Construct: ORF TRCN0000466993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015499.1_s317c1
- Derived from:
- ccsbBroadEn_02747
- DNA Barcode:
- CTGAGGTAACTTTCACCTCCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGRN1 (23295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466993
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23295 | MGRN1 | mahogunin ring finger 1 | NM_015246.4 | 100% | 100% | |
| 2 | human | 23295 | MGRN1 | mahogunin ring finger 1 | NM_001142289.2 | 96.1% | 96.1% | 1065_1066ins66 |
| 3 | human | 23295 | MGRN1 | mahogunin ring finger 1 | NM_001142290.2 | 95.4% | 93.9% | (many diffs) |
| 4 | human | 23295 | MGRN1 | mahogunin ring finger 1 | NM_001142291.2 | 91.6% | 90.1% | (many diffs) |
| 5 | human | 23295 | MGRN1 | mahogunin ring finger 1 | NR_102267.1 | 39.4% | 1_137del;1092_1093ins176;1690_3758del | |
| 6 | mouse | 17237 | Mgrn1 | mahogunin, ring finger 1 | XM_006521832.1 | 85.5% | 88.6% | (many diffs) |
| 7 | mouse | 17237 | Mgrn1 | mahogunin, ring finger 1 | XM_017316887.1 | 82% | 85% | (many diffs) |
| 8 | mouse | 17237 | Mgrn1 | mahogunin, ring finger 1 | XM_006521834.1 | 81.9% | 84.9% | (many diffs) |
| 9 | mouse | 17237 | Mgrn1 | mahogunin, ring finger 1 | NM_029657.4 | 78.4% | 81.3% | (many diffs) |
| 10 | mouse | 17237 | Mgrn1 | mahogunin, ring finger 1 | NM_001252437.1 | 78.3% | 81.2% | (many diffs) |
| 11 | mouse | 17237 | Mgrn1 | mahogunin, ring finger 1 | XM_006521837.1 | 63.6% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1794
- ORF length:
- 1728
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctccattctc agccgccgca tcgcgggggt ggaggacatc gacatccagg 121 cgaactcggc ctatcgctac cctccgaagt ccggaaacta ctttgcttcg cactttttca 181 tgggaggaga gaaattcgac accccccacc ctgaaggtta cctctttgga gagaacatgg 241 atctgaactt cctgggcagc cgcccggtcc agtttcccta cgtcactcct gccccccacg 301 agcccgtgaa gacgctgcgg agcctggtga acatccgcaa agactccctg cggctggtga 361 ggtacaaaga cgatgccgac agccccaccg aggacggcga caagccccgg gtgctctaca 421 gcctggagtt caccttcgac gccgatgccc gcgtggccat caccatctac tgccaggcat 481 cggaggagtt cctgaacggc agggcagtat acagccccaa gagcccctcg ctacagtccg 541 agaccgtcca ctacaagaga ggggtgagcc agcagttctc cctgccctcc ttcaagattg 601 acttctcgga atggaaggat gacgagctga actttgacct ggaccggggc gtgtttccag 661 tagtcatcca ggctgtggtg gacgaaggag atgtggtgga agtgactggc cacgcccacg 721 tgctcttggc tgcctttgaa aagcacatgg acggcagctt ctctgtgaag cctttaaagc 781 agaagcaaat tgtggaccgg gtcagctacc tcctgcagga gatctatggc attgagaaca 841 agaacaacca ggagaccaag ccctcggacg acgagaacag cgacaacagc aacgagtgtg 901 tggtgtgcct gtccgacctg cgggacacgc tgatcctgcc ctgccgccac ctgtgcctct 961 gtacctcctg cgccgacacg ctgcgctacc aggccaacaa ctgccccatc tgccggctgc 1021 ctttccgggc cctcctgcag atccgggcgg tgcggaagaa gccaggagcc ctgtcccccg 1081 tgtccttcag ccccgtcctg gcccagagcc tggagcatga tgagcactct tgtcccttta 1141 aaaaatcaaa gccgcacccc gcctccctgg ccagcaagaa acctaaaagg gaaacaaact 1201 ctgacagcgt cccacctggc tacgagccca tctcgctgct cgaggcgctc aacggcctcc 1261 gggctgtctc cccggccatc ccctcggccc ctctttatga agaaatcacc tattcaggca 1321 tctcggacgg cctgtcccag gccagctgtc ccctcgcggc tatcgaccac atcctggaca 1381 gcagccgcca gaagggcagg ccgcagagca aggcccccga cagcacccta cggtccccgT 1441 CTTCCCCCAT CCACGAAGAG GATGAGGAGA AGCTCTCCGA GGACGTGGAC GCCCCTCCCC 1501 CACTGGGTGG CGCAGAGCTG GCCCTGCGGG AAAGCAGCTC CCCTGAGAGT TTCATAACAG 1561 AAGAGGTTGA TGAGTCGTCG TCACCACAGC AAGGGACCCG AGCAGCTTCC ATTGAGAATG 1621 TCCTGCAGGA CAGCAGCCCC GAGCACTGTG GCCGAGGCCC ACCTGCTGAC ATCTACCTGC 1681 CAGGACGGCC CACCTCCATG GAGACGGCCC ACGGCCTCGC CACCACCAGC CCCACCTGGC 1741 CTCCACTTGG TGGCCCCAGC CCCGATCCCA GCGCCGCCGA GCTGACCCCA CTCTGCCCAA 1801 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1861 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1921 TATCTTGTGG AAAGGACGAC TGAGGTAACT TTCACCTCCA CCTACGCGTT AAGTCgacaa 1981 tcaacctctg gattacaaaa tttgtgaaag att