 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 89.1% | 95.2% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 89.1% | 95.2% | (many diffs) |
| 51 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 88.8% | 95.2% | (many diffs) |
| 52 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 87% | 93.6% | (many diffs) |
| 53 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 85.4% | 95.2% | (many diffs) |
| 54 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 84% | 89.5% | (many diffs) |
| 55 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 84% | 89.5% | (many diffs) |
| 56 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 83.8% | 86.6% | (many diffs) |
| 57 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 81.2% | 86.9% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 74.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 447
- ORF length:
- 378
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GCCAGACCCG TCCAAATCGG CTCCTGCGCC CAAGAAGGGT TCTAAAAAGG 121 CTGTCACCAA GGCACAGAAG AAGGACGGCA AGAAGCGCAA GCGCGGCCGC AAGGAGAGCT 181 ATTCTATCTA CGTGTACAAG GTGCTGAAGC AGGTGCACCC CGACACCGGC ATCTCGTCCA 241 AGGCCATGGG CATCATGAAC TCCTTCGTCA ATGACATCTT CGAGCGCATC GCCAGCGAGG 301 CCTCCCGCCT GGCACACTAC AACAAGCGCT CCACCATCAC GTCCCGCGAA GTGCAGACGG 361 CCGTTCGCCT GCTGCTGCCC GGCGAGCTGG CCAAGCACGC CGTGTCCGAG GGCACCAAGG 421 CTGTCACCAA GTACACCAGC TCCAAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCACGGGA 601 TGAGCTCCAA AAATGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt