Construct: ORF TRCN0000467054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008669.1_s317c1
- Derived from:
- BRDN0000395088
- DNA Barcode:
- AGTCATTATCCACTGCTTCTACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOCK8 (81704)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | NM_001190458.2 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_011518045.3 | 99.7% | 99.5% | (many diffs) |
| 3 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | NM_001193536.1 | 98.1% | 97.9% | (many diffs) |
| 4 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_011518047.3 | 98.1% | 97.9% | (many diffs) |
| 5 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_011518048.2 | 98.1% | 97.9% | (many diffs) |
| 6 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_017015173.1 | 98.1% | 97.9% | (many diffs) |
| 7 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_011518046.2 | 97.1% | 96.9% | (many diffs) |
| 8 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_017015174.1 | 97.1% | 96.9% | (many diffs) |
| 9 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | NM_203447.3 | 95% | 94.8% | (many diffs) |
| 10 | human | 81704 | DOCK8 | dedicator of cytokinesis 8 | XM_011518049.2 | 72.6% | 72.6% | (many diffs) |
| 11 | mouse | 76088 | Dock8 | dedicator of cytokinesis 8 | XM_006527435.3 | 85.5% | 90.2% | (many diffs) |
| 12 | mouse | 76088 | Dock8 | dedicator of cytokinesis 8 | XM_011247397.2 | 85.5% | 90.2% | (many diffs) |
| 13 | mouse | 76088 | Dock8 | dedicator of cytokinesis 8 | NM_028785.3 | 82.8% | 87.2% | (many diffs) |
| 14 | mouse | 76088 | Dock8 | dedicator of cytokinesis 8 | XM_006527436.3 | 42.1% | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 6066
- ORF length:
- 5997
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacacacctg aacagcctgg aggggcggct tgcccaggag ctcggggact 121 tcactgatga cgacttggac gtggtgttca cgccaaagga atgtaggact ttgcagccct 181 ctttgccgga ggaaggggtt gaactggacc ctcatgtcag ggactgtgtt cagacctaca 241 tccgtgagtg gctaatcgtg aaccggaaaa accaaggaag tccagaaatc tgtggcttta 301 aaaagactgg atctcgaaaa gattttcaca agacgcttcc gaaacagacg tttgagtcgg 361 aaaccttgga gtgcagtgaa cccgctgctc aggcaggccc ccgccactta aacgtgctgt 421 gcgacgtgtc tgggaaaggc cccgtcactg cctgtgactt tgacctccgc agcctgcagc 481 ctgacaagcg gctagaaaac ctcctgcagc aagtgagtgc cgaggacttt gagaagcaga 541 acgaggaggc ccggaggacc aataggcagg ccgagctctt tgccctttac ccatcagtgg 601 acgaggagga tgctgtggaa atacgtccag taccagaatg tcccaaggaa cacctgggca 661 acagaatatt ggtcaagttg ctgaccttga agttcgagat tgaaattgag cccctgtttg 721 ccagcattgc cctctacgat gttaaagaaa ggaaaaagat ctcagaaaat tttcactgtg 781 acctgaactc tgaccagttc aaaggatttc tgcgagctca cacgccttca gtggccgcat 841 caagtcaggc gagatctgca gtcttctcag tcacctaccc gtcctcagac atctacctgg 901 tagtcaagat tgaaaaagtc ctgcagcagg gagagattgg agactgtgca gagccctaca 961 cggttatcaa agaaagtgat ggtggaaaga gtaaagaaaa gattgaaaaa ctaaaactcc 1021 aagctgaatc cttctgccag cgtttgggga aataccggat gccctttgcc tgggcaccca 1081 taagcttatc aagcttcttc agtgtctcca cccttgagag ggaggtaact gatgtggact 1141 ctgtggttgg