Construct: ORF TRCN0000467032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001344.1_s317c1
- Derived from:
- ccsbBroadEn_02155
- DNA Barcode:
- AATGGGATCCGGTGGTTCCAGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAP2 (9402)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9402 | GRAP2 | GRB2 related adaptor protein 2 | NM_001291824.2 | 100% | 100% | |
| 2 | human | 9402 | GRAP2 | GRB2 related adaptor protein 2 | NM_001291825.1 | 100% | 100% | |
| 3 | human | 9402 | GRAP2 | GRB2 related adaptor protein 2 | NM_004810.4 | 100% | 100% | |
| 4 | human | 9402 | GRAP2 | GRB2 related adaptor protein 2 | NM_001291826.2 | 78.1% | 78.1% | 0_1ins216 |
| 5 | human | 9402 | GRAP2 | GRB2 related adaptor protein 2 | XM_006724376.3 | 78.1% | 78.1% | 0_1ins216 |
| 6 | human | 9402 | GRAP2 | GRB2 related adaptor protein 2 | NM_001291828.2 | 65.7% | 56.6% | 0_1ins170;117_118ins169 |
| 7 | mouse | 17444 | Grap2 | GRB2-related adaptor protein 2 | NM_001289442.1 | 81.8% | 86% | (many diffs) |
| 8 | mouse | 17444 | Grap2 | GRB2-related adaptor protein 2 | NM_010815.3 | 81.8% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agctgttgcc aagtttgatt tcactgcttc aggtgaggat gaactgagct 121 ttcacactgg agatgttttg aagattttaa gtaaccaaga ggagtggttt aaggcggagc 181 ttgggagcca ggaaggatat gtgcccaaga atttcataga catccagttt cccaaatggt 241 ttcacgaagg cctctctcga caccaggcag agaacttact catgggcaag gaggttggct 301 tcttcatcat ccgggccagc cagagctccc caggggactt ctccatctct gtcaggcatg 361 aggatgacgt tcaacacttc aaggtcatgc gagacaacaa gggtaattac tttctgtgga 421 ctgagaagtt tccatcccta aataagctgg tagactacta caggacaaat tccatctcca 481 gacagaagca gatcttcctt agagacagaa cccgagaaga ccagggtcac cggggcaaca 541 gcctggaccg gaggtcccag ggaggcccac acctcagtgg ggctgtggga gaagaaatcc 601 gaccttcgat gaaccggaag ctgtcggatc accccccgac ccttcccctg cagcagcacc 661 agcaccagcc acagcctccg caatatgccc cagcgcccca gcagctgcag cagcccccac 721 agcagcgata tctgcagcac caCCATTTCC ACCAGGAACG CCGAGGAGGC AGCCTTGACA 781 TAAATGATGG GCATTGTGGC ACCGGCTTGG GCAGTGAAAT GAATGCGGCC CTCATGCATC 841 GGAGACACAC AGACCCAGTG CAGCTCCAGG CGGCAGGGCG AGTGCGGTGG GCCCGGGCGC 901 TGTATGACTT TGAGGCCCTG GAGGATGACG AGCTGGGGTT CCACAGCGGG GAGGTGGTGG 961 AGGTCCTGGA TAGCTCCAAC CCATCCTGGT GGACCGGCCG CCTGCACAAC AAGCTGGGCC 1021 TCTTCCCTGC CAACTACGTG GCACCCATGA CCCGATACCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AAATGGGATC CGGTGGTTCC AGCAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt