Construct: ORF TRCN0000467032

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001344.1_s317c1
Derived from:
ccsbBroadEn_02155
DNA Barcode:
AATGGGATCCGGTGGTTCCAGCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRAP2 (9402)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467032
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9402 GRAP2 GRB2 related adaptor protein 2 NM_001291824.2 100% 100%
2 human 9402 GRAP2 GRB2 related adaptor protein 2 NM_001291825.1 100% 100%
3 human 9402 GRAP2 GRB2 related adaptor protein 2 NM_004810.4 100% 100%
4 human 9402 GRAP2 GRB2 related adaptor protein 2 NM_001291826.2 78.1% 78.1% 0_1ins216
5 human 9402 GRAP2 GRB2 related adaptor protein 2 XM_006724376.3 78.1% 78.1% 0_1ins216
6 human 9402 GRAP2 GRB2 related adaptor protein 2 NM_001291828.2 65.7% 56.6% 0_1ins170;117_118ins169
7 mouse 17444 Grap2 GRB2-related adaptor protein 2 NM_001289442.1 81.8% 86% (many diffs)
8 mouse 17444 Grap2 GRB2-related adaptor protein 2 NM_010815.3 81.8% 86% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1056
ORF length:
990
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agctgttgcc aagtttgatt tcactgcttc aggtgaggat gaactgagct
121ttcacactgg agatgttttg aagattttaa gtaaccaaga ggagtggttt aaggcggagc
181ttgggagcca ggaaggatat gtgcccaaga atttcataga catccagttt cccaaatggt
241ttcacgaagg cctctctcga caccaggcag agaacttact catgggcaag gaggttggct
301tcttcatcat ccgggccagc cagagctccc caggggactt ctccatctct gtcaggcatg
361aggatgacgt tcaacacttc aaggtcatgc gagacaacaa gggtaattac tttctgtgga
421ctgagaagtt tccatcccta aataagctgg tagactacta caggacaaat tccatctcca
481gacagaagca gatcttcctt agagacagaa cccgagaaga ccagggtcac cggggcaaca
541gcctggaccg gaggtcccag ggaggcccac acctcagtgg ggctgtggga gaagaaatcc
601gaccttcgat gaaccggaag ctgtcggatc accccccgac ccttcccctg cagcagcacc
661agcaccagcc acagcctccg caatatgccc cagcgcccca gcagctgcag cagcccccac
721agcagcgata tctgcagcac caCCATTTCC ACCAGGAACG CCGAGGAGGC AGCCTTGACA
781TAAATGATGG GCATTGTGGC ACCGGCTTGG GCAGTGAAAT GAATGCGGCC CTCATGCATC
841GGAGACACAC AGACCCAGTG CAGCTCCAGG CGGCAGGGCG AGTGCGGTGG GCCCGGGCGC
901TGTATGACTT TGAGGCCCTG GAGGATGACG AGCTGGGGTT CCACAGCGGG GAGGTGGTGG
961AGGTCCTGGA TAGCTCCAAC CCATCCTGGT GGACCGGCCG CCTGCACAAC AAGCTGGGCC
1021TCTTCCCTGC CAACTACGTG GCACCCATGA CCCGATACCC AACTTTCTTG TACAAAGTGG
1081TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1141CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1201AAATGGGATC CGGTGGTTCC AGCAGACGCG TTAAGTCgac aatcaacctc tggattacaa
1261aatttgtgaa agatt

Download FASTA (ORF) (Full)