gagaagctca gtgggtgaac ggaggacatt ggcccaatct agaaggcttt 1201 ctgaaagagc cctctccttg gaggaaaatg gggttggatc caacttcaaa acctccactc 1261 tgagcgttag catctttttc aagcaggaag gagatcgcct tagcgatgaa gacttattca 1321 agtttttagc tgactacaaa agatcatcat ccttacagag acgagtcaag tcaattccag 1381 gcttgctaag actggagatt tctacagctc cagagatcat caattgctgt ctgactcctg 1441 aaatgctgcc cgtgaaaccc tttcctgaaa accggacacg cccgcacaaa gagattttgg 1501 aatttccaac acgagaagta tatgtccctc acactgtgta cagaaacctt ctctatgtct 1561 acccacagag gctgaacttt gtaaacaaac tagcatcagc ccggaacatt acaataaaga 1621 tccagtttat gtgtggagaa gatgctagca atgcgatgcc ggtcatcttt ggaaagtcca 1681 gcgggcctga atttctgcag gaagtgtaca cagctgttac ataccataat aagtctcctg 1741 acttttatga agaagtgaaa attaagctcc ccgctaagct cacagtaaat caccacctcc 1801 tgttcacctt ctaccatatc agctgtcagc agaagcaagg agcctccgtg gaaactctcc 1861 tgggatattc atggctgcca attctcttaa atgaacgtct tcaaactgga tcctactgtc 1921 tcccagttgc cttggaaaaa ttgccaccca actactccat gcattctgct gagaaagtcc 1981 cattacagaa tcctcccatt aagtgggctg aaggacataa gggagtattt aatattgaag 2041 tgcaagctgt ttcttctgta cacacccagg acaaccacct ggagaagttc ttcaccctct 2101 gccactccct ggagagccag gtgaccttcc ccatccgcgt gctggatcag aaaatcagcg 2161 agatggcgct ggagcatgag ctgaagctca gcatcatctg cctcaactcc tcccgcctgg 2221 agccgctcgt gctcttcctg cacctggtgc tggacaagct cttccagctg tccgtgcagc 2281 ccatggtcat cgctggccag acagccaact tctcccagtt tgccttcgag tccgtggtgg 2341 ccatcgccaa cagtctgcac aacagcaagg acctgagcaa ggaccagcat gggaggaact 2401 gcctgctggc ttcctacgtg cactacgtct tccgcctgcc agaggtgcaa agggatgtgc 2461 ccaagtcagg cgctcccact gccctcctag accctcggag ctaccacacg tatggccgca 2521 catcagctgc tgctgtgagt tcaaagctgc tgcaggcccg ggtgatgagc agcagtaacc 2581 cagacctcgc ggggacacac tccgcagcag acgaggaagt gaagaacatc atgtcttcaa 2641 agcatttcca tgaggagctt gcccttcaga tggtggtcag cactggaatg gtgagagaaa 2701 cagtcttcaa gtatgcctgg ttcttctttg agcttctggt gaaaagcatg gcccagcacg 2761 tacataacat ggacaaacgg gacagttttc ggaggactcg tttttctgac cgtttcatgg 2821 atgacataac tactattgtt aatgtggtca cctcggaaat tgcagccctt ttagtaaaac 2881 cacagaagga aaatgaacag gcggaaaaga tgaacatcag cctggctttc ttcttgtatg 2941 accttctctc cctcatggat cggggctttg tgtttaacct catcagacat tattgcagcc 3001 agctgtcagc caagctcagt aaccttccaa cgctcatttc catgaggcta gagttcctga 3061 gaatcctctg tagccatgag cattacctca atctgaacct tttttttatg aatgctgata 3121 ctgctccaac atctccttgt ccttccatat cttcccagaa ctcaagctcc tgctccagct 3181 tccaggacca gaagatcgcc agcatgttcg atctgacttc cgagtaccgc cagcagcact 3241 tcctcaccgg gctcctcttc acagaactgg ctgctgccct ggatgccgaa ggggaaggaa 3301 tcagcaaagt acaaaggaaa gctgtcagtg caattcacag cctgctaagt tctcacgacc 3361 tggacccacg ctgtgtcaaa ccagaggtga aggtcaaaat cgccgccctt tacctacctt 3421 tagttggcat cattttggat gctttgccac agctctgtga ctttacagtt gcagatactc 3481 gcagataccg caccagtggc tcggatgaag aacaagaagg agccggtgcc attaaccaga 3541 atgtggctct ggccatagca gggaataatt tcaatttgaa aacaagtgga atagtgctgt 3601 cttccttgcc ctataagcag tacaacatgc tgaacgcgga cactactcgc aacctcatga 3661 tctgcttcct ctggatcatg aaaaatgctg atcagagcct cattaggaag tggattgctg 3721 acctgccatc aacgcagctc aacaggattt tagatctact tttcatctgt gtgttatgtt 3781 ttgagtataa gggaaaacag agttctgaca aagtcagtac ccaagtcctg cagaagtcaa 3841 gggatgtcaa ggcccggctg gaagaggctt tgctccgtgg ggaaggggcc agaggggaga 3901 tgatgcgccg ccgggctcca gggaacgacc gatttccagg cctaaatgaa aatttgagat 3961 ggaagaaaga gcagacacat tggcggcaag ctaatgagaa gctagataaa acaaaggccg 4021 agttagatca agaagccttg atcagtggca atctggctac agaagcacat ttaatcatcc 4081 tggatatgca ggaaaacatt atccaggcga gctcggctct ggactgtaaa gacagcctgc 4141 tgggaggtgt tctgagggtg ctggtgaatt ctctgaactg tgatcagagt accacctacc 4201 tgactcactg ctttgcaaca ctccgtgctc tcatcgccaa gtttggagac ttactcttcg 4261 aagaggaggt ggaacagtgt ttcgacctat gtcaccaagt cctgcaccac tgcagcagca 4321 gcatggatgt cacccggagc caagcctgtg ccacccttta cctcctcatg aggttcagtt 4381 ttggagccac cagtaatttt gcaagagtaa agatgcaagt aaccatgtcc ctggcatctt 4441 tggtgggaag agcaccagac tttaatgaag agcacctgag aagatccttg aggacaattt 4501 tggcctattc agaagaggac acagccatgc agatgactcc ttttcccacc caggtggagg 4561 aacttctctg taatctgaat agcatcttat atgacacagt gaaaatgagg gaatttcagg 4621 aagatcctga gatgcttatg gatctcatgt acagaattgc caagagttac caggcatctc 4681 ctgatctgcg gctgacctgg ctccagaaca tggcagagaa acacaccaag aagaagtgct 4741 acacggaggc tgccatgtgc ctggtgcacg ccgctgcgtt agtggctgag tatctgagca 4801 tgctggagga ccacagctac ctgcccgtgg gcagtgtcag cttccagaat atttcttcca 4861 atgtgctgga ggagtctgtg gtctctgagg acaccctgtc acctgacgag gatggggtgt 4921 gcgcaggcca gtacttcacc gagagtggcc tggtaggcct cctggagcag gccgcggagc 4981 tcttcagcac gggaggctta tatgagacag ttaatgaggt ctacaagctg gtcatcccca 5041 tcctagaagc gcatcgagaa ttccggaagc tgacactcac tcacagcaag ctgcagagag 5101 ccttcgacag catcgttaac aaggatcata agagaatgtt tggaacctac ttccgagttg 5161 gtttctttgg atccaaattt ggggatttgg atgaacagga atttgtctac aaagagcctg 5221 caattaccaa gcttcctgag atctcacata gactagaggc attttatggt caatgttttg 5281 gtgcagaatt tgtggaagtg attaaagact ccactcctgt ggacaaaacc aagttggatc 5341 ctaacaaggc ctacatacag atcacttttg tggagcccta ctttgatgag tatgagatga 5401 aagacagggt cacatacttt gagaagaatt tcaacctccg gaggttcatg tacaccaccc 5461 tgaacagcct ggaggggcgg cctcggggag agctgcatga gcagtacaga aggaacacag 5521 tcctgaccac tatgcacgcc ttccccttca tcaagaccag gatcagcgtc atccagaagg 5581 aggagtttgt tttgacaccg attgaagttg ccattgaaga catgaagaag aagaccctgc 5641 agttagcagt tgccattaac caggagccgc cTGATGCAAA GATGCTTCAG ATGGTGCTGC 5701 AAGGCTCTGT GGGAGCTACT GTAAATCAGG GACCACTGGA AGTAGCCCAA GTGTTTTTGG 5761 CTGAAATTCC TGCTGATCCA AAACTCTATC GACATCACAA CAAGTTGAGG TTATGCTTTA 5821 AGGAATTCAT CATGAGATGT GGTGAAGCTG TAGAGAAAAA CAAGCGTCTC ATCACGGCAG 5881 ACCAGAGGGA ATATCAGCAG GAACTCAAAA AGAACTATAA CAAGCTAAAA GAGAACCTCA 5941 GGCCAATGAT CGAGCGGAAA ATTCCAGAAC TGTACAAGCC AATATTCAGA GTTGAGAGTC 6001 AAAAGAGGGA CTCCTTCCAC AGATCTAGTT TCAGGAAATG TGAAACCCAG TTGTCACAGG 6061 GCAGCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 6121 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 6181 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTCATTAT CCACTGCTTC TACTGACGCG 6241 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt